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Comparison of gene expression profiles among caste differentiations in the termite Reticulitermes speratus

Termite castes express specialized phenotypes for their own tasks and are a good example of insect polyphenism. To understand the comprehensive gene expression profiles during caste differentiation, RNA-seq analysis based on the genome data was performed during the worker, presoldier, and nymphoid m...

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Autores principales: Saiki, Ryota, Hayashi, Yoshinobu, Toga, Kouhei, Yaguchi, Hajime, Masuoka, Yudai, Suzuki, Ryutaro, Fujiwara, Kokuto, Shigenobu, Shuji, Maekawa, Kiyoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279399/
https://www.ncbi.nlm.nih.gov/pubmed/35831400
http://dx.doi.org/10.1038/s41598-022-15984-z
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author Saiki, Ryota
Hayashi, Yoshinobu
Toga, Kouhei
Yaguchi, Hajime
Masuoka, Yudai
Suzuki, Ryutaro
Fujiwara, Kokuto
Shigenobu, Shuji
Maekawa, Kiyoto
author_facet Saiki, Ryota
Hayashi, Yoshinobu
Toga, Kouhei
Yaguchi, Hajime
Masuoka, Yudai
Suzuki, Ryutaro
Fujiwara, Kokuto
Shigenobu, Shuji
Maekawa, Kiyoto
author_sort Saiki, Ryota
collection PubMed
description Termite castes express specialized phenotypes for their own tasks and are a good example of insect polyphenism. To understand the comprehensive gene expression profiles during caste differentiation, RNA-seq analysis based on the genome data was performed during the worker, presoldier, and nymphoid molts in Reticulitermes speratus. In this species, artificial induction methods for each molt have already been established, and the time scale has been clarified. Three different periods (before the gut purge (GP), during the GP, and after the molt) were discriminated in each molt, and two body parts (head and other body regions) were separately sampled. The results revealed that many differentially expressed genes (head: 2884, body: 2579) were identified in each molt. Based on the independent real-time quantitative PCR analysis, we confirmed the different expression patterns of seven out of eight genes in the presoldier molt. Based on the GO and KEGG enrichment analyses, the expressions of genes related to juvenile hormone titer changes (e.g., JH acid methyltransferase), nutrition status (e.g., Acyl-CoA Delta desaturase), and cell proliferation (e.g., insulin receptor), were shown to specifically fluctuate in each molt. These differences may have a crucial impact on caste differentiation. These data are important resources for future termite sociogenomics.
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spelling pubmed-92793992022-07-15 Comparison of gene expression profiles among caste differentiations in the termite Reticulitermes speratus Saiki, Ryota Hayashi, Yoshinobu Toga, Kouhei Yaguchi, Hajime Masuoka, Yudai Suzuki, Ryutaro Fujiwara, Kokuto Shigenobu, Shuji Maekawa, Kiyoto Sci Rep Article Termite castes express specialized phenotypes for their own tasks and are a good example of insect polyphenism. To understand the comprehensive gene expression profiles during caste differentiation, RNA-seq analysis based on the genome data was performed during the worker, presoldier, and nymphoid molts in Reticulitermes speratus. In this species, artificial induction methods for each molt have already been established, and the time scale has been clarified. Three different periods (before the gut purge (GP), during the GP, and after the molt) were discriminated in each molt, and two body parts (head and other body regions) were separately sampled. The results revealed that many differentially expressed genes (head: 2884, body: 2579) were identified in each molt. Based on the independent real-time quantitative PCR analysis, we confirmed the different expression patterns of seven out of eight genes in the presoldier molt. Based on the GO and KEGG enrichment analyses, the expressions of genes related to juvenile hormone titer changes (e.g., JH acid methyltransferase), nutrition status (e.g., Acyl-CoA Delta desaturase), and cell proliferation (e.g., insulin receptor), were shown to specifically fluctuate in each molt. These differences may have a crucial impact on caste differentiation. These data are important resources for future termite sociogenomics. Nature Publishing Group UK 2022-07-13 /pmc/articles/PMC9279399/ /pubmed/35831400 http://dx.doi.org/10.1038/s41598-022-15984-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Saiki, Ryota
Hayashi, Yoshinobu
Toga, Kouhei
Yaguchi, Hajime
Masuoka, Yudai
Suzuki, Ryutaro
Fujiwara, Kokuto
Shigenobu, Shuji
Maekawa, Kiyoto
Comparison of gene expression profiles among caste differentiations in the termite Reticulitermes speratus
title Comparison of gene expression profiles among caste differentiations in the termite Reticulitermes speratus
title_full Comparison of gene expression profiles among caste differentiations in the termite Reticulitermes speratus
title_fullStr Comparison of gene expression profiles among caste differentiations in the termite Reticulitermes speratus
title_full_unstemmed Comparison of gene expression profiles among caste differentiations in the termite Reticulitermes speratus
title_short Comparison of gene expression profiles among caste differentiations in the termite Reticulitermes speratus
title_sort comparison of gene expression profiles among caste differentiations in the termite reticulitermes speratus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279399/
https://www.ncbi.nlm.nih.gov/pubmed/35831400
http://dx.doi.org/10.1038/s41598-022-15984-z
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