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Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration

Early diagnosis of articular cartilage damage and repeated evaluation of treatment efficacy are essential for osteoarthritis treatment. In this study, we established a simple ultrasound grading system for early degenerative articular cartilage and investigated its relationship with cartilage biologi...

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Autores principales: Shi, Wen, Kanamoto, Takashi, Aihara, Masaharu, Oka, Shiro, Kuroda, Sanae, Nakai, Tsuyoshi, Mazuka, Takeo, Takenaka, Keisuke, Sato, Yuji, Tsukamoto, Masahiro, Ebina, Kosuke, Nakata, Ken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279397/
https://www.ncbi.nlm.nih.gov/pubmed/35831407
http://dx.doi.org/10.1038/s41598-022-16248-6
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author Shi, Wen
Kanamoto, Takashi
Aihara, Masaharu
Oka, Shiro
Kuroda, Sanae
Nakai, Tsuyoshi
Mazuka, Takeo
Takenaka, Keisuke
Sato, Yuji
Tsukamoto, Masahiro
Ebina, Kosuke
Nakata, Ken
author_facet Shi, Wen
Kanamoto, Takashi
Aihara, Masaharu
Oka, Shiro
Kuroda, Sanae
Nakai, Tsuyoshi
Mazuka, Takeo
Takenaka, Keisuke
Sato, Yuji
Tsukamoto, Masahiro
Ebina, Kosuke
Nakata, Ken
author_sort Shi, Wen
collection PubMed
description Early diagnosis of articular cartilage damage and repeated evaluation of treatment efficacy are essential for osteoarthritis treatment. In this study, we established a simple ultrasound grading system for early degenerative articular cartilage and investigated its relationship with cartilage biological characteristics. The ultrasound grading system were based on surface integrity (S1a: continuous high-echo lines, S1b: discontinuous or weak high-echo lines, S2: surface irregular) and cartilage echogenicity (E1: with > 50%, E2: < 50% hypoechoic area of total cartilage layer) and verified by surface roughness (Ra; μm) and histological staining. Ra was lower in S1 than in S2, and the percentage of hypoechoic and safranin O-stained areas was positively correlated. Then we examined its relationship with histopathological evaluation (OARSI grade), gene expression, and protein production in responded to pro-inflammatory cytokine (IL-1ß) stimulation. OARSI grades were different among S grades. The superficial layer of S1 had higher expression of Collagen10, aggrecan, Sox9, and lower expression of Collagen1 and BMP2 than that of S2. S1 responded more pronouncedly to IL-1ß in IL-6, IL-8, and CCL2 production than S2. There was no difference among the E-grades. Taken together, our findings indicate that ultrasound assessment using surface integrity can reflect the biological characteristics of early degenerative articular cartilage.
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spelling pubmed-92793972022-07-15 Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration Shi, Wen Kanamoto, Takashi Aihara, Masaharu Oka, Shiro Kuroda, Sanae Nakai, Tsuyoshi Mazuka, Takeo Takenaka, Keisuke Sato, Yuji Tsukamoto, Masahiro Ebina, Kosuke Nakata, Ken Sci Rep Article Early diagnosis of articular cartilage damage and repeated evaluation of treatment efficacy are essential for osteoarthritis treatment. In this study, we established a simple ultrasound grading system for early degenerative articular cartilage and investigated its relationship with cartilage biological characteristics. The ultrasound grading system were based on surface integrity (S1a: continuous high-echo lines, S1b: discontinuous or weak high-echo lines, S2: surface irregular) and cartilage echogenicity (E1: with > 50%, E2: < 50% hypoechoic area of total cartilage layer) and verified by surface roughness (Ra; μm) and histological staining. Ra was lower in S1 than in S2, and the percentage of hypoechoic and safranin O-stained areas was positively correlated. Then we examined its relationship with histopathological evaluation (OARSI grade), gene expression, and protein production in responded to pro-inflammatory cytokine (IL-1ß) stimulation. OARSI grades were different among S grades. The superficial layer of S1 had higher expression of Collagen10, aggrecan, Sox9, and lower expression of Collagen1 and BMP2 than that of S2. S1 responded more pronouncedly to IL-1ß in IL-6, IL-8, and CCL2 production than S2. There was no difference among the E-grades. Taken together, our findings indicate that ultrasound assessment using surface integrity can reflect the biological characteristics of early degenerative articular cartilage. Nature Publishing Group UK 2022-07-13 /pmc/articles/PMC9279397/ /pubmed/35831407 http://dx.doi.org/10.1038/s41598-022-16248-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shi, Wen
Kanamoto, Takashi
Aihara, Masaharu
Oka, Shiro
Kuroda, Sanae
Nakai, Tsuyoshi
Mazuka, Takeo
Takenaka, Keisuke
Sato, Yuji
Tsukamoto, Masahiro
Ebina, Kosuke
Nakata, Ken
Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration
title Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration
title_full Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration
title_fullStr Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration
title_full_unstemmed Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration
title_short Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration
title_sort articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279397/
https://www.ncbi.nlm.nih.gov/pubmed/35831407
http://dx.doi.org/10.1038/s41598-022-16248-6
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