Massively targeted evaluation of therapeutic CRISPR off-targets in cells

Methods for sensitive and high-throughput evaluation of CRISPR RNA-guided nucleases (RGNs) off-targets (OTs) are essential for advancing RGN-based gene therapies. Here we report SURRO-seq for simultaneously evaluating thousands of therapeutic RGN OTs in cells. SURRO-seq captures RGN-induced indels i...

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Detalles Bibliográficos
Autores principales: Pan, Xiaoguang, Qu, Kunli, Yuan, Hao, Xiang, Xi, Anthon, Christian, Pashkova, Liubov, Liang, Xue, Han, Peng, Corsi, Giulia I., Xu, Fengping, Liu, Ping, Zhong, Jiayan, Zhou, Yan, Ma, Tao, Jiang, Hui, Liu, Junnian, Wang, Jian, Jessen, Niels, Bolund, Lars, Yang, Huanming, Xu, Xun, Church, George M., Gorodkin, Jan, Lin, Lin, Luo, Yonglun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279339/
https://www.ncbi.nlm.nih.gov/pubmed/35831290
http://dx.doi.org/10.1038/s41467-022-31543-6

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