Cargando…

DNA extraction of bacterial cells using a semi-automated filtration system

The COVID-19 pandemic lockdown created problems with importing of commercial kits resulting in extended turnaround times for consumable deliveries. One way to circumvent this was to use an inexpensive optimized in-house method for DNA extraction from water. • The DNA extraction methods were optimize...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hoorzook, K.B., Barnard, T.G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Method Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277997/ https://www.ncbi.nlm.nih.gov/pubmed/35855950 http://dx.doi.org/10.1016/j.mex.2022.101785

Ejemplares similares

Culture independent DNA extraction method for bacterial cells concentrated from water
por: Hoorzook, K.B., et al.
Publicado: (2022)

Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples
por: Hoorzook, K. B., et al.
Publicado: (2021)

Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones
por: Frank, Elie, et al.
Publicado: (2023)

Method for semi-automated microscopy of filtration-enriched circulating tumor cells
por: Pailler, Emma, et al.
Publicado: (2016)

Semi-automated segmentation of the lateral periventricular regions using diffusion magnetic resonance imaging
por: Isaacs, Albert M., et al.
Publicado: (2020)

An efficient method for high molecular weight bacterial DNA extraction suitable for shotgun metagenomics from skin swabs
por: Serghiou, Iliana R., et al.
Publicado: (2023)

HOPS: automated detection and authentication of pathogen DNA in archaeological remains
por: Hübler, Ron, et al.
Publicado: (2019)

Emulsified BMVC derivative induced filtration for G-quadruplex DNA structural separation
por: Tsai, Yu-Lin, et al.
Publicado: (2011)

Gravity filtration of environmental DNA: A simple, fast, and power-free method
por: Oka, Shin-ichiro, et al.
Publicado: (2022)

SLiCE: a novel bacterial cell extract-based DNA cloning method
por: Zhang, Yongwei, et al.
Publicado: (2012)

Coexistence of free-living amoebae and bacteria in selected South African hospital water distribution systems
por: Muchesa, P., et al.
Publicado: (2016)

A customized framework for regional classification of conifers using automated feature extraction
por: Roth, Cali L., et al.
Publicado: (2021)

A semi-automated high-throughput approach to the generation of transposon insertion mutants in the nematode Caenorhabditis elegans
por: Duverger, Yohann, et al.
Publicado: (2007)

Automated concept and relationship extraction for the semi-automated ontology management (SEAM) system
por: Doing-Harris, Kristina, et al.
Publicado: (2015)

OASIS: an automated program for global investigation of bacterial and archaeal insertion sequences
por: Robinson, David G., et al.
Publicado: (2012)

A semi-automated protocol for Archaea DNA extraction from stools
por: Khelaifia, Saber, et al.
Publicado: (2013)

Soul of the Jukskei River: The Extent of Bacterial Contamination in the Jukskei River in Gauteng Province, South Africa
por: Hoorzook, Kousar Banu, et al.
Publicado: (2021)

Data extraction for epidemiological research (DExtER): a novel tool for automated clinical epidemiology studies
por: Gokhale, Krishna Margadhamane, et al.
Publicado: (2020)

Multiplex transcriptional characterizations across diverse bacterial species using cell‐free systems
por: Yim, Sung Sun, et al.
Publicado: (2019)

Validation of semi-automated flow-mediated dilation measurement in healthy volunteers
por: Dobbie, Laurence J., et al.
Publicado: (2020)

Evaluation of an Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics
por: Meslier, Victoria, et al.
Publicado: (2023)

Detection of ESKAPE Bacterial Pathogens at the Point of Care Using Isothermal DNA-Based Assays in a Portable Degas-Actuated Microfluidic Diagnostic Assay Platform
por: Renner, Lars D., et al.
Publicado: (2017)

Automated quality control and cell identification of droplet-based single-cell data using dropkick
por: Heiser, Cody N., et al.
Publicado: (2021)

RNA cytometry of single-cells using semi-permeable microcapsules
por: Leonaviciene, Greta, et al.
Publicado: (2022)

MyelTracer: A Semi-Automated Software for Myelin g-Ratio Quantification
por: Kaiser, Tobias, et al.
Publicado: (2021)

Accurate quantification of bacterial abundance in metagenomic DNAs accounting for variable DNA integrity levels
por: Manzari, Caterina, et al.
Publicado: (2020)

Circlator: automated circularization of genome assemblies using long sequencing reads
por: Hunt, Martin, et al.
Publicado: (2015)

A minimally destructive protocol for DNA extraction from ancient teeth
por: Harney, Éadaoin, et al.
Publicado: (2021)

Fast Adipogenesis Tracking System (FATS)—a robust, high-throughput, automation-ready adipogenesis quantification technique
por: Yuan, Chengxiang, et al.
Publicado: (2019)

Autometa: automated extraction of microbial genomes from individual shotgun metagenomes
por: Miller, Ian J, et al.
Publicado: (2019)

YTLR: Extracting yeast transcription factor-gene associations from the literature using automated literature readers
por: Yang, Tzu-Hsien, et al.
Publicado: (2022)

DISC: a highly scalable and accurate inference of gene expression and structure for single-cell transcriptomes using semi-supervised deep learning
por: He, Yao, et al.
Publicado: (2020)

A Semi-Automated Workflow for Brain Slice Histology Alignment, Registration, and Cell Quantification (SHARCQ)
por: Lauridsen, Kristoffer, et al.
Publicado: (2022)

An Improved Methodology to Overcome Key Issues in Human Fecal Metagenomic DNA Extraction
por: Kumar, Jitendra, et al.
Publicado: (2016)

Evaluation of DNA damage using single-cell gel electrophoresis (Comet Assay)
por: Nandhakumar, S., et al.
Publicado: (2011)

Automated annotation of human centromeres with HORmon
por: Kunyavskaya, Olga, et al.
Publicado: (2022)

Visualizing the Translocation and Localization of Bacterial Type III Effector Proteins by Using a Genetically Encoded Reporter System
por: Gawthorne, Jayde A., et al.
Publicado: (2016)

A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis
por: Song, Fangchao, et al.
Publicado: (2021)

A universal method for automated gene mapping
por: Zipperlen, Peder, et al.
Publicado: (2005)

Automated staging of zebrafish embryos with deep learning
por: Jones, Rebecca A, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_52tqrdmrt3l83dicgmgqp6dj4c