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Integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis

Sepsis has affected millions of populations of all age groups, locations, and sexes worldwide. Immune systems, either innate or adaptive are dysregulated due to the infection. Various biomarkers are present to date, still sepsis is a primary cause of mortality. Globally, post-operative body infectio...

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Autores principales: Mohsin, Mohd, Singh, Prithvi, Khan, Salman, Verma, Amit Kumar, Jha, Rishabh, Alsahli, Mohammed A., Rahmani, Arshad Husain, Almatroodi, Saleh A., Alrumaihi, Faris, Kaprwan, Nisha, Dev, Kapil, Dohare, Ravins, Syed, Mansoor Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279366/
https://www.ncbi.nlm.nih.gov/pubmed/35831411
http://dx.doi.org/10.1038/s41598-022-16183-6
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author Mohsin, Mohd
Singh, Prithvi
Khan, Salman
Verma, Amit Kumar
Jha, Rishabh
Alsahli, Mohammed A.
Rahmani, Arshad Husain
Almatroodi, Saleh A.
Alrumaihi, Faris
Kaprwan, Nisha
Dev, Kapil
Dohare, Ravins
Syed, Mansoor Ali
author_facet Mohsin, Mohd
Singh, Prithvi
Khan, Salman
Verma, Amit Kumar
Jha, Rishabh
Alsahli, Mohammed A.
Rahmani, Arshad Husain
Almatroodi, Saleh A.
Alrumaihi, Faris
Kaprwan, Nisha
Dev, Kapil
Dohare, Ravins
Syed, Mansoor Ali
author_sort Mohsin, Mohd
collection PubMed
description Sepsis has affected millions of populations of all age groups, locations, and sexes worldwide. Immune systems, either innate or adaptive are dysregulated due to the infection. Various biomarkers are present to date, still sepsis is a primary cause of mortality. Globally, post-operative body infections can cause sepsis and septic shock in ICU. Abnormal antigen presentation to T-cells leads to a dysregulated immune system. miRNAs are sparkly evolved as biomarkers due to their high sensitivity and efficiency. In this work, we analyzed high-throughput mRNA data collected from Gene Expression Omnibus (GEO) and linked it to significant miRNAs and TFs using a network-based approach. Protein–protein interaction (PPI) network was constructed using sepsis-specific differentially expressed genes (DEGs) followed by enrichment analyses and hub module detection. Sepsis-linked decrease transcription of the classical HLA gene such as HLA-DPB1 and its interplay with miR-let-7b-5p and transcription factor SPIB was observed. This study helped to provide innovative targets for sepsis.
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spelling pubmed-92793662022-07-15 Integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis Mohsin, Mohd Singh, Prithvi Khan, Salman Verma, Amit Kumar Jha, Rishabh Alsahli, Mohammed A. Rahmani, Arshad Husain Almatroodi, Saleh A. Alrumaihi, Faris Kaprwan, Nisha Dev, Kapil Dohare, Ravins Syed, Mansoor Ali Sci Rep Article Sepsis has affected millions of populations of all age groups, locations, and sexes worldwide. Immune systems, either innate or adaptive are dysregulated due to the infection. Various biomarkers are present to date, still sepsis is a primary cause of mortality. Globally, post-operative body infections can cause sepsis and septic shock in ICU. Abnormal antigen presentation to T-cells leads to a dysregulated immune system. miRNAs are sparkly evolved as biomarkers due to their high sensitivity and efficiency. In this work, we analyzed high-throughput mRNA data collected from Gene Expression Omnibus (GEO) and linked it to significant miRNAs and TFs using a network-based approach. Protein–protein interaction (PPI) network was constructed using sepsis-specific differentially expressed genes (DEGs) followed by enrichment analyses and hub module detection. Sepsis-linked decrease transcription of the classical HLA gene such as HLA-DPB1 and its interplay with miR-let-7b-5p and transcription factor SPIB was observed. This study helped to provide innovative targets for sepsis. Nature Publishing Group UK 2022-07-13 /pmc/articles/PMC9279366/ /pubmed/35831411 http://dx.doi.org/10.1038/s41598-022-16183-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mohsin, Mohd
Singh, Prithvi
Khan, Salman
Verma, Amit Kumar
Jha, Rishabh
Alsahli, Mohammed A.
Rahmani, Arshad Husain
Almatroodi, Saleh A.
Alrumaihi, Faris
Kaprwan, Nisha
Dev, Kapil
Dohare, Ravins
Syed, Mansoor Ali
Integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis
title Integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis
title_full Integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis
title_fullStr Integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis
title_full_unstemmed Integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis
title_short Integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, SPIB, and HLA-DPB1 in sepsis
title_sort integrated transcriptomic and regulatory network analyses uncovers the role of let-7b-5p, spib, and hla-dpb1 in sepsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279366/
https://www.ncbi.nlm.nih.gov/pubmed/35831411
http://dx.doi.org/10.1038/s41598-022-16183-6
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