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High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats

OBJECTIVE: A role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats. METHODS: Eighteen male Wis...

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Autores principales: Delfan, Maryam, Amadeh Juybari, Raheleh, Gorgani-Firuzjaee, Sattar, Høiriis Nielsen, Jens, Delfan, Neda, Laher, Ismail, Saeidi, Ayoub, Granacher, Urs, Zouhal, Hassane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277013/
https://www.ncbi.nlm.nih.gov/pubmed/35845054
http://dx.doi.org/10.3389/fcvm.2022.927956
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author Delfan, Maryam
Amadeh Juybari, Raheleh
Gorgani-Firuzjaee, Sattar
Høiriis Nielsen, Jens
Delfan, Neda
Laher, Ismail
Saeidi, Ayoub
Granacher, Urs
Zouhal, Hassane
author_facet Delfan, Maryam
Amadeh Juybari, Raheleh
Gorgani-Firuzjaee, Sattar
Høiriis Nielsen, Jens
Delfan, Neda
Laher, Ismail
Saeidi, Ayoub
Granacher, Urs
Zouhal, Hassane
author_sort Delfan, Maryam
collection PubMed
description OBJECTIVE: A role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats. METHODS: Eighteen male Wistar rats (260 ± 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions. RESULTS: Both exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, η(2): 0.909). While the expressions of miR-206 and apoptotic markers decreased in both training protocols (p < 0.001, η(2): 0.967), HIIT caused greater reductions in apoptotic markers and produced a 20% greater reduction in miR-206 compared with the MICT protocol (p < 0.001). Furthermore, both training protocols enhanced the expression of HSP60 (p < 0.001, η(2): 0.976), with a nearly 50% greater increase in the HIIT group compared with MICT. CONCLUSIONS: Our results indicate that both exercise protocols, HIIT and MICT, have the potential to reduce diabetic cardiomyopathy by modifying the expression of miR-206 and its downstream targets of apoptosis. It seems however that HIIT is even more effective than MICT to modulate these molecular markers.
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spelling pubmed-92770132022-07-14 High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats Delfan, Maryam Amadeh Juybari, Raheleh Gorgani-Firuzjaee, Sattar Høiriis Nielsen, Jens Delfan, Neda Laher, Ismail Saeidi, Ayoub Granacher, Urs Zouhal, Hassane Front Cardiovasc Med Cardiovascular Medicine OBJECTIVE: A role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats. METHODS: Eighteen male Wistar rats (260 ± 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions. RESULTS: Both exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, η(2): 0.909). While the expressions of miR-206 and apoptotic markers decreased in both training protocols (p < 0.001, η(2): 0.967), HIIT caused greater reductions in apoptotic markers and produced a 20% greater reduction in miR-206 compared with the MICT protocol (p < 0.001). Furthermore, both training protocols enhanced the expression of HSP60 (p < 0.001, η(2): 0.976), with a nearly 50% greater increase in the HIIT group compared with MICT. CONCLUSIONS: Our results indicate that both exercise protocols, HIIT and MICT, have the potential to reduce diabetic cardiomyopathy by modifying the expression of miR-206 and its downstream targets of apoptosis. It seems however that HIIT is even more effective than MICT to modulate these molecular markers. Frontiers Media S.A. 2022-06-29 /pmc/articles/PMC9277013/ /pubmed/35845054 http://dx.doi.org/10.3389/fcvm.2022.927956 Text en Copyright © 2022 Delfan, Amadeh Juybari, Gorgani-Firuzjaee, Høiriis Nielsen, Delfan, Laher, Saeidi, Granacher and Zouhal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Delfan, Maryam
Amadeh Juybari, Raheleh
Gorgani-Firuzjaee, Sattar
Høiriis Nielsen, Jens
Delfan, Neda
Laher, Ismail
Saeidi, Ayoub
Granacher, Urs
Zouhal, Hassane
High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats
title High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats
title_full High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats
title_fullStr High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats
title_full_unstemmed High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats
title_short High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats
title_sort high-intensity interval training improves cardiac function by mir-206 dependent hsp60 induction in diabetic rats
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277013/
https://www.ncbi.nlm.nih.gov/pubmed/35845054
http://dx.doi.org/10.3389/fcvm.2022.927956
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