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Different responses of cervical intervertebral disc caused by low and high virulence bacterial infection: a comparative study in rats

The aims of this study were to investigate the outcomes of low- and high-virulence bacterial cervical intervertebral discs (IVDs) infection and its association with cervical IVDs degeneration in rats. A total of 75 clean grade male rats were used to establish the corresponding animal models of low a...

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Autores principales: Li, Jie, Chen, Yilei, Wu, Hao, Shan, Zhi, Bei, Dikai, Gan, Kaifeng, Liu, Junhui, Zhang, Xuyang, Chen, Binhui, Chen, Jian, Zhao, Feng-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275948/
https://www.ncbi.nlm.nih.gov/pubmed/35587595
http://dx.doi.org/10.1080/21655979.2022.2075305
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author Li, Jie
Chen, Yilei
Wu, Hao
Shan, Zhi
Bei, Dikai
Gan, Kaifeng
Liu, Junhui
Zhang, Xuyang
Chen, Binhui
Chen, Jian
Zhao, Feng-Dong
author_facet Li, Jie
Chen, Yilei
Wu, Hao
Shan, Zhi
Bei, Dikai
Gan, Kaifeng
Liu, Junhui
Zhang, Xuyang
Chen, Binhui
Chen, Jian
Zhao, Feng-Dong
author_sort Li, Jie
collection PubMed
description The aims of this study were to investigate the outcomes of low- and high-virulence bacterial cervical intervertebral discs (IVDs) infection and its association with cervical IVDs degeneration in rats. A total of 75 clean grade male rats were used to establish the corresponding animal models of low and high virulent bacterial cervical disc infection via an anterior cervical approach, with injection of Propionibacterium acnes (P. acnes) and Staphylococcus epidermidis (S. epidermidis) with a 29 G needle to cervical IVDs. Specimens were collected for evaluation of Blood routine (Blood-RT), histological staining, and gene expression assays after a magnetic resonance imaging (MRI) scan. There were no statistical differences in all groups in white blood cells (WBC) at 2 and 6 weeks postoperatively (P = 0.136). The highest percentage of neutrophils was found in the S. epidermidis group at 2 weeks postoperatively (P = 0.043). MRI and histology showed that at 6 weeks postoperatively, the puncture group and P. acnes group had similar disc degeneration. In the S. epidermidis group, the disc and subchondral bone structure had been destroyed and bony fusion had occurred after the discitis. The upregulation of pro-inflammatory factor expression had the strongest effect of S. epidermidis on the early stage, while the upregulation in the puncture and P. acnes groups was more persistent. P. acnes infection of the cervical IVDs can lead to degenerative changes, whereas S. epidermidis infection leads to the manifestation of septic discitis. The correlation between P. acnes infection and cervical IVDs degeneration found in clinical studies was confirmed.
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spelling pubmed-92759482022-07-13 Different responses of cervical intervertebral disc caused by low and high virulence bacterial infection: a comparative study in rats Li, Jie Chen, Yilei Wu, Hao Shan, Zhi Bei, Dikai Gan, Kaifeng Liu, Junhui Zhang, Xuyang Chen, Binhui Chen, Jian Zhao, Feng-Dong Bioengineered Research Paper The aims of this study were to investigate the outcomes of low- and high-virulence bacterial cervical intervertebral discs (IVDs) infection and its association with cervical IVDs degeneration in rats. A total of 75 clean grade male rats were used to establish the corresponding animal models of low and high virulent bacterial cervical disc infection via an anterior cervical approach, with injection of Propionibacterium acnes (P. acnes) and Staphylococcus epidermidis (S. epidermidis) with a 29 G needle to cervical IVDs. Specimens were collected for evaluation of Blood routine (Blood-RT), histological staining, and gene expression assays after a magnetic resonance imaging (MRI) scan. There were no statistical differences in all groups in white blood cells (WBC) at 2 and 6 weeks postoperatively (P = 0.136). The highest percentage of neutrophils was found in the S. epidermidis group at 2 weeks postoperatively (P = 0.043). MRI and histology showed that at 6 weeks postoperatively, the puncture group and P. acnes group had similar disc degeneration. In the S. epidermidis group, the disc and subchondral bone structure had been destroyed and bony fusion had occurred after the discitis. The upregulation of pro-inflammatory factor expression had the strongest effect of S. epidermidis on the early stage, while the upregulation in the puncture and P. acnes groups was more persistent. P. acnes infection of the cervical IVDs can lead to degenerative changes, whereas S. epidermidis infection leads to the manifestation of septic discitis. The correlation between P. acnes infection and cervical IVDs degeneration found in clinical studies was confirmed. Taylor & Francis 2022-05-19 /pmc/articles/PMC9275948/ /pubmed/35587595 http://dx.doi.org/10.1080/21655979.2022.2075305 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Li, Jie
Chen, Yilei
Wu, Hao
Shan, Zhi
Bei, Dikai
Gan, Kaifeng
Liu, Junhui
Zhang, Xuyang
Chen, Binhui
Chen, Jian
Zhao, Feng-Dong
Different responses of cervical intervertebral disc caused by low and high virulence bacterial infection: a comparative study in rats
title Different responses of cervical intervertebral disc caused by low and high virulence bacterial infection: a comparative study in rats
title_full Different responses of cervical intervertebral disc caused by low and high virulence bacterial infection: a comparative study in rats
title_fullStr Different responses of cervical intervertebral disc caused by low and high virulence bacterial infection: a comparative study in rats
title_full_unstemmed Different responses of cervical intervertebral disc caused by low and high virulence bacterial infection: a comparative study in rats
title_short Different responses of cervical intervertebral disc caused by low and high virulence bacterial infection: a comparative study in rats
title_sort different responses of cervical intervertebral disc caused by low and high virulence bacterial infection: a comparative study in rats
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275948/
https://www.ncbi.nlm.nih.gov/pubmed/35587595
http://dx.doi.org/10.1080/21655979.2022.2075305
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