Cargando…

Molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae among children in China

Klebsiella pneumoniae infection and antimicrobial resistance among children are major concerns. The occurrence of hypervirulent K. pneumoniae (hvKp) infections is gradually increasing worldwide, and disinfectant resistance is also being reported. Carbapenem- and disinfectant-resistant hvKp infection...

Descripción completa

Detalles Bibliográficos
Autores principales: Jin, Zhengjiang, Wang, Zhenhui, Gong, Lin, Yi, Lu, Liu, Nian, Luo, Lan, Gong, Wenting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279541/
https://www.ncbi.nlm.nih.gov/pubmed/35829853
http://dx.doi.org/10.1186/s13568-022-01437-3
_version_ 1784746420365950976
author Jin, Zhengjiang
Wang, Zhenhui
Gong, Lin
Yi, Lu
Liu, Nian
Luo, Lan
Gong, Wenting
author_facet Jin, Zhengjiang
Wang, Zhenhui
Gong, Lin
Yi, Lu
Liu, Nian
Luo, Lan
Gong, Wenting
author_sort Jin, Zhengjiang
collection PubMed
description Klebsiella pneumoniae infection and antimicrobial resistance among children are major concerns. The occurrence of hypervirulent K. pneumoniae (hvKp) infections is gradually increasing worldwide, and disinfectant resistance is also being reported. Carbapenem- and disinfectant-resistant hvKp infection has made clinical treatment and nosocomial infection control among children increasingly challenging. In this study, whole-genome sequencing was conducted among 34 Carba NP-positive carbapenem-resistant K. pneumoniae (CRKP) strains, and the distribution of antibiotic resistance genes, virulence genes and disinfectant resistance genes was determined. Eleven distinct STs were identified, and most of them were ST11 (58.8%). Among the carbapenem resistance genes, KPC-2 was predominant (61.8%), followed by NDM-1 (26.5%) and IPM-4 (11.8%), and no other carbapenemase genes were found. Twelve virulence genes were investigated. All 34 CRKP strains carried the following virulence genes: rcsA/B, entA, fimA/H and mrkA/D. The gene iucB was present in only 3 (8.9%) CRKP strains. The positive detection rates of the iroN and ybtA genes were 94.1% and 64.7%, respectively. None of the strains was found to carry the rmpA and iroB genes. Two disinfectant resistance genes were investigated in this study. Twenty-one (61.8%) strains carried both the qacE and cepA disinfectant resistance genes, 13 (38.2%) CRKP strains carried only the cepA gene, and no strains with only the qacE gene was detected. The correlations among virulence, drug resistance and disinfectant tolerance showed that the virulence and disinfectant resistance genes were distinct among several types of carbapenemase-producing CRKP strains.
format Online
Article
Text
id pubmed-9279541
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-92795412022-07-15 Molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae among children in China Jin, Zhengjiang Wang, Zhenhui Gong, Lin Yi, Lu Liu, Nian Luo, Lan Gong, Wenting AMB Express Original Article Klebsiella pneumoniae infection and antimicrobial resistance among children are major concerns. The occurrence of hypervirulent K. pneumoniae (hvKp) infections is gradually increasing worldwide, and disinfectant resistance is also being reported. Carbapenem- and disinfectant-resistant hvKp infection has made clinical treatment and nosocomial infection control among children increasingly challenging. In this study, whole-genome sequencing was conducted among 34 Carba NP-positive carbapenem-resistant K. pneumoniae (CRKP) strains, and the distribution of antibiotic resistance genes, virulence genes and disinfectant resistance genes was determined. Eleven distinct STs were identified, and most of them were ST11 (58.8%). Among the carbapenem resistance genes, KPC-2 was predominant (61.8%), followed by NDM-1 (26.5%) and IPM-4 (11.8%), and no other carbapenemase genes were found. Twelve virulence genes were investigated. All 34 CRKP strains carried the following virulence genes: rcsA/B, entA, fimA/H and mrkA/D. The gene iucB was present in only 3 (8.9%) CRKP strains. The positive detection rates of the iroN and ybtA genes were 94.1% and 64.7%, respectively. None of the strains was found to carry the rmpA and iroB genes. Two disinfectant resistance genes were investigated in this study. Twenty-one (61.8%) strains carried both the qacE and cepA disinfectant resistance genes, 13 (38.2%) CRKP strains carried only the cepA gene, and no strains with only the qacE gene was detected. The correlations among virulence, drug resistance and disinfectant tolerance showed that the virulence and disinfectant resistance genes were distinct among several types of carbapenemase-producing CRKP strains. Springer Berlin Heidelberg 2022-07-12 /pmc/articles/PMC9279541/ /pubmed/35829853 http://dx.doi.org/10.1186/s13568-022-01437-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Jin, Zhengjiang
Wang, Zhenhui
Gong, Lin
Yi, Lu
Liu, Nian
Luo, Lan
Gong, Wenting
Molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae among children in China
title Molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae among children in China
title_full Molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae among children in China
title_fullStr Molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae among children in China
title_full_unstemmed Molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae among children in China
title_short Molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae among children in China
title_sort molecular epidemiological characteristics of carbapenem-resistant klebsiella pneumoniae among children in china
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279541/
https://www.ncbi.nlm.nih.gov/pubmed/35829853
http://dx.doi.org/10.1186/s13568-022-01437-3
work_keys_str_mv AT jinzhengjiang molecularepidemiologicalcharacteristicsofcarbapenemresistantklebsiellapneumoniaeamongchildreninchina
AT wangzhenhui molecularepidemiologicalcharacteristicsofcarbapenemresistantklebsiellapneumoniaeamongchildreninchina
AT gonglin molecularepidemiologicalcharacteristicsofcarbapenemresistantklebsiellapneumoniaeamongchildreninchina
AT yilu molecularepidemiologicalcharacteristicsofcarbapenemresistantklebsiellapneumoniaeamongchildreninchina
AT liunian molecularepidemiologicalcharacteristicsofcarbapenemresistantklebsiellapneumoniaeamongchildreninchina
AT luolan molecularepidemiologicalcharacteristicsofcarbapenemresistantklebsiellapneumoniaeamongchildreninchina
AT gongwenting molecularepidemiologicalcharacteristicsofcarbapenemresistantklebsiellapneumoniaeamongchildreninchina