Cargando…
The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs
BACKGROUND: Antimicrobial peptides (AMPs) participate in the humoral immune response of insects eliminating invasive microorganisms. The immune deficiency pathway (IMD) and Toll are the main pathways by which the synthesis of these molecules is regulated in response to Gram-negative (IMD pathway) or...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277830/ https://www.ncbi.nlm.nih.gov/pubmed/35821152 http://dx.doi.org/10.1186/s13071-022-05363-y |
_version_ | 1784746065604378624 |
---|---|
author | Alejandro, Alvarado-Delgado Lilia, Juárez-Palma Jesús, Maritinez-Bartneche Henry, Rodriguez Mario |
author_facet | Alejandro, Alvarado-Delgado Lilia, Juárez-Palma Jesús, Maritinez-Bartneche Henry, Rodriguez Mario |
author_sort | Alejandro, Alvarado-Delgado |
collection | PubMed |
description | BACKGROUND: Antimicrobial peptides (AMPs) participate in the humoral immune response of insects eliminating invasive microorganisms. The immune deficiency pathway (IMD) and Toll are the main pathways by which the synthesis of these molecules is regulated in response to Gram-negative (IMD pathway) or Gram-positive (Toll pathway) bacteria. Various pattern-recognition receptors (PRRs) participate in the recognition of microorganisms, such as pgrp-lc and toll, which trigger signaling cascades and activate NF-κB family transcription factors, such as relish, that translocate to the cell nucleus, mainly in the fat body, inducing AMP gene transcription. METHODS: T. pallidipennis inhibited in Tppgrp-lc, Tptoll, and Tprelish were challenged with E. coli and M. luteus to analyze the expression of AMPs transcripts in the fat body and to execute survival assays. RESULTS: In this work we investigated the participation of the pgrp-lc and toll receptor genes and the relish transcription factor (designated as Tppgrp-lc, Tptoll, and Tprelish), in the transcriptional regulation of defensin B, prolixicin, and lysozyme B in Triatoma pallidipennis, one of the main vectors of Chagas disease. AMP transcript abundance was higher in the fat body of blood-fed than non-fed bugs. Challenge with Escherichia coli or Micrococcus luteus induced differential increases in AMP transcripts. Additionally, silencing of Tppgrp-lc, Tptoll, and Tprelish resulted in reduced AMP transcription and survival of bugs after a bacterial challenge. CONCLUSIONS: Our findings demonstrated that the IMD and Toll pathways in T. pallidipennis preferentially respond to Gram-negative and Gram-positive bacteria, respectively, by increasing the expression of AMP transcripts, but cross-induction also occurs. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-022-05363-y. |
format | Online Article Text |
id | pubmed-9277830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92778302022-07-14 The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs Alejandro, Alvarado-Delgado Lilia, Juárez-Palma Jesús, Maritinez-Bartneche Henry, Rodriguez Mario Parasit Vectors Research BACKGROUND: Antimicrobial peptides (AMPs) participate in the humoral immune response of insects eliminating invasive microorganisms. The immune deficiency pathway (IMD) and Toll are the main pathways by which the synthesis of these molecules is regulated in response to Gram-negative (IMD pathway) or Gram-positive (Toll pathway) bacteria. Various pattern-recognition receptors (PRRs) participate in the recognition of microorganisms, such as pgrp-lc and toll, which trigger signaling cascades and activate NF-κB family transcription factors, such as relish, that translocate to the cell nucleus, mainly in the fat body, inducing AMP gene transcription. METHODS: T. pallidipennis inhibited in Tppgrp-lc, Tptoll, and Tprelish were challenged with E. coli and M. luteus to analyze the expression of AMPs transcripts in the fat body and to execute survival assays. RESULTS: In this work we investigated the participation of the pgrp-lc and toll receptor genes and the relish transcription factor (designated as Tppgrp-lc, Tptoll, and Tprelish), in the transcriptional regulation of defensin B, prolixicin, and lysozyme B in Triatoma pallidipennis, one of the main vectors of Chagas disease. AMP transcript abundance was higher in the fat body of blood-fed than non-fed bugs. Challenge with Escherichia coli or Micrococcus luteus induced differential increases in AMP transcripts. Additionally, silencing of Tppgrp-lc, Tptoll, and Tprelish resulted in reduced AMP transcription and survival of bugs after a bacterial challenge. CONCLUSIONS: Our findings demonstrated that the IMD and Toll pathways in T. pallidipennis preferentially respond to Gram-negative and Gram-positive bacteria, respectively, by increasing the expression of AMP transcripts, but cross-induction also occurs. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-022-05363-y. BioMed Central 2022-07-12 /pmc/articles/PMC9277830/ /pubmed/35821152 http://dx.doi.org/10.1186/s13071-022-05363-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Alejandro, Alvarado-Delgado Lilia, Juárez-Palma Jesús, Maritinez-Bartneche Henry, Rodriguez Mario The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs |
title | The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs |
title_full | The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs |
title_fullStr | The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs |
title_full_unstemmed | The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs |
title_short | The IMD and Toll canonical immune pathways of Triatoma pallidipennis are preferentially activated by Gram-negative and Gram-positive bacteria, respectively, but cross-activation also occurs |
title_sort | imd and toll canonical immune pathways of triatoma pallidipennis are preferentially activated by gram-negative and gram-positive bacteria, respectively, but cross-activation also occurs |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277830/ https://www.ncbi.nlm.nih.gov/pubmed/35821152 http://dx.doi.org/10.1186/s13071-022-05363-y |
work_keys_str_mv | AT alejandroalvaradodelgado theimdandtollcanonicalimmunepathwaysoftriatomapallidipennisarepreferentiallyactivatedbygramnegativeandgrampositivebacteriarespectivelybutcrossactivationalsooccurs AT liliajuarezpalma theimdandtollcanonicalimmunepathwaysoftriatomapallidipennisarepreferentiallyactivatedbygramnegativeandgrampositivebacteriarespectivelybutcrossactivationalsooccurs AT jesusmaritinezbartneche theimdandtollcanonicalimmunepathwaysoftriatomapallidipennisarepreferentiallyactivatedbygramnegativeandgrampositivebacteriarespectivelybutcrossactivationalsooccurs AT henryrodriguezmario theimdandtollcanonicalimmunepathwaysoftriatomapallidipennisarepreferentiallyactivatedbygramnegativeandgrampositivebacteriarespectivelybutcrossactivationalsooccurs AT alejandroalvaradodelgado imdandtollcanonicalimmunepathwaysoftriatomapallidipennisarepreferentiallyactivatedbygramnegativeandgrampositivebacteriarespectivelybutcrossactivationalsooccurs AT liliajuarezpalma imdandtollcanonicalimmunepathwaysoftriatomapallidipennisarepreferentiallyactivatedbygramnegativeandgrampositivebacteriarespectivelybutcrossactivationalsooccurs AT jesusmaritinezbartneche imdandtollcanonicalimmunepathwaysoftriatomapallidipennisarepreferentiallyactivatedbygramnegativeandgrampositivebacteriarespectivelybutcrossactivationalsooccurs AT henryrodriguezmario imdandtollcanonicalimmunepathwaysoftriatomapallidipennisarepreferentiallyactivatedbygramnegativeandgrampositivebacteriarespectivelybutcrossactivationalsooccurs |