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MicroRNA-122-3p plays as the target of long non-coding RNA LINC00665 in repressing the progress of arthritis

MicroRNAs (miRNAs) play important roles in many diseases, including rheumatoid arthritis (RA). However, the mechanisms underlying the effects of miR-122-3p-3p on RA are not distinct and require further investigation. Patients with RA and healthy controls were recruited to analyze the miR-122-3p leve...

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Autores principales: Wang, Zhiyan, Tian, Qijun, Tian, Yumei, Zheng, Zhonghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275898/
https://www.ncbi.nlm.nih.gov/pubmed/35635065
http://dx.doi.org/10.1080/21655979.2022.2081757
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author Wang, Zhiyan
Tian, Qijun
Tian, Yumei
Zheng, Zhonghua
author_facet Wang, Zhiyan
Tian, Qijun
Tian, Yumei
Zheng, Zhonghua
author_sort Wang, Zhiyan
collection PubMed
description MicroRNAs (miRNAs) play important roles in many diseases, including rheumatoid arthritis (RA). However, the mechanisms underlying the effects of miR-122-3p-3p on RA are not distinct and require further investigation. Patients with RA and healthy controls were recruited to analyze the miR-122-3p levels. The MH7A cells were stimulated with interleukin (IL)-1β to mimic the local inflammation of RA. Cell Counting Kit-8 (CCK-8) and flow cytometry were performed to measure the viability and apoptosis of MH7A cells. Diana tools and TargetScan were used to predict the target relationships. Luciferase reporter assay was used to validate the target relationship. miR-122-3p is downregulated in RA patients and IL-1β-stimulated MH7A cells. miR-122-3p suppresses MH7A cell viability and promotes MH7A cell apoptosis. miR-122-3p targets LINC00665. LINC00665 eliminates the inhibitory effect of miR-122-3p on IL-1β-stimulated MH7A cells. Eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1) targets miR-122-3p. In addition, EIF2AK1 is highly expressed in patients with RA. In addition, EIF2AK1 activates the mTOR signaling pathway. miR-122-3p represses RA progression by reducing cell viability and increasing synoviocyte apoptosis.
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spelling pubmed-92758982022-07-13 MicroRNA-122-3p plays as the target of long non-coding RNA LINC00665 in repressing the progress of arthritis Wang, Zhiyan Tian, Qijun Tian, Yumei Zheng, Zhonghua Bioengineered Research Paper MicroRNAs (miRNAs) play important roles in many diseases, including rheumatoid arthritis (RA). However, the mechanisms underlying the effects of miR-122-3p-3p on RA are not distinct and require further investigation. Patients with RA and healthy controls were recruited to analyze the miR-122-3p levels. The MH7A cells were stimulated with interleukin (IL)-1β to mimic the local inflammation of RA. Cell Counting Kit-8 (CCK-8) and flow cytometry were performed to measure the viability and apoptosis of MH7A cells. Diana tools and TargetScan were used to predict the target relationships. Luciferase reporter assay was used to validate the target relationship. miR-122-3p is downregulated in RA patients and IL-1β-stimulated MH7A cells. miR-122-3p suppresses MH7A cell viability and promotes MH7A cell apoptosis. miR-122-3p targets LINC00665. LINC00665 eliminates the inhibitory effect of miR-122-3p on IL-1β-stimulated MH7A cells. Eukaryotic translation initiation factor 2 alpha kinase 1 (EIF2AK1) targets miR-122-3p. In addition, EIF2AK1 is highly expressed in patients with RA. In addition, EIF2AK1 activates the mTOR signaling pathway. miR-122-3p represses RA progression by reducing cell viability and increasing synoviocyte apoptosis. Taylor & Francis 2022-05-29 /pmc/articles/PMC9275898/ /pubmed/35635065 http://dx.doi.org/10.1080/21655979.2022.2081757 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Wang, Zhiyan
Tian, Qijun
Tian, Yumei
Zheng, Zhonghua
MicroRNA-122-3p plays as the target of long non-coding RNA LINC00665 in repressing the progress of arthritis
title MicroRNA-122-3p plays as the target of long non-coding RNA LINC00665 in repressing the progress of arthritis
title_full MicroRNA-122-3p plays as the target of long non-coding RNA LINC00665 in repressing the progress of arthritis
title_fullStr MicroRNA-122-3p plays as the target of long non-coding RNA LINC00665 in repressing the progress of arthritis
title_full_unstemmed MicroRNA-122-3p plays as the target of long non-coding RNA LINC00665 in repressing the progress of arthritis
title_short MicroRNA-122-3p plays as the target of long non-coding RNA LINC00665 in repressing the progress of arthritis
title_sort microrna-122-3p plays as the target of long non-coding rna linc00665 in repressing the progress of arthritis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275898/
https://www.ncbi.nlm.nih.gov/pubmed/35635065
http://dx.doi.org/10.1080/21655979.2022.2081757
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