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Interleukin 35 regulates interleukin 17 expression and T helper 17 in patients with proliferative diabetic retinopathy
T helper 17 (Th17) cells regulate inflammatory processes and are implicated in pathogenesis of proliferative diabetic retinopathy (PDR) through modulation of interleukin-17 (IL-17). IL-35, anti-inflammatory factor, negatively mediates IL-17 expression and Th17 differentiation. In this study, the rol...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275983/ https://www.ncbi.nlm.nih.gov/pubmed/35635032 http://dx.doi.org/10.1080/21655979.2022.2080367 |
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author | Yan, Ai Zhang, Ying Wang, Xiaocong Cui, Yueling Tan, Wei |
author_facet | Yan, Ai Zhang, Ying Wang, Xiaocong Cui, Yueling Tan, Wei |
author_sort | Yan, Ai |
collection | PubMed |
description | T helper 17 (Th17) cells regulate inflammatory processes and are implicated in pathogenesis of proliferative diabetic retinopathy (PDR) through modulation of interleukin-17 (IL-17). IL-35, anti-inflammatory factor, negatively mediates IL-17 expression and Th17 differentiation. In this study, the role of IL-35 in PDR was assessed. The results showed that IL-35 was down-regulated, while IL-17 was up-regulated, in peripheral blood mononuclear cells (PBMCs) of PDR patients. In addition, immunofluorescence analysis indicated that frequency of Th17 cells was enhanced in the PBMCs of PDR patients. However, incubation with IL-35 reduced the Th17 cell frequency and decreased the level of IL-17 in CD4(+) T lymphocytes. Moreover, the levels of transcription factors essential for Th17 differentiation, ROR α (retinoid-related orphan receptor alpha) and ROR γt, were reduced by IL-35 treatment. In conclusion, IL-35 reduced level of IL-17 and inhibited Th17 differentiation to protect against PDR. |
format | Online Article Text |
id | pubmed-9275983 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-92759832022-07-13 Interleukin 35 regulates interleukin 17 expression and T helper 17 in patients with proliferative diabetic retinopathy Yan, Ai Zhang, Ying Wang, Xiaocong Cui, Yueling Tan, Wei Bioengineered Research Paper T helper 17 (Th17) cells regulate inflammatory processes and are implicated in pathogenesis of proliferative diabetic retinopathy (PDR) through modulation of interleukin-17 (IL-17). IL-35, anti-inflammatory factor, negatively mediates IL-17 expression and Th17 differentiation. In this study, the role of IL-35 in PDR was assessed. The results showed that IL-35 was down-regulated, while IL-17 was up-regulated, in peripheral blood mononuclear cells (PBMCs) of PDR patients. In addition, immunofluorescence analysis indicated that frequency of Th17 cells was enhanced in the PBMCs of PDR patients. However, incubation with IL-35 reduced the Th17 cell frequency and decreased the level of IL-17 in CD4(+) T lymphocytes. Moreover, the levels of transcription factors essential for Th17 differentiation, ROR α (retinoid-related orphan receptor alpha) and ROR γt, were reduced by IL-35 treatment. In conclusion, IL-35 reduced level of IL-17 and inhibited Th17 differentiation to protect against PDR. Taylor & Francis 2022-05-29 /pmc/articles/PMC9275983/ /pubmed/35635032 http://dx.doi.org/10.1080/21655979.2022.2080367 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Yan, Ai Zhang, Ying Wang, Xiaocong Cui, Yueling Tan, Wei Interleukin 35 regulates interleukin 17 expression and T helper 17 in patients with proliferative diabetic retinopathy |
title | Interleukin 35 regulates interleukin 17 expression and T helper 17 in patients with proliferative diabetic retinopathy |
title_full | Interleukin 35 regulates interleukin 17 expression and T helper 17 in patients with proliferative diabetic retinopathy |
title_fullStr | Interleukin 35 regulates interleukin 17 expression and T helper 17 in patients with proliferative diabetic retinopathy |
title_full_unstemmed | Interleukin 35 regulates interleukin 17 expression and T helper 17 in patients with proliferative diabetic retinopathy |
title_short | Interleukin 35 regulates interleukin 17 expression and T helper 17 in patients with proliferative diabetic retinopathy |
title_sort | interleukin 35 regulates interleukin 17 expression and t helper 17 in patients with proliferative diabetic retinopathy |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275983/ https://www.ncbi.nlm.nih.gov/pubmed/35635032 http://dx.doi.org/10.1080/21655979.2022.2080367 |
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