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Integrative Analysis of Genes Involved in the Global Response to Potato Wart Formation

Synchytrium endobioticum, the causal agent of potato wart disease, poses a major threat to commercial potato production. Understanding the roles of transcriptionally regulated genes following pathogen infection is necessary for understanding the system-level host response to pathogen. Although some...

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Autores principales: Yan, Lang, Li, Yan, Qing, Yuan, Tao, Xiang, Wang, Haiyan, Lai, Xianjun, Zhang, Yizheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277394/
https://www.ncbi.nlm.nih.gov/pubmed/35845669
http://dx.doi.org/10.3389/fpls.2022.865716
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author Yan, Lang
Li, Yan
Qing, Yuan
Tao, Xiang
Wang, Haiyan
Lai, Xianjun
Zhang, Yizheng
author_facet Yan, Lang
Li, Yan
Qing, Yuan
Tao, Xiang
Wang, Haiyan
Lai, Xianjun
Zhang, Yizheng
author_sort Yan, Lang
collection PubMed
description Synchytrium endobioticum, the causal agent of potato wart disease, poses a major threat to commercial potato production. Understanding the roles of transcriptionally regulated genes following pathogen infection is necessary for understanding the system-level host response to pathogen. Although some understanding of defense mechanisms against S. endobioticum infection has been gained for incompatible interactions, the genes and signaling pathways involved in the compatible interaction remain unclear. Based on the collection of wart diseased tubers of a susceptible cultivar, we performed phenotypic and dual RNA-Seq analyses of wart lesions in seven stages of disease progression. We totally detected 5,052 differentially expressed genes (DEGs) by comparing the different stages of infection to uninfected controls. The tendency toward differential gene expression was active rather than suppressed under attack by the pathogen. The number of DEGs step-up along with the development of the disease and the first, third and seventh of the disease stages showed substantially increase of DEGs in comparison of the previous stage. The important functional groups identified via Gene ontology (GO) and KEGG enrichment were those responsible for plant-pathogen interaction, fatty acid elongation and phenylpropanoid biosynthesis. Gene coexpression networks, composed of 17 distinct gene modules that contained between 25 and 813 genes, revealed high interconnectivity of the induced response and led to the identification of a number of hub genes enriched at different stages of infection. These results provide a comprehensive perspective on the global response of potato to S. endobioticum infection and identify a potential transcriptional regulatory network underlying this susceptible response, which contribute to a better understanding of the potato–S. endobioticum pathosystem.
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spelling pubmed-92773942022-07-14 Integrative Analysis of Genes Involved in the Global Response to Potato Wart Formation Yan, Lang Li, Yan Qing, Yuan Tao, Xiang Wang, Haiyan Lai, Xianjun Zhang, Yizheng Front Plant Sci Plant Science Synchytrium endobioticum, the causal agent of potato wart disease, poses a major threat to commercial potato production. Understanding the roles of transcriptionally regulated genes following pathogen infection is necessary for understanding the system-level host response to pathogen. Although some understanding of defense mechanisms against S. endobioticum infection has been gained for incompatible interactions, the genes and signaling pathways involved in the compatible interaction remain unclear. Based on the collection of wart diseased tubers of a susceptible cultivar, we performed phenotypic and dual RNA-Seq analyses of wart lesions in seven stages of disease progression. We totally detected 5,052 differentially expressed genes (DEGs) by comparing the different stages of infection to uninfected controls. The tendency toward differential gene expression was active rather than suppressed under attack by the pathogen. The number of DEGs step-up along with the development of the disease and the first, third and seventh of the disease stages showed substantially increase of DEGs in comparison of the previous stage. The important functional groups identified via Gene ontology (GO) and KEGG enrichment were those responsible for plant-pathogen interaction, fatty acid elongation and phenylpropanoid biosynthesis. Gene coexpression networks, composed of 17 distinct gene modules that contained between 25 and 813 genes, revealed high interconnectivity of the induced response and led to the identification of a number of hub genes enriched at different stages of infection. These results provide a comprehensive perspective on the global response of potato to S. endobioticum infection and identify a potential transcriptional regulatory network underlying this susceptible response, which contribute to a better understanding of the potato–S. endobioticum pathosystem. Frontiers Media S.A. 2022-06-29 /pmc/articles/PMC9277394/ /pubmed/35845669 http://dx.doi.org/10.3389/fpls.2022.865716 Text en Copyright © 2022 Yan, Li, Qing, Tao, Wang, Lai and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yan, Lang
Li, Yan
Qing, Yuan
Tao, Xiang
Wang, Haiyan
Lai, Xianjun
Zhang, Yizheng
Integrative Analysis of Genes Involved in the Global Response to Potato Wart Formation
title Integrative Analysis of Genes Involved in the Global Response to Potato Wart Formation
title_full Integrative Analysis of Genes Involved in the Global Response to Potato Wart Formation
title_fullStr Integrative Analysis of Genes Involved in the Global Response to Potato Wart Formation
title_full_unstemmed Integrative Analysis of Genes Involved in the Global Response to Potato Wart Formation
title_short Integrative Analysis of Genes Involved in the Global Response to Potato Wart Formation
title_sort integrative analysis of genes involved in the global response to potato wart formation
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277394/
https://www.ncbi.nlm.nih.gov/pubmed/35845669
http://dx.doi.org/10.3389/fpls.2022.865716
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