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Generation of MiRNA sponge constructs targeting multiple MiRNAs
BACKGROUND: MicroRNAs(miRNAs) are key regulators of gene expression in plants, animals and some viruses. Hence, alteration of miRNA levels in cells or tissues is common for miRNA studies. Loss‐of‐function of miRNA can be achieved using antisense oligonucleotides, sponges and gene knockout models. ME...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279985/ https://www.ncbi.nlm.nih.gov/pubmed/35666624 http://dx.doi.org/10.1002/jcla.24527 |
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author | Jie, Junjin Liu, Danni Wang, Yifan Wu, Qiong Wu, Tingting Fang, Rong |
author_facet | Jie, Junjin Liu, Danni Wang, Yifan Wu, Qiong Wu, Tingting Fang, Rong |
author_sort | Jie, Junjin |
collection | PubMed |
description | BACKGROUND: MicroRNAs(miRNAs) are key regulators of gene expression in plants, animals and some viruses. Hence, alteration of miRNA levels in cells or tissues is common for miRNA studies. Loss‐of‐function of miRNA can be achieved using antisense oligonucleotides, sponges and gene knockout models. METHODS: Here, we showed an efficient, rational and economical way to construct multi‐targeted miRNA sponges with desired copies. Four copies of miRNA sponge are used as “building‐blocks”. RESULTS: These building‐blocks, which can target same miRNA or different miRNAs, are linked together through ligation. Each time of ligation can double the number of sponge copies. CONCLUSIONS: In this way, we constructed lentivirus vectors harboring sponges targeting miR‐21, miR‐31 and miR‐155 and the combination of two miRNA sponges can inhibit cancer cell growth significantly. |
format | Online Article Text |
id | pubmed-9279985 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92799852022-07-15 Generation of MiRNA sponge constructs targeting multiple MiRNAs Jie, Junjin Liu, Danni Wang, Yifan Wu, Qiong Wu, Tingting Fang, Rong J Clin Lab Anal Research Articles BACKGROUND: MicroRNAs(miRNAs) are key regulators of gene expression in plants, animals and some viruses. Hence, alteration of miRNA levels in cells or tissues is common for miRNA studies. Loss‐of‐function of miRNA can be achieved using antisense oligonucleotides, sponges and gene knockout models. METHODS: Here, we showed an efficient, rational and economical way to construct multi‐targeted miRNA sponges with desired copies. Four copies of miRNA sponge are used as “building‐blocks”. RESULTS: These building‐blocks, which can target same miRNA or different miRNAs, are linked together through ligation. Each time of ligation can double the number of sponge copies. CONCLUSIONS: In this way, we constructed lentivirus vectors harboring sponges targeting miR‐21, miR‐31 and miR‐155 and the combination of two miRNA sponges can inhibit cancer cell growth significantly. John Wiley and Sons Inc. 2022-06-06 /pmc/articles/PMC9279985/ /pubmed/35666624 http://dx.doi.org/10.1002/jcla.24527 Text en © 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Jie, Junjin Liu, Danni Wang, Yifan Wu, Qiong Wu, Tingting Fang, Rong Generation of MiRNA sponge constructs targeting multiple MiRNAs |
title | Generation of MiRNA sponge constructs targeting multiple MiRNAs
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title_full | Generation of MiRNA sponge constructs targeting multiple MiRNAs
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title_fullStr | Generation of MiRNA sponge constructs targeting multiple MiRNAs
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title_full_unstemmed | Generation of MiRNA sponge constructs targeting multiple MiRNAs
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title_short | Generation of MiRNA sponge constructs targeting multiple MiRNAs
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title_sort | generation of mirna sponge constructs targeting multiple mirnas |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279985/ https://www.ncbi.nlm.nih.gov/pubmed/35666624 http://dx.doi.org/10.1002/jcla.24527 |
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