Cargando…

Polar mutagenesis of polycistronic bacterial transcriptional units using Cas12a

BACKGROUND: Functionally related genes in bacteria are often organized and transcribed as polycistronic transcriptional units. Examples are the fim operon, which codes for biogenesis of type 1 fimbriae in Escherichia coli, and the atp operon, which codes for the FoF1 ATP synthase. We tested the hypo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Graffeuil, Antoine, Guerrero-Castro, Julio, Assefa, Aster, Uhlin, Bernt Eric, Cisneros, David A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277811/ https://www.ncbi.nlm.nih.gov/pubmed/35831865 http://dx.doi.org/10.1186/s12934-022-01844-y

Ejemplares similares

Efficient multiplex mutagenesis by RNA-guided Cas9 and its use in the characterization of regulatory elements in the AGAMOUS gene
por: Yan, Wenhao, et al.
Publicado: (2016)

Translation initiation of leaderless and polycistronic transcripts in mammalian mitochondria
por: Remes, Cristina, et al.
Publicado: (2023)

Extensive Polycistronism and Antisense Transcription in the Mammalian Hox Clusters
por: Mainguy, Gaëll, et al.
Publicado: (2007)

Regulation of gene expression in trypanosomatids: living with polycistronic transcription
por: Clayton, Christine
Publicado: (2019)

“Myc’ed Messages”: Myc Induces Transcription of E2F1 while Inhibiting Its Translation via a microRNA Polycistron
por: Coller, Hilary A, et al.
Publicado: (2007)

Histone acetylations mark origins of polycistronic transcription in Leishmania major
por: Thomas, Sean, et al.
Publicado: (2009)

CRISPR–Cas-mediated transcriptional control and epi-mutagenesis
por: Gardiner, Jason, et al.
Publicado: (2022)

Synthetic polycistronic sequences in eukaryotes
por: Wang, Xuekun, et al.
Publicado: (2021)

Drosophila balancer reengineering using polycistronic gRNA for CRISPR/Cas9 gene editing
por: Benner, Leif, et al.
Publicado: (2018)

SEVA-Cpf1, a CRISPR-Cas12a vector for genome editing in cyanobacteria
por: Baldanta, Sara, et al.
Publicado: (2022)

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
por: Lee, Keunsub, et al.
Publicado: (2018)

Deletion mutagenesis of large areas in Plasmodium falciparum genes: a comparative study
por: Williams, Marni, et al.
Publicado: (2007)

Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris
por: Tachioka, Mikako, et al.
Publicado: (2016)

Widespread Polycistronic Transcripts in Fungi Revealed by Single-Molecule mRNA Sequencing
por: Gordon, Sean P., et al.
Publicado: (2015)

Third-generation Sequencing Reveals Extensive Polycistronism and Transcriptional Overlapping in a Baculovirus
por: Moldován, Norbert, et al.
Publicado: (2018)

Polycistronic gene expression in Aspergillus niger
por: Schuetze, Tabea, et al.
Publicado: (2017)

Polycistronic Animal Virus mRNAs
por: Samuel, Charles E.
Publicado: (1989)

New approach for fish breeding by chemical mutagenesis: establishment of TILLING method in fugu (Takifugu rubripes) with ENU mutagenesis
por: Kuroyanagi, Miwa, et al.
Publicado: (2013)

Bacterial Nanotubes for Intimate Sharing
por: Dongre, Mitesh, et al.
Publicado: (2011)

Incorporation of a lambda phage recombination system and EGFP detection to simplify mutagenesis of Herpes simplex virus bacterial artificial chromosomes
por: Schmeisser, Falko, et al.
Publicado: (2007)

Targeted mutagenesis in rabbit using an engineered BhCas12b variant
por: Jia, Yingqi, et al.
Publicado: (2022)

CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in Shigella flexneri by Cytosine Base Editing
por: Sharma, Atin, et al.
Publicado: (2022)

Widespread polycistronic gene expression in green algae
por: Gallaher, Sean D., et al.
Publicado: (2021)

Scarless and site-directed mutagenesis in Salmonella enteritidis chromosome
por: Cox, Mandy M, et al.
Publicado: (2007)

Reverse genetics through random mutagenesis in Histoplasma capsulatum
por: Youseff, Brian H, et al.
Publicado: (2009)

Efficient Targeted Mutagenesis Mediated by CRISPR-Cas12a Ribonucleoprotein Complexes in Maize
por: Dong, Shujie, et al.
Publicado: (2021)

EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations
por: Serrat, Xavier, et al.
Publicado: (2014)

Ribonuclease P processes polycistronic tRNA transcripts in Escherichia coli independent of ribonuclease E
por: Mohanty, Bijoy K., et al.
Publicado: (2007)

Nonsense-Mediated mRNA Decay Immunity Can Help Identify Human Polycistronic Transcripts
por: Shahaf, Guy, et al.
Publicado: (2014)

A polycistronic transgene design for combinatorial genetic perturbations from a single transcript in Drosophila
por: Teague, Alexander G., et al.
Publicado: (2023)

PredicTF: prediction of bacterial transcription factors in complex microbial communities using deep learning
por: Oliveira Monteiro, Lummy Maria, et al.
Publicado: (2022)

Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis thaliana
por: Kazama, Yusuke, et al.
Publicado: (2011)

Patch cloning method for multiple site-directed and saturation mutagenesis
por: Taniguchi, Naohiro, et al.
Publicado: (2013)

Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity
por: Huang, Hongxin, et al.
Publicado: (2022)

RANGE: Gene Transfer of Reversibly Controlled Polycistronic Genes
por: Chen, Yiwei, et al.
Publicado: (2013)

ADAR mediates differential expression of polycistronic microRNAs
por: Chawla, Geetanjali, et al.
Publicado: (2014)

Novel regulation and functional interaction of polycistronic miRNAs
por: Truscott, Mary, et al.
Publicado: (2016)

Polycistronic trypanosome mRNAs are a target for the exosome
por: Kramer, Susanne, et al.
Publicado: (2016)

Establishing Polycistronic Expression in the Model Microorganism Ustilago maydis
por: Müntjes, Kira, et al.
Publicado: (2020)

Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid
por: Naorem, Santa S., et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_hgb0dpobcotn7gv6v8i50qpth4