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A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro

The gut microbiota is engaged in multiple interactions affecting host health. Bacteriocins showed the ability of impeding the growth of intestinal pathogenic bacteria and modulating gut microbiota in animals. Few studies have also discovered their regulation on human intestinal flora using an in vit...

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Autores principales: Pu, Jiaqian, Hang, Shuting, Liu, Manman, Chen, Ziqi, Xiong, Jiayi, Li, Yongquan, Wu, Hongchen, Zhao, Xiaodan, Liu, Shuxun, Gu, Qing, Li, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280423/
https://www.ncbi.nlm.nih.gov/pubmed/35845772
http://dx.doi.org/10.3389/fnut.2022.877948
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author Pu, Jiaqian
Hang, Shuting
Liu, Manman
Chen, Ziqi
Xiong, Jiayi
Li, Yongquan
Wu, Hongchen
Zhao, Xiaodan
Liu, Shuxun
Gu, Qing
Li, Ping
author_facet Pu, Jiaqian
Hang, Shuting
Liu, Manman
Chen, Ziqi
Xiong, Jiayi
Li, Yongquan
Wu, Hongchen
Zhao, Xiaodan
Liu, Shuxun
Gu, Qing
Li, Ping
author_sort Pu, Jiaqian
collection PubMed
description The gut microbiota is engaged in multiple interactions affecting host health. Bacteriocins showed the ability of impeding the growth of intestinal pathogenic bacteria and modulating gut microbiota in animals. Few studies have also discovered their regulation on human intestinal flora using an in vitro simulated system. However, little is known about their effect on gut microbiota of different enterotypes of human. This work evaluated the modification of the gut microbiota of two enterotypes (ET B and ET P) by the class IIb bacteriocin plantaricin NC8 (PLNC8) by using an in vitro fermentation model of the intestine. Gas chromatography results revealed that PLNC8 had no influence on the gut microbiota’s production of short-chain fatty acids in the subjects’ samples. PLNC8 lowered the Shannon index of ET B’ gut microbiota and the Simpson index of ET P’ gut microbiota, according to 16S rDNA sequencing. In ET B, PLNC8 enhanced the abundance of Bacteroides, Bifidobacterium, Megamonas, Escherichia-Shigella, Parabacteroides, and Lactobacillus while decreasing the abundance of Streptococcus. Prevotella_9, Bifidobacterium, Escherichia-Shigella, Mitsuokella, and Collinsella were found more abundant in ET P. The current study adds to our understanding of the impact of PLNC8 on the human gut microbiota and lays the groundwork for future research into PLNC8’s effects on human intestinal disease.
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spelling pubmed-92804232022-07-15 A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro Pu, Jiaqian Hang, Shuting Liu, Manman Chen, Ziqi Xiong, Jiayi Li, Yongquan Wu, Hongchen Zhao, Xiaodan Liu, Shuxun Gu, Qing Li, Ping Front Nutr Nutrition The gut microbiota is engaged in multiple interactions affecting host health. Bacteriocins showed the ability of impeding the growth of intestinal pathogenic bacteria and modulating gut microbiota in animals. Few studies have also discovered their regulation on human intestinal flora using an in vitro simulated system. However, little is known about their effect on gut microbiota of different enterotypes of human. This work evaluated the modification of the gut microbiota of two enterotypes (ET B and ET P) by the class IIb bacteriocin plantaricin NC8 (PLNC8) by using an in vitro fermentation model of the intestine. Gas chromatography results revealed that PLNC8 had no influence on the gut microbiota’s production of short-chain fatty acids in the subjects’ samples. PLNC8 lowered the Shannon index of ET B’ gut microbiota and the Simpson index of ET P’ gut microbiota, according to 16S rDNA sequencing. In ET B, PLNC8 enhanced the abundance of Bacteroides, Bifidobacterium, Megamonas, Escherichia-Shigella, Parabacteroides, and Lactobacillus while decreasing the abundance of Streptococcus. Prevotella_9, Bifidobacterium, Escherichia-Shigella, Mitsuokella, and Collinsella were found more abundant in ET P. The current study adds to our understanding of the impact of PLNC8 on the human gut microbiota and lays the groundwork for future research into PLNC8’s effects on human intestinal disease. Frontiers Media S.A. 2022-06-30 /pmc/articles/PMC9280423/ /pubmed/35845772 http://dx.doi.org/10.3389/fnut.2022.877948 Text en Copyright © 2022 Pu, Hang, Liu, Chen, Xiong, Li, Wu, Zhao, Liu, Gu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Pu, Jiaqian
Hang, Shuting
Liu, Manman
Chen, Ziqi
Xiong, Jiayi
Li, Yongquan
Wu, Hongchen
Zhao, Xiaodan
Liu, Shuxun
Gu, Qing
Li, Ping
A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro
title A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro
title_full A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro
title_fullStr A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro
title_full_unstemmed A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro
title_short A Class IIb Bacteriocin Plantaricin NC8 Modulates Gut Microbiota of Different Enterotypes in vitro
title_sort class iib bacteriocin plantaricin nc8 modulates gut microbiota of different enterotypes in vitro
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280423/
https://www.ncbi.nlm.nih.gov/pubmed/35845772
http://dx.doi.org/10.3389/fnut.2022.877948
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