Cargando…

The functional activity of donor kidneys is negatively regulated by microribonucleic acid-451 in different perfusion methods to inhibit adenosine triphosphate metabolism and the proliferation of HK2 cells

This study explored the regulation of different perfusion methods on ischemia-reperfusion injury in donor kidneys. In this study, renal cortical/medullary tissue specimens were collected from porcine kidneys donors using different perfusion methods at various time points. Hematoxylin and eosin (H&am...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Xu-Hui, Han, Long-Xi, Zhang, Rong-Jie, Zhang, Peng, Chen, Fu-Gang, Yu, Jia, Luo, Heng, Han, Xiu-Wu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275911/
https://www.ncbi.nlm.nih.gov/pubmed/35603466
http://dx.doi.org/10.1080/21655979.2022.2068739
_version_ 1784745593721061376
author Zhu, Xu-Hui
Han, Long-Xi
Zhang, Rong-Jie
Zhang, Peng
Chen, Fu-Gang
Yu, Jia
Luo, Heng
Han, Xiu-Wu
author_facet Zhu, Xu-Hui
Han, Long-Xi
Zhang, Rong-Jie
Zhang, Peng
Chen, Fu-Gang
Yu, Jia
Luo, Heng
Han, Xiu-Wu
author_sort Zhu, Xu-Hui
collection PubMed
description This study explored the regulation of different perfusion methods on ischemia-reperfusion injury in donor kidneys. In this study, renal cortical/medullary tissue specimens were collected from porcine kidneys donors using different perfusion methods at various time points. Hematoxylin and eosin (H&E) staining was used to test the histological differences. Differentially expressed micro-ribonucleic acids (miRNAs) were identified by miRNA transcriptome sequencing. Reverse transcription-polymerase chain reaction (RT-PCR) tests were used to verify the changes in miRNAs in the kidney tissue taken from different perfusion groups. The related signaling pathways and the changes in the cell functions of different perfusion groups were analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) /Gene Ontology (GO) bioinformatics analyses. The effects of miRNA overexpression on the metabolism and proliferation of HK2 cells were detected by ATP kit and MTT assay. The H&E staining results showed that there were essentially no differences in the tissue samples among different perfusion groups at and before 12 h compared with a control group. The quantitative PCR results revealed that there was essentially no change in the expression of ssc-miR-451, ssc-miR-1285, and ssc-miR-486 in the cis infusion or joint infusion kidney groups, and their expression was significantly down-regulated over time in the trans-infusion kidney group. The bioinformatics analysis showed that the cellular component, molecular function, and biological processes of the kidney tissue, which had been perfused using three methods, had been consistently affected. The most significant changes after perfusion occurred in the intracellular metabolism signaling pathways. Furthermore, the energy metabolism and proliferation of the HK2 cells were significantly inhibited after the overexpression of miR-451. Specific miRNA markers, such as miR-451, may play a negative regulatory role in cell metabolism following the perfusion of kidney transplants using different methods.
format Online
Article
Text
id pubmed-9275911
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-92759112022-07-13 The functional activity of donor kidneys is negatively regulated by microribonucleic acid-451 in different perfusion methods to inhibit adenosine triphosphate metabolism and the proliferation of HK2 cells Zhu, Xu-Hui Han, Long-Xi Zhang, Rong-Jie Zhang, Peng Chen, Fu-Gang Yu, Jia Luo, Heng Han, Xiu-Wu Bioengineered Research Paper This study explored the regulation of different perfusion methods on ischemia-reperfusion injury in donor kidneys. In this study, renal cortical/medullary tissue specimens were collected from porcine kidneys donors using different perfusion methods at various time points. Hematoxylin and eosin (H&E) staining was used to test the histological differences. Differentially expressed micro-ribonucleic acids (miRNAs) were identified by miRNA transcriptome sequencing. Reverse transcription-polymerase chain reaction (RT-PCR) tests were used to verify the changes in miRNAs in the kidney tissue taken from different perfusion groups. The related signaling pathways and the changes in the cell functions of different perfusion groups were analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) /Gene Ontology (GO) bioinformatics analyses. The effects of miRNA overexpression on the metabolism and proliferation of HK2 cells were detected by ATP kit and MTT assay. The H&E staining results showed that there were essentially no differences in the tissue samples among different perfusion groups at and before 12 h compared with a control group. The quantitative PCR results revealed that there was essentially no change in the expression of ssc-miR-451, ssc-miR-1285, and ssc-miR-486 in the cis infusion or joint infusion kidney groups, and their expression was significantly down-regulated over time in the trans-infusion kidney group. The bioinformatics analysis showed that the cellular component, molecular function, and biological processes of the kidney tissue, which had been perfused using three methods, had been consistently affected. The most significant changes after perfusion occurred in the intracellular metabolism signaling pathways. Furthermore, the energy metabolism and proliferation of the HK2 cells were significantly inhibited after the overexpression of miR-451. Specific miRNA markers, such as miR-451, may play a negative regulatory role in cell metabolism following the perfusion of kidney transplants using different methods. Taylor & Francis 2022-05-21 /pmc/articles/PMC9275911/ /pubmed/35603466 http://dx.doi.org/10.1080/21655979.2022.2068739 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Zhu, Xu-Hui
Han, Long-Xi
Zhang, Rong-Jie
Zhang, Peng
Chen, Fu-Gang
Yu, Jia
Luo, Heng
Han, Xiu-Wu
The functional activity of donor kidneys is negatively regulated by microribonucleic acid-451 in different perfusion methods to inhibit adenosine triphosphate metabolism and the proliferation of HK2 cells
title The functional activity of donor kidneys is negatively regulated by microribonucleic acid-451 in different perfusion methods to inhibit adenosine triphosphate metabolism and the proliferation of HK2 cells
title_full The functional activity of donor kidneys is negatively regulated by microribonucleic acid-451 in different perfusion methods to inhibit adenosine triphosphate metabolism and the proliferation of HK2 cells
title_fullStr The functional activity of donor kidneys is negatively regulated by microribonucleic acid-451 in different perfusion methods to inhibit adenosine triphosphate metabolism and the proliferation of HK2 cells
title_full_unstemmed The functional activity of donor kidneys is negatively regulated by microribonucleic acid-451 in different perfusion methods to inhibit adenosine triphosphate metabolism and the proliferation of HK2 cells
title_short The functional activity of donor kidneys is negatively regulated by microribonucleic acid-451 in different perfusion methods to inhibit adenosine triphosphate metabolism and the proliferation of HK2 cells
title_sort functional activity of donor kidneys is negatively regulated by microribonucleic acid-451 in different perfusion methods to inhibit adenosine triphosphate metabolism and the proliferation of hk2 cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275911/
https://www.ncbi.nlm.nih.gov/pubmed/35603466
http://dx.doi.org/10.1080/21655979.2022.2068739
work_keys_str_mv AT zhuxuhui thefunctionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT hanlongxi thefunctionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT zhangrongjie thefunctionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT zhangpeng thefunctionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT chenfugang thefunctionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT yujia thefunctionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT luoheng thefunctionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT hanxiuwu thefunctionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT zhuxuhui functionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT hanlongxi functionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT zhangrongjie functionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT zhangpeng functionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT chenfugang functionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT yujia functionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT luoheng functionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells
AT hanxiuwu functionalactivityofdonorkidneysisnegativelyregulatedbymicroribonucleicacid451indifferentperfusionmethodstoinhibitadenosinetriphosphatemetabolismandtheproliferationofhk2cells