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Polar mutagenesis of polycistronic bacterial transcriptional units using Cas12a

BACKGROUND: Functionally related genes in bacteria are often organized and transcribed as polycistronic transcriptional units. Examples are the fim operon, which codes for biogenesis of type 1 fimbriae in Escherichia coli, and the atp operon, which codes for the FoF1 ATP synthase. We tested the hypo...

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Detalles Bibliográficos
Autores principales:	Graffeuil, Antoine, Guerrero-Castro, Julio, Assefa, Aster, Uhlin, Bernt Eric, Cisneros, David A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277811/ https://www.ncbi.nlm.nih.gov/pubmed/35831865 http://dx.doi.org/10.1186/s12934-022-01844-y

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