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Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography

Seed germination and uniform plant stand in the field are the most critical crop growth stages determining the final yield. Pea ( Pisum sativum L.) seeds production is often hampered due to the seed dormancy caused by the hard seed coat. Such effect is mainly attributed to poor or uneven germination...

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Detalles Bibliográficos
Autores principales: Li, Xinhua, Yang, Xingyu, Li, Xiaoran, Zhao, Zhiyi, Zhang, Zijian, Lin, Hungyen, Kang, Dingming, Shen, Yaochun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277031/
https://www.ncbi.nlm.nih.gov/pubmed/35844779
http://dx.doi.org/10.1002/pld3.428
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author Li, Xinhua
Yang, Xingyu
Li, Xiaoran
Zhao, Zhiyi
Zhang, Zijian
Lin, Hungyen
Kang, Dingming
Shen, Yaochun
author_facet Li, Xinhua
Yang, Xingyu
Li, Xiaoran
Zhao, Zhiyi
Zhang, Zijian
Lin, Hungyen
Kang, Dingming
Shen, Yaochun
author_sort Li, Xinhua
collection PubMed
description Seed germination and uniform plant stand in the field are the most critical crop growth stages determining the final yield. Pea ( Pisum sativum L.) seeds production is often hampered due to the seed dormancy caused by the hard seed coat. Such effect is mainly attributed to poor or uneven germination and unsynchronised seedling emergence. Understanding the time course of water intake and several critical germination indicators can reveal many features of seed germination such as rate and uniformity. This paper used optical coherence tomography (OCT), a noninvasive and cross‐sectional imaging technique, to monitor the inner structural changes throughout the germination process. A sequence of cross‐sectional OCT images of pea ( P. sativum L.) seeds, together with additional microscopic optical images, was recorded continuously and in situ for over 40 h. OCT and microscopic images revealed the changes in the internal structure and the external shape of the pea seeds during germination, respectively. It was found that the cross‐sectional OCT images helped to identify the critical indicators distinguishing the different phases of germination pea seeds. Therefore, the presented OCT approach offers a fast and nondestructive way to precisely measure the structural indicators in different germination phases.
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spelling pubmed-92770312022-07-15 Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography Li, Xinhua Yang, Xingyu Li, Xiaoran Zhao, Zhiyi Zhang, Zijian Lin, Hungyen Kang, Dingming Shen, Yaochun Plant Direct Original Research Seed germination and uniform plant stand in the field are the most critical crop growth stages determining the final yield. Pea ( Pisum sativum L.) seeds production is often hampered due to the seed dormancy caused by the hard seed coat. Such effect is mainly attributed to poor or uneven germination and unsynchronised seedling emergence. Understanding the time course of water intake and several critical germination indicators can reveal many features of seed germination such as rate and uniformity. This paper used optical coherence tomography (OCT), a noninvasive and cross‐sectional imaging technique, to monitor the inner structural changes throughout the germination process. A sequence of cross‐sectional OCT images of pea ( P. sativum L.) seeds, together with additional microscopic optical images, was recorded continuously and in situ for over 40 h. OCT and microscopic images revealed the changes in the internal structure and the external shape of the pea seeds during germination, respectively. It was found that the cross‐sectional OCT images helped to identify the critical indicators distinguishing the different phases of germination pea seeds. Therefore, the presented OCT approach offers a fast and nondestructive way to precisely measure the structural indicators in different germination phases. John Wiley and Sons Inc. 2022-07-12 /pmc/articles/PMC9277031/ /pubmed/35844779 http://dx.doi.org/10.1002/pld3.428 Text en © 2022 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Li, Xinhua
Yang, Xingyu
Li, Xiaoran
Zhao, Zhiyi
Zhang, Zijian
Lin, Hungyen
Kang, Dingming
Shen, Yaochun
Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography
title Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography
title_full Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography
title_fullStr Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography
title_full_unstemmed Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography
title_short Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography
title_sort nondestructive in situ monitoring of pea seeds germination using optical coherence tomography
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277031/
https://www.ncbi.nlm.nih.gov/pubmed/35844779
http://dx.doi.org/10.1002/pld3.428
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