Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas

Three new bacterial strains, WHY3(T), WH131(T), and WH158(T), were isolated and described from the hemolymph of the Pacific oyster Crassostrea gigas utilizing polyphasic taxonomic techniques. The 16S rRNA gene sequence analysis revealed that strain WHY3(T) was a member of the genus Winogradskyella,...

Descripción completa

Detalles Bibliográficos
Autores principales: Pira, Hani, Risdian, Chandra, Müsken, Mathias, Schupp, Peter J., Wink, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283347/
https://www.ncbi.nlm.nih.gov/pubmed/35835967
http://dx.doi.org/10.1007/s00203-022-03099-y
_version_ 1784747291733655552
author Pira, Hani
Risdian, Chandra
Müsken, Mathias
Schupp, Peter J.
Wink, Joachim
author_facet Pira, Hani
Risdian, Chandra
Müsken, Mathias
Schupp, Peter J.
Wink, Joachim
author_sort Pira, Hani
collection PubMed
description Three new bacterial strains, WHY3(T), WH131(T), and WH158(T), were isolated and described from the hemolymph of the Pacific oyster Crassostrea gigas utilizing polyphasic taxonomic techniques. The 16S rRNA gene sequence analysis revealed that strain WHY3(T) was a member of the genus Winogradskyella, whereas strains WHI31(T) and WH158(T) were members of the genus Erythrobacter. According to the polygenomic study the three strains formed individual lineages with strong bootstrap support. The comparison of dDDH-and ANI values, percentage of conserved proteins (POCP), and average amino acid identity (AAl) between the three strains and their relatives established that the three strains represented two separate genera. Menaquinone-6 was reported as the major respiratory quinone in strain WHY3(T) and Ubiquinone-10 for strains WH131(T) and WH158(T), respectively. The major cellular fatty acids for strain WHY3(T) were C(15:0), anteiso-C(15:1) ω7c, iso-C(15:0), C(16:1)ω7c. The major cellular fatty acids for strains WH131(T) and WH158(T) were C(14:0)2-OH and t(18:1)ω12 for WH131(T) and C(17:0,) and C(18:1)ω7c for strain WH158(T). Positive Sudan Black B staining Indicated the presence of polyhydroxyalkanoic acid granules for strains WH131(T) and WH158(T) but not for strain WHY3(T). The DNA G + C contents of strains WHY3(T), WH131(T) and WH158(T) were 34.4, 59.7 and 56.6%, respectively. Gene clusters predicted some important genes involved in the bioremediation process. Due to the accomplishment of polyphasic taxonomy, we propose three novel species Winogradskyella luteola sp.nov. (type strain WHY3(T) = DSM 111804(T) = NCCB 100833(T)), Erythrobacter ani sp.nov. (WH131(T) = DSM 112099(T) = NCCB 100824(T)) and Erythrobacter crassostrea sp.nov. (WH158(T) = DSM 112102(T) = NCCB 100877(T)). SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00203-022-03099-y.
format Online
Article
Text
id pubmed-9283347
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-92833472022-07-16 Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas Pira, Hani Risdian, Chandra Müsken, Mathias Schupp, Peter J. Wink, Joachim Arch Microbiol Original Paper Three new bacterial strains, WHY3(T), WH131(T), and WH158(T), were isolated and described from the hemolymph of the Pacific oyster Crassostrea gigas utilizing polyphasic taxonomic techniques. The 16S rRNA gene sequence analysis revealed that strain WHY3(T) was a member of the genus Winogradskyella, whereas strains WHI31(T) and WH158(T) were members of the genus Erythrobacter. According to the polygenomic study the three strains formed individual lineages with strong bootstrap support. The comparison of dDDH-and ANI values, percentage of conserved proteins (POCP), and average amino acid identity (AAl) between the three strains and their relatives established that the three strains represented two separate genera. Menaquinone-6 was reported as the major respiratory quinone in strain WHY3(T) and Ubiquinone-10 for strains WH131(T) and WH158(T), respectively. The major cellular fatty acids for strain WHY3(T) were C(15:0), anteiso-C(15:1) ω7c, iso-C(15:0), C(16:1)ω7c. The major cellular fatty acids for strains WH131(T) and WH158(T) were C(14:0)2-OH and t(18:1)ω12 for WH131(T) and C(17:0,) and C(18:1)ω7c for strain WH158(T). Positive Sudan Black B staining Indicated the presence of polyhydroxyalkanoic acid granules for strains WH131(T) and WH158(T) but not for strain WHY3(T). The DNA G + C contents of strains WHY3(T), WH131(T) and WH158(T) were 34.4, 59.7 and 56.6%, respectively. Gene clusters predicted some important genes involved in the bioremediation process. Due to the accomplishment of polyphasic taxonomy, we propose three novel species Winogradskyella luteola sp.nov. (type strain WHY3(T) = DSM 111804(T) = NCCB 100833(T)), Erythrobacter ani sp.nov. (WH131(T) = DSM 112099(T) = NCCB 100824(T)) and Erythrobacter crassostrea sp.nov. (WH158(T) = DSM 112102(T) = NCCB 100877(T)). SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00203-022-03099-y. Springer Berlin Heidelberg 2022-07-14 2022 /pmc/articles/PMC9283347/ /pubmed/35835967 http://dx.doi.org/10.1007/s00203-022-03099-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Pira, Hani
Risdian, Chandra
Müsken, Mathias
Schupp, Peter J.
Wink, Joachim
Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas
title Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas
title_full Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas
title_fullStr Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas
title_full_unstemmed Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas
title_short Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas
title_sort winogradskyella luteola sp.nov., erythrobacter ani sp. nov., and erythrobacter crassostrea sp.nov., isolated from the hemolymph of the pacific oyster crassostrea gigas
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283347/
https://www.ncbi.nlm.nih.gov/pubmed/35835967
http://dx.doi.org/10.1007/s00203-022-03099-y
work_keys_str_mv AT pirahani winogradskyellaluteolaspnoverythrobacteranispnovanderythrobactercrassostreaspnovisolatedfromthehemolymphofthepacificoystercrassostreagigas
AT risdianchandra winogradskyellaluteolaspnoverythrobacteranispnovanderythrobactercrassostreaspnovisolatedfromthehemolymphofthepacificoystercrassostreagigas
AT muskenmathias winogradskyellaluteolaspnoverythrobacteranispnovanderythrobactercrassostreaspnovisolatedfromthehemolymphofthepacificoystercrassostreagigas
AT schupppeterj winogradskyellaluteolaspnoverythrobacteranispnovanderythrobactercrassostreaspnovisolatedfromthehemolymphofthepacificoystercrassostreagigas
AT winkjoachim winogradskyellaluteolaspnoverythrobacteranispnovanderythrobactercrassostreaspnovisolatedfromthehemolymphofthepacificoystercrassostreagigas

Ejemplares similares