An Altered Metabolism in Leukocytes Showing in vitro igG Memory From SARS-CoV-2-Infected Patients

Coronavirus disease 2019 (COVID 19) is a systemic infection that exerts a significant impact on cell metabolism. In this study we performed metabolomic profiling of 41 in vitro cultures of peripheral blood mononuclear cells (PBMC), 17 of which displayed IgG memory for spike-S1 antigen 60–90 days aft...

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Autores principales: Fanelli, G., Gevi, F., Zarletti, G., Tiberi, M., De Molfetta, V., Scapigliati, G., Timperio, A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280710/
https://www.ncbi.nlm.nih.gov/pubmed/35847976
http://dx.doi.org/10.3389/fmolb.2022.894207
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author Fanelli, G.
Gevi, F.
Zarletti, G.
Tiberi, M.
De Molfetta, V.
Scapigliati, G.
Timperio, A. M.
author_facet Fanelli, G.
Gevi, F.
Zarletti, G.
Tiberi, M.
De Molfetta, V.
Scapigliati, G.
Timperio, A. M.
author_sort Fanelli, G.
collection PubMed
description Coronavirus disease 2019 (COVID 19) is a systemic infection that exerts a significant impact on cell metabolism. In this study we performed metabolomic profiling of 41 in vitro cultures of peripheral blood mononuclear cells (PBMC), 17 of which displayed IgG memory for spike-S1 antigen 60–90 days after infection. By using mass spectrometry analysis, a significant up-regulation of S-adenosyl-Homocysteine, Sarcosine and Arginine was found in leukocytes showing IgG memory. These metabolites are known to be involved in physiological recovery from viral infections and immune activities, and our findings might represent a novel and easy measure that could be of help in understanding SARS-Cov-2 effects on leukocytes.
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spelling pubmed-92807102022-07-15 An Altered Metabolism in Leukocytes Showing in vitro igG Memory From SARS-CoV-2-Infected Patients Fanelli, G. Gevi, F. Zarletti, G. Tiberi, M. De Molfetta, V. Scapigliati, G. Timperio, A. M. Front Mol Biosci Molecular Biosciences Coronavirus disease 2019 (COVID 19) is a systemic infection that exerts a significant impact on cell metabolism. In this study we performed metabolomic profiling of 41 in vitro cultures of peripheral blood mononuclear cells (PBMC), 17 of which displayed IgG memory for spike-S1 antigen 60–90 days after infection. By using mass spectrometry analysis, a significant up-regulation of S-adenosyl-Homocysteine, Sarcosine and Arginine was found in leukocytes showing IgG memory. These metabolites are known to be involved in physiological recovery from viral infections and immune activities, and our findings might represent a novel and easy measure that could be of help in understanding SARS-Cov-2 effects on leukocytes. Frontiers Media S.A. 2022-06-30 /pmc/articles/PMC9280710/ /pubmed/35847976 http://dx.doi.org/10.3389/fmolb.2022.894207 Text en Copyright © 2022 Fanelli, Gevi, Zarletti, Tiberi, De Molfetta, Scapigliati and Timperio. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Fanelli, G.
Gevi, F.
Zarletti, G.
Tiberi, M.
De Molfetta, V.
Scapigliati, G.
Timperio, A. M.
An Altered Metabolism in Leukocytes Showing in vitro igG Memory From SARS-CoV-2-Infected Patients
title An Altered Metabolism in Leukocytes Showing in vitro igG Memory From SARS-CoV-2-Infected Patients
title_full An Altered Metabolism in Leukocytes Showing in vitro igG Memory From SARS-CoV-2-Infected Patients
title_fullStr An Altered Metabolism in Leukocytes Showing in vitro igG Memory From SARS-CoV-2-Infected Patients
title_full_unstemmed An Altered Metabolism in Leukocytes Showing in vitro igG Memory From SARS-CoV-2-Infected Patients
title_short An Altered Metabolism in Leukocytes Showing in vitro igG Memory From SARS-CoV-2-Infected Patients
title_sort altered metabolism in leukocytes showing in vitro igg memory from sars-cov-2-infected patients
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280710/
https://www.ncbi.nlm.nih.gov/pubmed/35847976
http://dx.doi.org/10.3389/fmolb.2022.894207
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