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Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management

Coronavirus Disease 19 (COVID-19) caused by the SARS-CoV-2 virus remains a global pandemic having a serious impact on national economies and healthcare infrastructure. Accurate infection detection protocols are key to policy guidance and decision making. In this pilot study, we compared single versu...

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Autores principales: Webb, William R., Thapa, Gauri, Tirnoveanu, Alice, Kallu, Sabrina, Loo Jin Yi, Charlene, Shah, Nirali, Macari, Joseph, Mitchell, Sadie, Fagg, Graham J., Jeremiah, Rachael N., Theminimulle, Sandiya, Vuono, Romina, Mylona, Athina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282535/
https://www.ncbi.nlm.nih.gov/pubmed/35834476
http://dx.doi.org/10.1371/journal.pone.0269883
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author Webb, William R.
Thapa, Gauri
Tirnoveanu, Alice
Kallu, Sabrina
Loo Jin Yi, Charlene
Shah, Nirali
Macari, Joseph
Mitchell, Sadie
Fagg, Graham J.
Jeremiah, Rachael N.
Theminimulle, Sandiya
Vuono, Romina
Mylona, Athina
author_facet Webb, William R.
Thapa, Gauri
Tirnoveanu, Alice
Kallu, Sabrina
Loo Jin Yi, Charlene
Shah, Nirali
Macari, Joseph
Mitchell, Sadie
Fagg, Graham J.
Jeremiah, Rachael N.
Theminimulle, Sandiya
Vuono, Romina
Mylona, Athina
author_sort Webb, William R.
collection PubMed
description Coronavirus Disease 19 (COVID-19) caused by the SARS-CoV-2 virus remains a global pandemic having a serious impact on national economies and healthcare infrastructure. Accurate infection detection protocols are key to policy guidance and decision making. In this pilot study, we compared single versus replicate PCR testing for effective and accurate SARS-CoV-2 infection detection. One-Step Real-Time RT-PCR was employed for the detection of SARS-CoV-2 RNA isolated from individual nasopharyngeal swabs. A total of 10,014 swabs, sampled from the general public (hospital admissions, A&E, elective surgeries, cancer patients, care home residents and healthcare staff), were tested using standard replicate testing. Our analysis demonstrates that approximately 19% of SARS-CoV-2 infected individuals would have been reported as false negative if single sample Real-Time PCR testing was used. Therefore, two replicate tests can substantially decrease the risk of false negative reporting and reduce hospital and community infection rates. As the number of variants of concern increases, we believe that replicate testing is an essential consideration for effective SARS-CoV-2 infection detection and prevention of further outbreaks. A strategic approach limiting the number of missed infections is crucial in controlling the rise of new SARS-CoV-2 variants as well as the management of future pandemics.
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spelling pubmed-92825352022-07-15 Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management Webb, William R. Thapa, Gauri Tirnoveanu, Alice Kallu, Sabrina Loo Jin Yi, Charlene Shah, Nirali Macari, Joseph Mitchell, Sadie Fagg, Graham J. Jeremiah, Rachael N. Theminimulle, Sandiya Vuono, Romina Mylona, Athina PLoS One Research Article Coronavirus Disease 19 (COVID-19) caused by the SARS-CoV-2 virus remains a global pandemic having a serious impact on national economies and healthcare infrastructure. Accurate infection detection protocols are key to policy guidance and decision making. In this pilot study, we compared single versus replicate PCR testing for effective and accurate SARS-CoV-2 infection detection. One-Step Real-Time RT-PCR was employed for the detection of SARS-CoV-2 RNA isolated from individual nasopharyngeal swabs. A total of 10,014 swabs, sampled from the general public (hospital admissions, A&E, elective surgeries, cancer patients, care home residents and healthcare staff), were tested using standard replicate testing. Our analysis demonstrates that approximately 19% of SARS-CoV-2 infected individuals would have been reported as false negative if single sample Real-Time PCR testing was used. Therefore, two replicate tests can substantially decrease the risk of false negative reporting and reduce hospital and community infection rates. As the number of variants of concern increases, we believe that replicate testing is an essential consideration for effective SARS-CoV-2 infection detection and prevention of further outbreaks. A strategic approach limiting the number of missed infections is crucial in controlling the rise of new SARS-CoV-2 variants as well as the management of future pandemics. Public Library of Science 2022-07-14 /pmc/articles/PMC9282535/ /pubmed/35834476 http://dx.doi.org/10.1371/journal.pone.0269883 Text en © 2022 Webb et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Webb, William R.
Thapa, Gauri
Tirnoveanu, Alice
Kallu, Sabrina
Loo Jin Yi, Charlene
Shah, Nirali
Macari, Joseph
Mitchell, Sadie
Fagg, Graham J.
Jeremiah, Rachael N.
Theminimulle, Sandiya
Vuono, Romina
Mylona, Athina
Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management
title Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management
title_full Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management
title_fullStr Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management
title_full_unstemmed Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management
title_short Single vs replicate Real-Time PCR SARS-CoV-2 testing: Lessons learned for effective pandemic management
title_sort single vs replicate real-time pcr sars-cov-2 testing: lessons learned for effective pandemic management
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9282535/
https://www.ncbi.nlm.nih.gov/pubmed/35834476
http://dx.doi.org/10.1371/journal.pone.0269883
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