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Influence of halloysite nanotubes on the efficiency of Asparaginase against mice Ehrlich solid carcinoma

Herein, the impact of the halloysite nanotubes to suppress the side effects of Asparaginase (ANase) cellular proliferation was investigated. Methods: A total of 100 adult male mice was employed. These mice were divided into four equal groups; Group 1 (control), Group 2 (ESC group) of a single dose o...

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Autores principales: Baharoon, B.M.M., Shaik, A.M., El-Hamidy, Salim M., Eid El-Araby, Rady, Batawi, Ashwaq H., Abdel Salam, Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280262/
https://www.ncbi.nlm.nih.gov/pubmed/35844382
http://dx.doi.org/10.1016/j.sjbs.2022.02.058
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author Baharoon, B.M.M.
Shaik, A.M.
El-Hamidy, Salim M.
Eid El-Araby, Rady
Batawi, Ashwaq H.
Abdel Salam, Mohamed
author_facet Baharoon, B.M.M.
Shaik, A.M.
El-Hamidy, Salim M.
Eid El-Araby, Rady
Batawi, Ashwaq H.
Abdel Salam, Mohamed
author_sort Baharoon, B.M.M.
collection PubMed
description Herein, the impact of the halloysite nanotubes to suppress the side effects of Asparaginase (ANase) cellular proliferation was investigated. Methods: A total of 100 adult male mice was employed. These mice were divided into four equal groups; Group 1 (control), Group 2 (ESC group) of a single dose of 0.15 ml Ehrlich cells (2 × 10(6)) intraperitoneal infusion(IP), Group 3 (ESC + ANase group) received six doses equal treatments of Intratumoral (IT) 0.07 ml Aspragnase (7 mg/kg) over two weeks. For two weeks, Group 4 (ESC + ASNase + HNTs) received an IT administration of 0.07 ml Asparaginase stocked on Halloysite nanotubes (HNTs) (30 mg/kg) three times per week. A blood specimen was collected, and the liver was removed to be investigated histologically. Results: TEM measurements for the Halloysite nanoclay showed their tubular cylindrical shape with a mean diameter of 50 nm and an average length of 1 μm, whereas The X-ray diffraction pattern of the Halloysite nanoclay showed their characteristic peaks. ESC increases the serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and bilirubin than control and other groups, even as albumin and total protein were decreasing. After using Halloysite Nanotube, the rates of these variables were enhanced up to 75%. The hepatocytes histological studies showed protection against Ehrlich Solid carcinoma-induced degenerative, necrotic, and inflammatory changes up to 70%. In conclusion, halloysite nanotubes have demonstrated effective removal of Ehrlich solid carcinoma in mice using an ASNase delivery system. It promoted the ASNase to inhibit the adverse effect of ANase's on the liver and remove the tumour cells.
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spelling pubmed-92802622022-07-15 Influence of halloysite nanotubes on the efficiency of Asparaginase against mice Ehrlich solid carcinoma Baharoon, B.M.M. Shaik, A.M. El-Hamidy, Salim M. Eid El-Araby, Rady Batawi, Ashwaq H. Abdel Salam, Mohamed Saudi J Biol Sci Original Article Herein, the impact of the halloysite nanotubes to suppress the side effects of Asparaginase (ANase) cellular proliferation was investigated. Methods: A total of 100 adult male mice was employed. These mice were divided into four equal groups; Group 1 (control), Group 2 (ESC group) of a single dose of 0.15 ml Ehrlich cells (2 × 10(6)) intraperitoneal infusion(IP), Group 3 (ESC + ANase group) received six doses equal treatments of Intratumoral (IT) 0.07 ml Aspragnase (7 mg/kg) over two weeks. For two weeks, Group 4 (ESC + ASNase + HNTs) received an IT administration of 0.07 ml Asparaginase stocked on Halloysite nanotubes (HNTs) (30 mg/kg) three times per week. A blood specimen was collected, and the liver was removed to be investigated histologically. Results: TEM measurements for the Halloysite nanoclay showed their tubular cylindrical shape with a mean diameter of 50 nm and an average length of 1 μm, whereas The X-ray diffraction pattern of the Halloysite nanoclay showed their characteristic peaks. ESC increases the serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and bilirubin than control and other groups, even as albumin and total protein were decreasing. After using Halloysite Nanotube, the rates of these variables were enhanced up to 75%. The hepatocytes histological studies showed protection against Ehrlich Solid carcinoma-induced degenerative, necrotic, and inflammatory changes up to 70%. In conclusion, halloysite nanotubes have demonstrated effective removal of Ehrlich solid carcinoma in mice using an ASNase delivery system. It promoted the ASNase to inhibit the adverse effect of ANase's on the liver and remove the tumour cells. Elsevier 2022-05 2022-03-04 /pmc/articles/PMC9280262/ /pubmed/35844382 http://dx.doi.org/10.1016/j.sjbs.2022.02.058 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Baharoon, B.M.M.
Shaik, A.M.
El-Hamidy, Salim M.
Eid El-Araby, Rady
Batawi, Ashwaq H.
Abdel Salam, Mohamed
Influence of halloysite nanotubes on the efficiency of Asparaginase against mice Ehrlich solid carcinoma
title Influence of halloysite nanotubes on the efficiency of Asparaginase against mice Ehrlich solid carcinoma
title_full Influence of halloysite nanotubes on the efficiency of Asparaginase against mice Ehrlich solid carcinoma
title_fullStr Influence of halloysite nanotubes on the efficiency of Asparaginase against mice Ehrlich solid carcinoma
title_full_unstemmed Influence of halloysite nanotubes on the efficiency of Asparaginase against mice Ehrlich solid carcinoma
title_short Influence of halloysite nanotubes on the efficiency of Asparaginase against mice Ehrlich solid carcinoma
title_sort influence of halloysite nanotubes on the efficiency of asparaginase against mice ehrlich solid carcinoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280262/
https://www.ncbi.nlm.nih.gov/pubmed/35844382
http://dx.doi.org/10.1016/j.sjbs.2022.02.058
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