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Generation of genome-edited dogs by somatic cell nuclear transfer
BACKGROUND: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease. RESULTS: We constructed a CRISPR-Cas9 vector targeting cani...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281017/ https://www.ncbi.nlm.nih.gov/pubmed/35831828 http://dx.doi.org/10.1186/s12896-022-00749-3 |
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author | Kim, Dong-Ern Lee, Ji-Hye Ji, Kuk-Bin Park, Kang-Sun Kil, Tae-Young Koo, Okjae Kim, Min-Kyu |
author_facet | Kim, Dong-Ern Lee, Ji-Hye Ji, Kuk-Bin Park, Kang-Sun Kil, Tae-Young Koo, Okjae Kim, Min-Kyu |
author_sort | Kim, Dong-Ern |
collection | PubMed |
description | BACKGROUND: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease. RESULTS: We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed. CONCLUSION: SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12896-022-00749-3. |
format | Online Article Text |
id | pubmed-9281017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92810172022-07-15 Generation of genome-edited dogs by somatic cell nuclear transfer Kim, Dong-Ern Lee, Ji-Hye Ji, Kuk-Bin Park, Kang-Sun Kil, Tae-Young Koo, Okjae Kim, Min-Kyu BMC Biotechnol Research BACKGROUND: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease. RESULTS: We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection. We performed canine SCNT using genome-edited fibroblasts and successfully generated two genome-edited dogs. Both genome-edited dogs had insertion-deletion mutations at the target locus, and DJ-1 expression was either downregulated or completely repressed. CONCLUSION: SCNT successfully produced genome-edited dogs by using the CRISPR-Cas9 system for the first time. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12896-022-00749-3. BioMed Central 2022-07-13 /pmc/articles/PMC9281017/ /pubmed/35831828 http://dx.doi.org/10.1186/s12896-022-00749-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Kim, Dong-Ern Lee, Ji-Hye Ji, Kuk-Bin Park, Kang-Sun Kil, Tae-Young Koo, Okjae Kim, Min-Kyu Generation of genome-edited dogs by somatic cell nuclear transfer |
title | Generation of genome-edited dogs by somatic cell nuclear transfer |
title_full | Generation of genome-edited dogs by somatic cell nuclear transfer |
title_fullStr | Generation of genome-edited dogs by somatic cell nuclear transfer |
title_full_unstemmed | Generation of genome-edited dogs by somatic cell nuclear transfer |
title_short | Generation of genome-edited dogs by somatic cell nuclear transfer |
title_sort | generation of genome-edited dogs by somatic cell nuclear transfer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281017/ https://www.ncbi.nlm.nih.gov/pubmed/35831828 http://dx.doi.org/10.1186/s12896-022-00749-3 |
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