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A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim. We report the engineering of a bright red flu...

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Detalles Bibliográficos
Autores principales: Ning, Lin, Geng, Yang, Lovett-Barron, Matthew, Niu, Xiaoman, Deng, Mengying, Wang, Liang, Ataie, Niloufar, Sens, Alex, Ng, Ho-Leung, Chen, Shoudeng, Deisseroth, Karl, Lin, Michael Z., Chu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278655/
https://www.ncbi.nlm.nih.gov/pubmed/35846353
http://dx.doi.org/10.3389/fcell.2022.893468
Descripción
Sumario:Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim. We report the engineering of a bright red fluorescent protein, Crimson, that enables long-term morphological labeling of neurons without aggregation or toxicity. Crimson is similar to mCherry and mKate2 in fluorescence spectra but is 100 and 28% greater in molecular brightness, respectively. We used a membrane-localized Crimson-CAAX to label thin neurites, dendritic spines and filopodia, enhancing detection of these small structures compared to cytosolic markers.