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Ratiometric control of cell phenotypes in monostrain microbial consortia

We address the problem of regulating and keeping at a desired balance the relative numbers between cells exhibiting a different phenotype within a monostrain microbial consortium. We propose a strategy based on the use of external control inputs, assuming each cell in the community is endowed with a...

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Detalles Bibliográficos
Autores principales: Salzano, Davide, Fiore, Davide, di Bernardo, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277296/
https://www.ncbi.nlm.nih.gov/pubmed/35858050
http://dx.doi.org/10.1098/rsif.2022.0335
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author Salzano, Davide
Fiore, Davide
di Bernardo, Mario
author_facet Salzano, Davide
Fiore, Davide
di Bernardo, Mario
author_sort Salzano, Davide
collection PubMed
description We address the problem of regulating and keeping at a desired balance the relative numbers between cells exhibiting a different phenotype within a monostrain microbial consortium. We propose a strategy based on the use of external control inputs, assuming each cell in the community is endowed with a reversible, bistable memory mechanism. Specifically, we provide a general analytical framework to guide the design of external feedback control strategies aimed at balancing the ratio between cells whose memory is stabilized at either one of two equilibria associated with different cell phenotypes. We demonstrate the stability and robustness properties of the control laws proposed and validate them in silico, implementing the memory element via a genetic toggle-switch. The proposed control framework may be used to allow long-term coexistence of different populations, with both industrial and biotechnological applications. As a representative example, we consider the realistic agent-based implementation of our control strategy to enable cooperative bioproduction of a dimer in a monostrain microbial consortium.
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spelling pubmed-92772962022-10-19 Ratiometric control of cell phenotypes in monostrain microbial consortia Salzano, Davide Fiore, Davide di Bernardo, Mario J R Soc Interface Life Sciences–Engineering interface We address the problem of regulating and keeping at a desired balance the relative numbers between cells exhibiting a different phenotype within a monostrain microbial consortium. We propose a strategy based on the use of external control inputs, assuming each cell in the community is endowed with a reversible, bistable memory mechanism. Specifically, we provide a general analytical framework to guide the design of external feedback control strategies aimed at balancing the ratio between cells whose memory is stabilized at either one of two equilibria associated with different cell phenotypes. We demonstrate the stability and robustness properties of the control laws proposed and validate them in silico, implementing the memory element via a genetic toggle-switch. The proposed control framework may be used to allow long-term coexistence of different populations, with both industrial and biotechnological applications. As a representative example, we consider the realistic agent-based implementation of our control strategy to enable cooperative bioproduction of a dimer in a monostrain microbial consortium. The Royal Society 2022-07-13 /pmc/articles/PMC9277296/ /pubmed/35858050 http://dx.doi.org/10.1098/rsif.2022.0335 Text en © 2022 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Life Sciences–Engineering interface
Salzano, Davide
Fiore, Davide
di Bernardo, Mario
Ratiometric control of cell phenotypes in monostrain microbial consortia
title Ratiometric control of cell phenotypes in monostrain microbial consortia
title_full Ratiometric control of cell phenotypes in monostrain microbial consortia
title_fullStr Ratiometric control of cell phenotypes in monostrain microbial consortia
title_full_unstemmed Ratiometric control of cell phenotypes in monostrain microbial consortia
title_short Ratiometric control of cell phenotypes in monostrain microbial consortia
title_sort ratiometric control of cell phenotypes in monostrain microbial consortia
topic Life Sciences–Engineering interface
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277296/
https://www.ncbi.nlm.nih.gov/pubmed/35858050
http://dx.doi.org/10.1098/rsif.2022.0335
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