Cargando…
LncRNA MBNL1-AS1 represses gastric cancer progression via the TGF-β pathway by modulating miR-424-5p/Smad7 axis
Studies over the past decades have implicated lncRNAs in promoting the development, migration and invasion of gastric cancer (GC). However, the role and mechanism of lncRNA MBNL1-AS1 in GC promotion are poorly understood. In this research, qRT-PCR showed that MBNL1-AS1 was down-regulated in GC tissu...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278977/ https://www.ncbi.nlm.nih.gov/pubmed/35311623 http://dx.doi.org/10.1080/21655979.2022.2037921 |
_version_ | 1784746292302315520 |
---|---|
author | Su, Jiewen Chen, Dawei Ruan, Yi Tian, Yuan Lv, Kaiji Zhou, Xinhua Ying, Dongjian Lu, Yeting |
author_facet | Su, Jiewen Chen, Dawei Ruan, Yi Tian, Yuan Lv, Kaiji Zhou, Xinhua Ying, Dongjian Lu, Yeting |
author_sort | Su, Jiewen |
collection | PubMed |
description | Studies over the past decades have implicated lncRNAs in promoting the development, migration and invasion of gastric cancer (GC). However, the role and mechanism of lncRNA MBNL1-AS1 in GC promotion are poorly understood. In this research, qRT-PCR showed that MBNL1-AS1 was down-regulated in GC tissues and cells. Cell experiments and the animal study demonstrated that MBNL1-AS1 knockdown accelerated GC cell proliferation, migration, and invasion, thus restraining cell apoptosis. Meanwhile, overexpression of MBNL1-AS1 repressed GC cell promotion. Bioinformatics analysis confirmed that MBNL1-AS1 binds to miR-424-5p via negative modulation. Rescue experiments showed that decreased miR-424-5p level inhibited GC cell promotion by silencing MBNL1-AS1. Furthermore, Smad7 was identified as a target of miR-424-5p that could reverse the promotion of GC cell growth mediated by miR-424-5p. Western blot results proved that MBNL1-AS1 affected TGF-β/SMAD pathways by regulating the miR-424-5p/Smad7 axis. Collectively, MBNL1-AS1 restrained GC growth via the miR-424-5p/Smad7 axis and thus could be a promising target for GC therapy. These findings illustrate that lncRNA MBNL1-AS1, as a tumor suppressor gene, participates in GC progression by regulating miR-424-5p/Smad7 axis, thus activating TGF-β/EMT pathways. The evidence may provide a potential marker for GC patients. |
format | Online Article Text |
id | pubmed-9278977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-92789772022-07-14 LncRNA MBNL1-AS1 represses gastric cancer progression via the TGF-β pathway by modulating miR-424-5p/Smad7 axis Su, Jiewen Chen, Dawei Ruan, Yi Tian, Yuan Lv, Kaiji Zhou, Xinhua Ying, Dongjian Lu, Yeting Bioengineered Research Paper Studies over the past decades have implicated lncRNAs in promoting the development, migration and invasion of gastric cancer (GC). However, the role and mechanism of lncRNA MBNL1-AS1 in GC promotion are poorly understood. In this research, qRT-PCR showed that MBNL1-AS1 was down-regulated in GC tissues and cells. Cell experiments and the animal study demonstrated that MBNL1-AS1 knockdown accelerated GC cell proliferation, migration, and invasion, thus restraining cell apoptosis. Meanwhile, overexpression of MBNL1-AS1 repressed GC cell promotion. Bioinformatics analysis confirmed that MBNL1-AS1 binds to miR-424-5p via negative modulation. Rescue experiments showed that decreased miR-424-5p level inhibited GC cell promotion by silencing MBNL1-AS1. Furthermore, Smad7 was identified as a target of miR-424-5p that could reverse the promotion of GC cell growth mediated by miR-424-5p. Western blot results proved that MBNL1-AS1 affected TGF-β/SMAD pathways by regulating the miR-424-5p/Smad7 axis. Collectively, MBNL1-AS1 restrained GC growth via the miR-424-5p/Smad7 axis and thus could be a promising target for GC therapy. These findings illustrate that lncRNA MBNL1-AS1, as a tumor suppressor gene, participates in GC progression by regulating miR-424-5p/Smad7 axis, thus activating TGF-β/EMT pathways. The evidence may provide a potential marker for GC patients. Taylor & Francis 2022-03-21 /pmc/articles/PMC9278977/ /pubmed/35311623 http://dx.doi.org/10.1080/21655979.2022.2037921 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Su, Jiewen Chen, Dawei Ruan, Yi Tian, Yuan Lv, Kaiji Zhou, Xinhua Ying, Dongjian Lu, Yeting LncRNA MBNL1-AS1 represses gastric cancer progression via the TGF-β pathway by modulating miR-424-5p/Smad7 axis |
title | LncRNA MBNL1-AS1 represses gastric cancer progression via the TGF-β pathway by modulating miR-424-5p/Smad7 axis |
title_full | LncRNA MBNL1-AS1 represses gastric cancer progression via the TGF-β pathway by modulating miR-424-5p/Smad7 axis |
title_fullStr | LncRNA MBNL1-AS1 represses gastric cancer progression via the TGF-β pathway by modulating miR-424-5p/Smad7 axis |
title_full_unstemmed | LncRNA MBNL1-AS1 represses gastric cancer progression via the TGF-β pathway by modulating miR-424-5p/Smad7 axis |
title_short | LncRNA MBNL1-AS1 represses gastric cancer progression via the TGF-β pathway by modulating miR-424-5p/Smad7 axis |
title_sort | lncrna mbnl1-as1 represses gastric cancer progression via the tgf-β pathway by modulating mir-424-5p/smad7 axis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278977/ https://www.ncbi.nlm.nih.gov/pubmed/35311623 http://dx.doi.org/10.1080/21655979.2022.2037921 |
work_keys_str_mv | AT sujiewen lncrnambnl1as1repressesgastriccancerprogressionviathetgfbpathwaybymodulatingmir4245psmad7axis AT chendawei lncrnambnl1as1repressesgastriccancerprogressionviathetgfbpathwaybymodulatingmir4245psmad7axis AT ruanyi lncrnambnl1as1repressesgastriccancerprogressionviathetgfbpathwaybymodulatingmir4245psmad7axis AT tianyuan lncrnambnl1as1repressesgastriccancerprogressionviathetgfbpathwaybymodulatingmir4245psmad7axis AT lvkaiji lncrnambnl1as1repressesgastriccancerprogressionviathetgfbpathwaybymodulatingmir4245psmad7axis AT zhouxinhua lncrnambnl1as1repressesgastriccancerprogressionviathetgfbpathwaybymodulatingmir4245psmad7axis AT yingdongjian lncrnambnl1as1repressesgastriccancerprogressionviathetgfbpathwaybymodulatingmir4245psmad7axis AT luyeting lncrnambnl1as1repressesgastriccancerprogressionviathetgfbpathwaybymodulatingmir4245psmad7axis |