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Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy
BACKGROUND: Pregnancy-induced immunological changes contribute to the maternal immune tolerance. Nuclear factor kappa B (NF-κB) pathway participates in regulating both innate and adaptive immunities, and lymph nodes play key roles in adaptive immune reaction. However, it is unclear whether early pre...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275262/ https://www.ncbi.nlm.nih.gov/pubmed/35821130 http://dx.doi.org/10.1186/s12917-022-03373-7 |
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author | Zhang, Leying Zhang, Taipeng Yang, Zhen Cai, Chunjiang Hao, Shaopeng Yang, Ling |
author_facet | Zhang, Leying Zhang, Taipeng Yang, Zhen Cai, Chunjiang Hao, Shaopeng Yang, Ling |
author_sort | Zhang, Leying |
collection | PubMed |
description | BACKGROUND: Pregnancy-induced immunological changes contribute to the maternal immune tolerance. Nuclear factor kappa B (NF-κB) pathway participates in regulating both innate and adaptive immunities, and lymph nodes play key roles in adaptive immune reaction. However, it is unclear whether early pregnancy changes the expression of NF-κB family in maternal lymph node in sheep. METHODS: In this study, the samples of inguinal lymph nodes were collected from ewes on day 16 of the estrous cycle, and on days 13, 16 and 25 of pregnancy, and expression of NF-κB family, including NF-κB p105 (NFKB1), NF-κB p100 (NFKB2), p65 (RELA), RelB (RELB) and c-Rel (REL), were analyzed through real-time quantitative PCR, Western blot and immunohistochemical analysis. RESULTS: The expression levels of NF-κB p105 and c-Rel downregulated, but NF-κB p100 upregulated on day 25 of pregnancy. The expression levels of p65, RelB and c-Rel peaked at day 13 of pregnancy, and expression level of RelB was higher during early pregnancy comparing to day 16 of the estrous cycle. In addition, p65 protein was located in the subcapsular sinus and lymph sinuses. CONCLUSION: This paper reported for the first time that early pregnancy has effects on the expression of NF-κB family, which may contribute to the maternal immunoregulation through blood circulation and lymph circulation during early pregnancy in sheep. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03373-7. |
format | Online Article Text |
id | pubmed-9275262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92752622022-07-13 Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy Zhang, Leying Zhang, Taipeng Yang, Zhen Cai, Chunjiang Hao, Shaopeng Yang, Ling BMC Vet Res Research BACKGROUND: Pregnancy-induced immunological changes contribute to the maternal immune tolerance. Nuclear factor kappa B (NF-κB) pathway participates in regulating both innate and adaptive immunities, and lymph nodes play key roles in adaptive immune reaction. However, it is unclear whether early pregnancy changes the expression of NF-κB family in maternal lymph node in sheep. METHODS: In this study, the samples of inguinal lymph nodes were collected from ewes on day 16 of the estrous cycle, and on days 13, 16 and 25 of pregnancy, and expression of NF-κB family, including NF-κB p105 (NFKB1), NF-κB p100 (NFKB2), p65 (RELA), RelB (RELB) and c-Rel (REL), were analyzed through real-time quantitative PCR, Western blot and immunohistochemical analysis. RESULTS: The expression levels of NF-κB p105 and c-Rel downregulated, but NF-κB p100 upregulated on day 25 of pregnancy. The expression levels of p65, RelB and c-Rel peaked at day 13 of pregnancy, and expression level of RelB was higher during early pregnancy comparing to day 16 of the estrous cycle. In addition, p65 protein was located in the subcapsular sinus and lymph sinuses. CONCLUSION: This paper reported for the first time that early pregnancy has effects on the expression of NF-κB family, which may contribute to the maternal immunoregulation through blood circulation and lymph circulation during early pregnancy in sheep. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03373-7. BioMed Central 2022-07-11 /pmc/articles/PMC9275262/ /pubmed/35821130 http://dx.doi.org/10.1186/s12917-022-03373-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhang, Leying Zhang, Taipeng Yang, Zhen Cai, Chunjiang Hao, Shaopeng Yang, Ling Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy |
title | Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy |
title_full | Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy |
title_fullStr | Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy |
title_full_unstemmed | Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy |
title_short | Expression of nuclear factor kappa B in ovine maternal inguinal lymph nodes during early pregnancy |
title_sort | expression of nuclear factor kappa b in ovine maternal inguinal lymph nodes during early pregnancy |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275262/ https://www.ncbi.nlm.nih.gov/pubmed/35821130 http://dx.doi.org/10.1186/s12917-022-03373-7 |
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