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Long non-coding RNA MIR22HG suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microRNA-9-3p

The present study was designed to discuss long non-coding RNA (lncRNA) MIR22HG expression in prostate cancer and to address its effect on prostate cancer cells. MIR22HG and microRNA (miR)-9-3p expressions in prostate cancer cells were examined with the use of quantitative real-time PCR (qRT-PCR). Ce...

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Autores principales: Zhang, Wei, Shi, Chunmei, Xu, Qian, Chen, Xinfeng, Zhu, Hua, Zheng, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275890/
https://www.ncbi.nlm.nih.gov/pubmed/35611601
http://dx.doi.org/10.1080/21655979.2022.2079244
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author Zhang, Wei
Shi, Chunmei
Xu, Qian
Chen, Xinfeng
Zhu, Hua
Zheng, Bing
author_facet Zhang, Wei
Shi, Chunmei
Xu, Qian
Chen, Xinfeng
Zhu, Hua
Zheng, Bing
author_sort Zhang, Wei
collection PubMed
description The present study was designed to discuss long non-coding RNA (lncRNA) MIR22HG expression in prostate cancer and to address its effect on prostate cancer cells. MIR22HG and microRNA (miR)-9-3p expressions in prostate cancer cells were examined with the use of quantitative real-time PCR (qRT-PCR). Cell counting kit (CCK)-8, colony formation, and TUNEL were conducted to determine cell viability and apoptosis. Immunofluorescence was employed for the detection of Ki67 expression, and western blotting was applied for the examination of apoptosis-related proteins. The relationship of MIR22HG and miR-9-3p was verified employing luciferase reporter assay. Indeed, low MIR22HG expression was discovered in prostate cancer cells. Subsequently, in vitro loss-of-function studies revealed that MIR22HG overexpression suppressed cell proliferation but promoted cell apoptosis, accompanied with a reduction in Ki67 and Bcl-2 expressions, as well as an elevation in Bax and cleaved caspase 3 expressions. In addition, MIR22HG was identified as a sponge of miR-9-3p and the impacts of MIR22HG overexpression on cell proliferation and apoptosis were partly hindered by miR-9-3p overexpression. In summary, MIR22HG acts as an anticancer gene in prostate cancer via inhibiting cell proliferation and promoting apoptosis by sponging miR-9-3p. This article may provide a novel insight into the treatment of prostate cancer.
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spelling pubmed-92758902022-07-13 Long non-coding RNA MIR22HG suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microRNA-9-3p Zhang, Wei Shi, Chunmei Xu, Qian Chen, Xinfeng Zhu, Hua Zheng, Bing Bioengineered Research Paper The present study was designed to discuss long non-coding RNA (lncRNA) MIR22HG expression in prostate cancer and to address its effect on prostate cancer cells. MIR22HG and microRNA (miR)-9-3p expressions in prostate cancer cells were examined with the use of quantitative real-time PCR (qRT-PCR). Cell counting kit (CCK)-8, colony formation, and TUNEL were conducted to determine cell viability and apoptosis. Immunofluorescence was employed for the detection of Ki67 expression, and western blotting was applied for the examination of apoptosis-related proteins. The relationship of MIR22HG and miR-9-3p was verified employing luciferase reporter assay. Indeed, low MIR22HG expression was discovered in prostate cancer cells. Subsequently, in vitro loss-of-function studies revealed that MIR22HG overexpression suppressed cell proliferation but promoted cell apoptosis, accompanied with a reduction in Ki67 and Bcl-2 expressions, as well as an elevation in Bax and cleaved caspase 3 expressions. In addition, MIR22HG was identified as a sponge of miR-9-3p and the impacts of MIR22HG overexpression on cell proliferation and apoptosis were partly hindered by miR-9-3p overexpression. In summary, MIR22HG acts as an anticancer gene in prostate cancer via inhibiting cell proliferation and promoting apoptosis by sponging miR-9-3p. This article may provide a novel insight into the treatment of prostate cancer. Taylor & Francis 2022-05-25 /pmc/articles/PMC9275890/ /pubmed/35611601 http://dx.doi.org/10.1080/21655979.2022.2079244 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Zhang, Wei
Shi, Chunmei
Xu, Qian
Chen, Xinfeng
Zhu, Hua
Zheng, Bing
Long non-coding RNA MIR22HG suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microRNA-9-3p
title Long non-coding RNA MIR22HG suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microRNA-9-3p
title_full Long non-coding RNA MIR22HG suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microRNA-9-3p
title_fullStr Long non-coding RNA MIR22HG suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microRNA-9-3p
title_full_unstemmed Long non-coding RNA MIR22HG suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microRNA-9-3p
title_short Long non-coding RNA MIR22HG suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microRNA-9-3p
title_sort long non-coding rna mir22hg suppresses cell proliferation and promotes apoptosis in prostate cancer cells by sponging microrna-9-3p
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275890/
https://www.ncbi.nlm.nih.gov/pubmed/35611601
http://dx.doi.org/10.1080/21655979.2022.2079244
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