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Test performance data demonstrates utility of a cattle DIVA skin test reagent (DST-F) compatible with BCG vaccination
Bacillus Calmette-Guérin (BCG), an attenuated strain of Mycobacterium bovis (M. bovis), is the lead candidate vaccine for control of bovine tuberculosis (TB) in cattle. However, BCG vaccination sensitises cattle to bovine tuberculin, thus compromising the use of the current bovine TB surveillance te...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283413/ https://www.ncbi.nlm.nih.gov/pubmed/35835806 http://dx.doi.org/10.1038/s41598-022-16092-8 |
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author | Jones, Gareth J. Konold, Timm Hurley, Shellene Holder, Tom Steinbach, Sabine Coad, Mick Neil Wedlock, D. Buddle, Bryce M. Singh, Mahavir Martin Vordermeier, H. |
author_facet | Jones, Gareth J. Konold, Timm Hurley, Shellene Holder, Tom Steinbach, Sabine Coad, Mick Neil Wedlock, D. Buddle, Bryce M. Singh, Mahavir Martin Vordermeier, H. |
author_sort | Jones, Gareth J. |
collection | PubMed |
description | Bacillus Calmette-Guérin (BCG), an attenuated strain of Mycobacterium bovis (M. bovis), is the lead candidate vaccine for control of bovine tuberculosis (TB) in cattle. However, BCG vaccination sensitises cattle to bovine tuberculin, thus compromising the use of the current bovine TB surveillance tests. To address this, we have developed a diagnostic skin test that is not compromised by BCG vaccination and is able to detect BCG vaccinated animals that subsequently develop bovine TB following exposure to M. bovis. Building on previous work using ‘in house’ formulated protein cocktail reagents, we herein present test performance data for a single fusion protein (DST-F) containing the mycobacterial antigens ESAT-6, CFP-10 and Rv3615c formulated as a ‘ready to use’ reagent by a commercial manufacturer. Our results demonstrate that, unlike tuberculin reagents, a diagnostic skin test using DST-F maintained high specificity in BCG vaccinated animals. Furthermore, the DST-F skin test demonstrated a high relative sensitivity in identifying M. bovis infected animals, including those where BCG vaccination failed to prevent bovine TB pathology following experimental exposure to M. bovis. The DST-F is currently undergoing field trials in Great Britain to support its licensure and commercialisation. |
format | Online Article Text |
id | pubmed-9283413 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-92834132022-07-16 Test performance data demonstrates utility of a cattle DIVA skin test reagent (DST-F) compatible with BCG vaccination Jones, Gareth J. Konold, Timm Hurley, Shellene Holder, Tom Steinbach, Sabine Coad, Mick Neil Wedlock, D. Buddle, Bryce M. Singh, Mahavir Martin Vordermeier, H. Sci Rep Article Bacillus Calmette-Guérin (BCG), an attenuated strain of Mycobacterium bovis (M. bovis), is the lead candidate vaccine for control of bovine tuberculosis (TB) in cattle. However, BCG vaccination sensitises cattle to bovine tuberculin, thus compromising the use of the current bovine TB surveillance tests. To address this, we have developed a diagnostic skin test that is not compromised by BCG vaccination and is able to detect BCG vaccinated animals that subsequently develop bovine TB following exposure to M. bovis. Building on previous work using ‘in house’ formulated protein cocktail reagents, we herein present test performance data for a single fusion protein (DST-F) containing the mycobacterial antigens ESAT-6, CFP-10 and Rv3615c formulated as a ‘ready to use’ reagent by a commercial manufacturer. Our results demonstrate that, unlike tuberculin reagents, a diagnostic skin test using DST-F maintained high specificity in BCG vaccinated animals. Furthermore, the DST-F skin test demonstrated a high relative sensitivity in identifying M. bovis infected animals, including those where BCG vaccination failed to prevent bovine TB pathology following experimental exposure to M. bovis. The DST-F is currently undergoing field trials in Great Britain to support its licensure and commercialisation. Nature Publishing Group UK 2022-07-14 /pmc/articles/PMC9283413/ /pubmed/35835806 http://dx.doi.org/10.1038/s41598-022-16092-8 Text en © Crown 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Jones, Gareth J. Konold, Timm Hurley, Shellene Holder, Tom Steinbach, Sabine Coad, Mick Neil Wedlock, D. Buddle, Bryce M. Singh, Mahavir Martin Vordermeier, H. Test performance data demonstrates utility of a cattle DIVA skin test reagent (DST-F) compatible with BCG vaccination |
title | Test performance data demonstrates utility of a cattle DIVA skin test reagent (DST-F) compatible with BCG vaccination |
title_full | Test performance data demonstrates utility of a cattle DIVA skin test reagent (DST-F) compatible with BCG vaccination |
title_fullStr | Test performance data demonstrates utility of a cattle DIVA skin test reagent (DST-F) compatible with BCG vaccination |
title_full_unstemmed | Test performance data demonstrates utility of a cattle DIVA skin test reagent (DST-F) compatible with BCG vaccination |
title_short | Test performance data demonstrates utility of a cattle DIVA skin test reagent (DST-F) compatible with BCG vaccination |
title_sort | test performance data demonstrates utility of a cattle diva skin test reagent (dst-f) compatible with bcg vaccination |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283413/ https://www.ncbi.nlm.nih.gov/pubmed/35835806 http://dx.doi.org/10.1038/s41598-022-16092-8 |
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