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Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp

Information on the neurons and axons that express the mechanosensitive channel Piezo1 and its expression in axons innervating the dental pulp may help understand the nature of the Piezo1-mediated mechanosensation and the underlying mechanism of dentin sensitivity elicited by mechanical stimuli. For...

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Autores principales: Cho, Yi Sul, Han, Hye Min, Jeong, Soon Youn, Kim, Tae Heon, Choi, So Young, Kim, Yun Sook, Bae, Yong Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276962/
https://www.ncbi.nlm.nih.gov/pubmed/35846568
http://dx.doi.org/10.3389/fncel.2022.945948
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author Cho, Yi Sul
Han, Hye Min
Jeong, Soon Youn
Kim, Tae Heon
Choi, So Young
Kim, Yun Sook
Bae, Yong Chul
author_facet Cho, Yi Sul
Han, Hye Min
Jeong, Soon Youn
Kim, Tae Heon
Choi, So Young
Kim, Yun Sook
Bae, Yong Chul
author_sort Cho, Yi Sul
collection PubMed
description Information on the neurons and axons that express the mechanosensitive channel Piezo1 and its expression in axons innervating the dental pulp may help understand the nature of the Piezo1-mediated mechanosensation and the underlying mechanism of dentin sensitivity elicited by mechanical stimuli. For this, we here investigated the neurochemical properties of the neurons in the rat trigeminal ganglion (TG) and their axons in its sensory root that express Piezo1 and the expression of Piezo1 in the rat and human dental pulp by light and electron microscopic immunohistochemistry and quantitative analysis. Piezo1 was expressed mainly in medium-sized and large TG neurons. Piezo1-immunopositive (+) neurons frequently coexpressed the marker for neurons with myelinated axons, NF200, but rarely the markers for neurons with unmyelinated axons, CGRP or IB4. In the sensory root of TG, Piezo1 was expressed primarily in small myelinated axons (Aδ, 60.2%) but also in large myelinated (Aβ, 24.3%) and unmyelinated (C, 15.5%) axons. In the human dental pulp, Piezo1 was expressed in numerous NF200+ axons, which formed a network in the peripheral pulp and often “ascended” toward the dentin. Most Piezo1+ myelinated axons in the radicular pulp became unmyelinated in the peripheral pulp, where Piezo1 immunoreaction product was associated with the axonal plasma membrane, suggesting a functional role of Piezo1 in the peripheral pulp. These findings suggest that Piezo1 is involved primarily in mediating the acute pain elicited by high-threshold mechanical stimuli, and that the Piezo1-mediated dental mechanotransduction occurs primarily in the axons in the peripheral pulp.
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spelling pubmed-92769622022-07-14 Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp Cho, Yi Sul Han, Hye Min Jeong, Soon Youn Kim, Tae Heon Choi, So Young Kim, Yun Sook Bae, Yong Chul Front Cell Neurosci Cellular Neuroscience Information on the neurons and axons that express the mechanosensitive channel Piezo1 and its expression in axons innervating the dental pulp may help understand the nature of the Piezo1-mediated mechanosensation and the underlying mechanism of dentin sensitivity elicited by mechanical stimuli. For this, we here investigated the neurochemical properties of the neurons in the rat trigeminal ganglion (TG) and their axons in its sensory root that express Piezo1 and the expression of Piezo1 in the rat and human dental pulp by light and electron microscopic immunohistochemistry and quantitative analysis. Piezo1 was expressed mainly in medium-sized and large TG neurons. Piezo1-immunopositive (+) neurons frequently coexpressed the marker for neurons with myelinated axons, NF200, but rarely the markers for neurons with unmyelinated axons, CGRP or IB4. In the sensory root of TG, Piezo1 was expressed primarily in small myelinated axons (Aδ, 60.2%) but also in large myelinated (Aβ, 24.3%) and unmyelinated (C, 15.5%) axons. In the human dental pulp, Piezo1 was expressed in numerous NF200+ axons, which formed a network in the peripheral pulp and often “ascended” toward the dentin. Most Piezo1+ myelinated axons in the radicular pulp became unmyelinated in the peripheral pulp, where Piezo1 immunoreaction product was associated with the axonal plasma membrane, suggesting a functional role of Piezo1 in the peripheral pulp. These findings suggest that Piezo1 is involved primarily in mediating the acute pain elicited by high-threshold mechanical stimuli, and that the Piezo1-mediated dental mechanotransduction occurs primarily in the axons in the peripheral pulp. Frontiers Media S.A. 2022-06-29 /pmc/articles/PMC9276962/ /pubmed/35846568 http://dx.doi.org/10.3389/fncel.2022.945948 Text en Copyright © 2022 Cho, Han, Jeong, Kim, Choi, Kim and Bae. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular Neuroscience
Cho, Yi Sul
Han, Hye Min
Jeong, Soon Youn
Kim, Tae Heon
Choi, So Young
Kim, Yun Sook
Bae, Yong Chul
Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp
title Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp
title_full Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp
title_fullStr Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp
title_full_unstemmed Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp
title_short Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp
title_sort expression of piezo1 in the trigeminal neurons and in the axons that innervate the dental pulp
topic Cellular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276962/
https://www.ncbi.nlm.nih.gov/pubmed/35846568
http://dx.doi.org/10.3389/fncel.2022.945948
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