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Prognostic score model based on six m6A‐related autophagy genes for predicting survival in esophageal squamous cell carcinoma

BACKGROUND: Prognostic signatures based on autophagy genes have been proposed for esophageal squamous cell carcinoma (ESCC). Autophagy genes are closely associated with m6A genes. Our purpose is to identify m6A‐related autophagy genes in ESCC and develop a survival prediction model. METHODS: Differe...

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Autores principales: Chen, Funan, Gong, Erxiu, Ma, Jun, Lin, Jiehuan, Wu, Canxing, Chen, Shanshan, Hu, Shuqiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279981/
https://www.ncbi.nlm.nih.gov/pubmed/35611939
http://dx.doi.org/10.1002/jcla.24507
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author Chen, Funan
Gong, Erxiu
Ma, Jun
Lin, Jiehuan
Wu, Canxing
Chen, Shanshan
Hu, Shuqiao
author_facet Chen, Funan
Gong, Erxiu
Ma, Jun
Lin, Jiehuan
Wu, Canxing
Chen, Shanshan
Hu, Shuqiao
author_sort Chen, Funan
collection PubMed
description BACKGROUND: Prognostic signatures based on autophagy genes have been proposed for esophageal squamous cell carcinoma (ESCC). Autophagy genes are closely associated with m6A genes. Our purpose is to identify m6A‐related autophagy genes in ESCC and develop a survival prediction model. METHODS: Differential expression analyses for m6A genes and autophagy genes were performed based on TCGA and HADd databases followed by constructing a co‐expression network. Uni‐variable Cox regression analysis was performed for m6A‐related autophagy genes. Using the optimal combination of feature genes by LASSO Cox regression model, a prognostic score (PS) model was developed and subsequently validated in an independent dataset. RESULTS: The differential expression of 13 m6A genes and 107 autophagy genes was observed between ESCC and normal samples. The co‐expression network contained 13 m6A genes and 96 autophagy genes. Of the 12 m6A‐related autophagy genes that were significantly related to survival, DAPK2, DIRAS3, EIF2AK3, ITPR1, MAP1LC3C, and TP53 were used to construct a PS model, which split the training set into two risk groups with significant different survival ratios (p = 0.015, 1‐year, 3‐year, and 5‐year AUC = 0.873, 0.840, and 0.829). Consistent results of GSE53625 dataset confirmed predictive ability of the model (p = 0.024, 1‐year, 3‐year, and 5‐year AUC = 0.793, 0.751, and 0.744). The six‐gene PS score was an independent prognostic factor from clinical factors (HR, 2.362; 95% CI, 1.390–7.064; p‐value = 0.012). CONCLUSION: Our study recommends 6 m6A‐related autophagy genes as promising prognostic biomarkers and develops a PS model to predict survival in ESCC.
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spelling pubmed-92799812022-07-15 Prognostic score model based on six m6A‐related autophagy genes for predicting survival in esophageal squamous cell carcinoma Chen, Funan Gong, Erxiu Ma, Jun Lin, Jiehuan Wu, Canxing Chen, Shanshan Hu, Shuqiao J Clin Lab Anal Research Articles BACKGROUND: Prognostic signatures based on autophagy genes have been proposed for esophageal squamous cell carcinoma (ESCC). Autophagy genes are closely associated with m6A genes. Our purpose is to identify m6A‐related autophagy genes in ESCC and develop a survival prediction model. METHODS: Differential expression analyses for m6A genes and autophagy genes were performed based on TCGA and HADd databases followed by constructing a co‐expression network. Uni‐variable Cox regression analysis was performed for m6A‐related autophagy genes. Using the optimal combination of feature genes by LASSO Cox regression model, a prognostic score (PS) model was developed and subsequently validated in an independent dataset. RESULTS: The differential expression of 13 m6A genes and 107 autophagy genes was observed between ESCC and normal samples. The co‐expression network contained 13 m6A genes and 96 autophagy genes. Of the 12 m6A‐related autophagy genes that were significantly related to survival, DAPK2, DIRAS3, EIF2AK3, ITPR1, MAP1LC3C, and TP53 were used to construct a PS model, which split the training set into two risk groups with significant different survival ratios (p = 0.015, 1‐year, 3‐year, and 5‐year AUC = 0.873, 0.840, and 0.829). Consistent results of GSE53625 dataset confirmed predictive ability of the model (p = 0.024, 1‐year, 3‐year, and 5‐year AUC = 0.793, 0.751, and 0.744). The six‐gene PS score was an independent prognostic factor from clinical factors (HR, 2.362; 95% CI, 1.390–7.064; p‐value = 0.012). CONCLUSION: Our study recommends 6 m6A‐related autophagy genes as promising prognostic biomarkers and develops a PS model to predict survival in ESCC. John Wiley and Sons Inc. 2022-05-25 /pmc/articles/PMC9279981/ /pubmed/35611939 http://dx.doi.org/10.1002/jcla.24507 Text en © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Chen, Funan
Gong, Erxiu
Ma, Jun
Lin, Jiehuan
Wu, Canxing
Chen, Shanshan
Hu, Shuqiao
Prognostic score model based on six m6A‐related autophagy genes for predicting survival in esophageal squamous cell carcinoma
title Prognostic score model based on six m6A‐related autophagy genes for predicting survival in esophageal squamous cell carcinoma
title_full Prognostic score model based on six m6A‐related autophagy genes for predicting survival in esophageal squamous cell carcinoma
title_fullStr Prognostic score model based on six m6A‐related autophagy genes for predicting survival in esophageal squamous cell carcinoma
title_full_unstemmed Prognostic score model based on six m6A‐related autophagy genes for predicting survival in esophageal squamous cell carcinoma
title_short Prognostic score model based on six m6A‐related autophagy genes for predicting survival in esophageal squamous cell carcinoma
title_sort prognostic score model based on six m6a‐related autophagy genes for predicting survival in esophageal squamous cell carcinoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279981/
https://www.ncbi.nlm.nih.gov/pubmed/35611939
http://dx.doi.org/10.1002/jcla.24507
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