Cargando…
New Enclosure for in vivo Medical Imaging of Zebrafish With Vital Signs Monitoring
Lately, the use of zebrafish has gained increased interest in the scientific community as an animal model in preclinical research. However, there is a lack of in vivo imaging tools that ensure animal welfare during acquisition procedures. The use of functional imaging techniques, like Positron Emiss...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278279/ https://www.ncbi.nlm.nih.gov/pubmed/35846002 http://dx.doi.org/10.3389/fphys.2022.906110 |
_version_ | 1784746153617653760 |
---|---|
author | Magalhães, A. C. M. Correia, P. M. M. Oliveira, R. G. Encarnação, P. M. C. C. Domingues, I. Veloso, J. F. C. A. Silva, A. L. M. |
author_facet | Magalhães, A. C. M. Correia, P. M. M. Oliveira, R. G. Encarnação, P. M. C. C. Domingues, I. Veloso, J. F. C. A. Silva, A. L. M. |
author_sort | Magalhães, A. C. M. |
collection | PubMed |
description | Lately, the use of zebrafish has gained increased interest in the scientific community as an animal model in preclinical research. However, there is a lack of in vivo imaging tools that ensure animal welfare during acquisition procedures. The use of functional imaging techniques, like Positron Emission Tomography (PET), in zebrafish is limited since it requires the animal to be alive, representing a higher instrumentation complexity when compared to morphological imaging systems. In the present work, a new zebrafish enclosure was developed to acquire in vivo images while monitoring the animal’s welfare through its heartbeat. The temperature, dissolved oxygen, and pH range in a closed aquatic environment were tested to ensure that the conditions stay suitable for animal welfare during image acquisitions. The developed system, based on an enclosure with a bed and heartbeat sensors, was tested under controlled conditions in anesthetized fishes. Since the anesthetized zebrafish do not affect the water quality over time, there is no need to incorporate water circulation for the expected time of PET exams (about 30 min). The range of values obtained for the zebrafish heart rate was 88–127 bpm. The developed system has shown promising results regarding the zebrafish’s heart rate while keeping the fish still during the long imaging exams. The zebrafish enclosure ensures the animal’s well-being during the acquisition of in vivo images in different modalities (PET, Computer Tomography, Magnetic Resonance Imaging), contributing substantially to the preclinical research. |
format | Online Article Text |
id | pubmed-9278279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92782792022-07-14 New Enclosure for in vivo Medical Imaging of Zebrafish With Vital Signs Monitoring Magalhães, A. C. M. Correia, P. M. M. Oliveira, R. G. Encarnação, P. M. C. C. Domingues, I. Veloso, J. F. C. A. Silva, A. L. M. Front Physiol Physiology Lately, the use of zebrafish has gained increased interest in the scientific community as an animal model in preclinical research. However, there is a lack of in vivo imaging tools that ensure animal welfare during acquisition procedures. The use of functional imaging techniques, like Positron Emission Tomography (PET), in zebrafish is limited since it requires the animal to be alive, representing a higher instrumentation complexity when compared to morphological imaging systems. In the present work, a new zebrafish enclosure was developed to acquire in vivo images while monitoring the animal’s welfare through its heartbeat. The temperature, dissolved oxygen, and pH range in a closed aquatic environment were tested to ensure that the conditions stay suitable for animal welfare during image acquisitions. The developed system, based on an enclosure with a bed and heartbeat sensors, was tested under controlled conditions in anesthetized fishes. Since the anesthetized zebrafish do not affect the water quality over time, there is no need to incorporate water circulation for the expected time of PET exams (about 30 min). The range of values obtained for the zebrafish heart rate was 88–127 bpm. The developed system has shown promising results regarding the zebrafish’s heart rate while keeping the fish still during the long imaging exams. The zebrafish enclosure ensures the animal’s well-being during the acquisition of in vivo images in different modalities (PET, Computer Tomography, Magnetic Resonance Imaging), contributing substantially to the preclinical research. Frontiers Media S.A. 2022-06-29 /pmc/articles/PMC9278279/ /pubmed/35846002 http://dx.doi.org/10.3389/fphys.2022.906110 Text en Copyright © 2022 Magalhães, Correia, Oliveira, Encarnação, Domingues, Veloso and Silva. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Magalhães, A. C. M. Correia, P. M. M. Oliveira, R. G. Encarnação, P. M. C. C. Domingues, I. Veloso, J. F. C. A. Silva, A. L. M. New Enclosure for in vivo Medical Imaging of Zebrafish With Vital Signs Monitoring |
title | New Enclosure for in vivo Medical Imaging of Zebrafish With Vital Signs Monitoring |
title_full | New Enclosure for in vivo Medical Imaging of Zebrafish With Vital Signs Monitoring |
title_fullStr | New Enclosure for in vivo Medical Imaging of Zebrafish With Vital Signs Monitoring |
title_full_unstemmed | New Enclosure for in vivo Medical Imaging of Zebrafish With Vital Signs Monitoring |
title_short | New Enclosure for in vivo Medical Imaging of Zebrafish With Vital Signs Monitoring |
title_sort | new enclosure for in vivo medical imaging of zebrafish with vital signs monitoring |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278279/ https://www.ncbi.nlm.nih.gov/pubmed/35846002 http://dx.doi.org/10.3389/fphys.2022.906110 |
work_keys_str_mv | AT magalhaesacm newenclosureforinvivomedicalimagingofzebrafishwithvitalsignsmonitoring AT correiapmm newenclosureforinvivomedicalimagingofzebrafishwithvitalsignsmonitoring AT oliveirarg newenclosureforinvivomedicalimagingofzebrafishwithvitalsignsmonitoring AT encarnacaopmcc newenclosureforinvivomedicalimagingofzebrafishwithvitalsignsmonitoring AT dominguesi newenclosureforinvivomedicalimagingofzebrafishwithvitalsignsmonitoring AT velosojfca newenclosureforinvivomedicalimagingofzebrafishwithvitalsignsmonitoring AT silvaalm newenclosureforinvivomedicalimagingofzebrafishwithvitalsignsmonitoring |