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Deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder
OBJECTIVE: Recent studies have provided insights into the gut microbiota in autism spectrum disorder (ASD); however, these studies were restricted owing to limited sampling at the unitary stage of childhood. Herein, we aimed to reveal developmental characteristics of gut microbiota in a large cohort...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BMJ Publishing Group
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279844/ https://www.ncbi.nlm.nih.gov/pubmed/34930815 http://dx.doi.org/10.1136/gutjnl-2021-325115 |
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author | Lou, Mingxing Cao, Aihua Jin, Cuiyuan Mi, Kai Xiong, Xiyue Zeng, Zhaoyang Pan, Xu Qie, Jinlong Qiu, Shangfeng Niu, Yaofang Liang, Hao Liu, Yanping Chen, Lin Liu, Zhi Zhao, Qing Qiu, Xiyan Jin, Yuanxiang Sheng, Xiaoqi Hu, Zhibin Jin, Gulei Liu, Jingshi Liu, Xingyin Wang, Yichao |
author_facet | Lou, Mingxing Cao, Aihua Jin, Cuiyuan Mi, Kai Xiong, Xiyue Zeng, Zhaoyang Pan, Xu Qie, Jinlong Qiu, Shangfeng Niu, Yaofang Liang, Hao Liu, Yanping Chen, Lin Liu, Zhi Zhao, Qing Qiu, Xiyan Jin, Yuanxiang Sheng, Xiaoqi Hu, Zhibin Jin, Gulei Liu, Jingshi Liu, Xingyin Wang, Yichao |
author_sort | Lou, Mingxing |
collection | PubMed |
description | OBJECTIVE: Recent studies have provided insights into the gut microbiota in autism spectrum disorder (ASD); however, these studies were restricted owing to limited sampling at the unitary stage of childhood. Herein, we aimed to reveal developmental characteristics of gut microbiota in a large cohort of subjects with ASD combined with interindividual factors impacting gut microbiota. DESIGN: A large cohort of 773 subjects with ASD (aged 16 months to 19 years), 429 neurotypical (NT) development subjects (aged 11 months to 15 years) were emolyed to determine the dynamics change of gut microbiota across different ages using 16S rRNA sequencing. RESULT: In subjects with ASD, we observed a distinct but progressive deviation in the development of gut microbiota characterised by persistently decreased alpha diversity, early unsustainable immature microbiota, altered aboudance of 20 operational taxonomic units (OTUs), decreased taxon detection rate and 325 deregulated microbial metabolic functions with age-dependent patterns. We further revealed microbial relationships that have changed extensively in ASD before 3 years of age, which were associated with the severity of behaviour, sleep and GI symptoms in the ASD group. This analysis demonstrated that a signature of the combination of 2 OTUs, Veillonella and Enterobacteriaceae, and 17 microbial metabolic functions efficiently discriminated ASD from NT subjects in both the discovery (area under the curve (AUC)=0.86), and validation 1 (AUC=0.78), 2 (AUC=0.82) and 3 (AUC=0.67) sets. CONCLUSION: Our large cohort combined with clinical symptom analysis highlights the key regulator of gut microbiota in the pathogenesis of ASD and emphasises the importance of monitoring and targeting the gut microbiome in future clinical applications of ASD. |
format | Online Article Text |
id | pubmed-9279844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-92798442022-08-01 Deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder Lou, Mingxing Cao, Aihua Jin, Cuiyuan Mi, Kai Xiong, Xiyue Zeng, Zhaoyang Pan, Xu Qie, Jinlong Qiu, Shangfeng Niu, Yaofang Liang, Hao Liu, Yanping Chen, Lin Liu, Zhi Zhao, Qing Qiu, Xiyan Jin, Yuanxiang Sheng, Xiaoqi Hu, Zhibin Jin, Gulei Liu, Jingshi Liu, Xingyin Wang, Yichao Gut Gut Microbiota OBJECTIVE: Recent studies have provided insights into the gut microbiota in autism spectrum disorder (ASD); however, these studies were restricted owing to limited sampling at the unitary stage of childhood. Herein, we aimed to reveal developmental characteristics of gut microbiota in a large cohort of subjects with ASD combined with interindividual factors impacting gut microbiota. DESIGN: A large cohort of 773 subjects with ASD (aged 16 months to 19 years), 429 neurotypical (NT) development subjects (aged 11 months to 15 years) were emolyed to determine the dynamics change of gut microbiota across different ages using 16S rRNA sequencing. RESULT: In subjects with ASD, we observed a distinct but progressive deviation in the development of gut microbiota characterised by persistently decreased alpha diversity, early unsustainable immature microbiota, altered aboudance of 20 operational taxonomic units (OTUs), decreased taxon detection rate and 325 deregulated microbial metabolic functions with age-dependent patterns. We further revealed microbial relationships that have changed extensively in ASD before 3 years of age, which were associated with the severity of behaviour, sleep and GI symptoms in the ASD group. This analysis demonstrated that a signature of the combination of 2 OTUs, Veillonella and Enterobacteriaceae, and 17 microbial metabolic functions efficiently discriminated ASD from NT subjects in both the discovery (area under the curve (AUC)=0.86), and validation 1 (AUC=0.78), 2 (AUC=0.82) and 3 (AUC=0.67) sets. CONCLUSION: Our large cohort combined with clinical symptom analysis highlights the key regulator of gut microbiota in the pathogenesis of ASD and emphasises the importance of monitoring and targeting the gut microbiome in future clinical applications of ASD. BMJ Publishing Group 2022-08 2021-12-20 /pmc/articles/PMC9279844/ /pubmed/34930815 http://dx.doi.org/10.1136/gutjnl-2021-325115 Text en © Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Gut Microbiota Lou, Mingxing Cao, Aihua Jin, Cuiyuan Mi, Kai Xiong, Xiyue Zeng, Zhaoyang Pan, Xu Qie, Jinlong Qiu, Shangfeng Niu, Yaofang Liang, Hao Liu, Yanping Chen, Lin Liu, Zhi Zhao, Qing Qiu, Xiyan Jin, Yuanxiang Sheng, Xiaoqi Hu, Zhibin Jin, Gulei Liu, Jingshi Liu, Xingyin Wang, Yichao Deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder |
title | Deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder |
title_full | Deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder |
title_fullStr | Deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder |
title_full_unstemmed | Deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder |
title_short | Deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder |
title_sort | deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder |
topic | Gut Microbiota |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279844/ https://www.ncbi.nlm.nih.gov/pubmed/34930815 http://dx.doi.org/10.1136/gutjnl-2021-325115 |
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