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Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency()

Jmjd6 is a conserved nuclear protein which possesses histone arginine demethylation and lysyl hydroxylase activity. Previous studies have revealed that Jmjd6 is essential for cell differentiation and embryo development. However, the role of Jmjd6 in mammalian ES cell identity and reprogramming has b...

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Detalles Bibliográficos
Autores principales: Ji, Guanxu, Xiao, Xiaoxiao, Huang, Min, Wu, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280369/
https://www.ncbi.nlm.nih.gov/pubmed/35846449
http://dx.doi.org/10.1016/j.heliyon.2022.e09105
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author Ji, Guanxu
Xiao, Xiaoxiao
Huang, Min
Wu, Qiang
author_facet Ji, Guanxu
Xiao, Xiaoxiao
Huang, Min
Wu, Qiang
author_sort Ji, Guanxu
collection PubMed
description Jmjd6 is a conserved nuclear protein which possesses histone arginine demethylation and lysyl hydroxylase activity. Previous studies have revealed that Jmjd6 is essential for cell differentiation and embryo development. However, the role of Jmjd6 in mammalian ES cell identity and reprogramming has been unclear. Here we report that depletion of Jmjd6 not only results in downregulation of pluripotency genes but also is implicated in apoptosis, glycolysis, cell cycle and protein hydroxylation. We also revealed the reduction of BrdU incorporation in Jmjd6 depleted cells. Reprogramming efficiency of MEFs can be enhanced with Jmjd6 overexpression while the efficiency was reduced upon Jmjd6 depletion. Together, these results suggest that Jmjd6 can regulate ES cell homeostasis and enhance somatic cell reprogramming.
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spelling pubmed-92803692022-07-15 Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency() Ji, Guanxu Xiao, Xiaoxiao Huang, Min Wu, Qiang Heliyon Research Article Jmjd6 is a conserved nuclear protein which possesses histone arginine demethylation and lysyl hydroxylase activity. Previous studies have revealed that Jmjd6 is essential for cell differentiation and embryo development. However, the role of Jmjd6 in mammalian ES cell identity and reprogramming has been unclear. Here we report that depletion of Jmjd6 not only results in downregulation of pluripotency genes but also is implicated in apoptosis, glycolysis, cell cycle and protein hydroxylation. We also revealed the reduction of BrdU incorporation in Jmjd6 depleted cells. Reprogramming efficiency of MEFs can be enhanced with Jmjd6 overexpression while the efficiency was reduced upon Jmjd6 depletion. Together, these results suggest that Jmjd6 can regulate ES cell homeostasis and enhance somatic cell reprogramming. Elsevier 2022-03-15 /pmc/articles/PMC9280369/ /pubmed/35846449 http://dx.doi.org/10.1016/j.heliyon.2022.e09105 Text en © 2022 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Ji, Guanxu
Xiao, Xiaoxiao
Huang, Min
Wu, Qiang
Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency()
title Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency()
title_full Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency()
title_fullStr Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency()
title_full_unstemmed Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency()
title_short Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency()
title_sort jmjd6 regulates es cell homeostasis and enhances reprogramming efficiency()
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280369/
https://www.ncbi.nlm.nih.gov/pubmed/35846449
http://dx.doi.org/10.1016/j.heliyon.2022.e09105
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