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An association between a positive direct antiglobulin test and HLA-DR12 in COVID-19
SARS-CoV-2 infection has been reported to be associated with a positive direct antiglobulin test (DAT). In this study, an analysis of 40 consecutive coronavirus disease 2019 (COVID-19) cases from December 2020 to September 2021 in Japan revealed that patients of 70 years and over were predisposed to...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281373/ https://www.ncbi.nlm.nih.gov/pubmed/35833981 http://dx.doi.org/10.1007/s00277-022-04921-9 |
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author | Matsuura, Hideaki Fujii, Sumie Matsui, Yusuke Sugiura, Yukari Akiyama, Hidehiko Miura, Yasuo |
author_facet | Matsuura, Hideaki Fujii, Sumie Matsui, Yusuke Sugiura, Yukari Akiyama, Hidehiko Miura, Yasuo |
author_sort | Matsuura, Hideaki |
collection | PubMed |
description | SARS-CoV-2 infection has been reported to be associated with a positive direct antiglobulin test (DAT). In this study, an analysis of 40 consecutive coronavirus disease 2019 (COVID-19) cases from December 2020 to September 2021 in Japan revealed that patients of 70 years and over were predisposed to a positive DAT. DAT positivity was related to a decrease in the hemoglobin level. Anemia in DAT-positive COVID-19 patients was attributed to hemolysis, which was corroborated by high reticulocyte counts and an increase in the red blood cell distribution width. Human leukocyte antigen (HLA)-DRB1*12:01 and DRB1*12:02 were exclusively found in DAT-positive COVID-19 patients. In silico assays for the Spike protein of SARS-CoV-2 predicted several common core peptides that met the criteria for a B cell epitope and strong binding to both HLA-DRB1*12:01 and DRB1*12:02. Among these peptides, the amino acids sequence TSNFR, which is found within the S1 subunit of SARS-CoV-2 Spike protein, is shared by human blood group antigen Rhesus (Rh) CE polypeptides. In vitro analysis showed that the expression of HLA-DR in CD4(+) T cells and CD8(+) T cells from a DAT-positive patient was increased after pulsation with TSNFR-sequence-containing peptides. In summary, positive DAT is related to enhanced anemia and to HLA-DR12 in the Japanese population. A peptide sequence within SARS-CoV-2 Spike protein may act as an epitope for IgG binding to RBCs in DAT-positive COVID-19 patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00277-022-04921-9. |
format | Online Article Text |
id | pubmed-9281373 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-92813732022-07-15 An association between a positive direct antiglobulin test and HLA-DR12 in COVID-19 Matsuura, Hideaki Fujii, Sumie Matsui, Yusuke Sugiura, Yukari Akiyama, Hidehiko Miura, Yasuo Ann Hematol Original Article SARS-CoV-2 infection has been reported to be associated with a positive direct antiglobulin test (DAT). In this study, an analysis of 40 consecutive coronavirus disease 2019 (COVID-19) cases from December 2020 to September 2021 in Japan revealed that patients of 70 years and over were predisposed to a positive DAT. DAT positivity was related to a decrease in the hemoglobin level. Anemia in DAT-positive COVID-19 patients was attributed to hemolysis, which was corroborated by high reticulocyte counts and an increase in the red blood cell distribution width. Human leukocyte antigen (HLA)-DRB1*12:01 and DRB1*12:02 were exclusively found in DAT-positive COVID-19 patients. In silico assays for the Spike protein of SARS-CoV-2 predicted several common core peptides that met the criteria for a B cell epitope and strong binding to both HLA-DRB1*12:01 and DRB1*12:02. Among these peptides, the amino acids sequence TSNFR, which is found within the S1 subunit of SARS-CoV-2 Spike protein, is shared by human blood group antigen Rhesus (Rh) CE polypeptides. In vitro analysis showed that the expression of HLA-DR in CD4(+) T cells and CD8(+) T cells from a DAT-positive patient was increased after pulsation with TSNFR-sequence-containing peptides. In summary, positive DAT is related to enhanced anemia and to HLA-DR12 in the Japanese population. A peptide sequence within SARS-CoV-2 Spike protein may act as an epitope for IgG binding to RBCs in DAT-positive COVID-19 patients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00277-022-04921-9. Springer Berlin Heidelberg 2022-07-14 2022 /pmc/articles/PMC9281373/ /pubmed/35833981 http://dx.doi.org/10.1007/s00277-022-04921-9 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Matsuura, Hideaki Fujii, Sumie Matsui, Yusuke Sugiura, Yukari Akiyama, Hidehiko Miura, Yasuo An association between a positive direct antiglobulin test and HLA-DR12 in COVID-19 |
title | An association between a positive direct antiglobulin test and HLA-DR12 in COVID-19 |
title_full | An association between a positive direct antiglobulin test and HLA-DR12 in COVID-19 |
title_fullStr | An association between a positive direct antiglobulin test and HLA-DR12 in COVID-19 |
title_full_unstemmed | An association between a positive direct antiglobulin test and HLA-DR12 in COVID-19 |
title_short | An association between a positive direct antiglobulin test and HLA-DR12 in COVID-19 |
title_sort | association between a positive direct antiglobulin test and hla-dr12 in covid-19 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281373/ https://www.ncbi.nlm.nih.gov/pubmed/35833981 http://dx.doi.org/10.1007/s00277-022-04921-9 |
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