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BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC

Purpose: Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors of the head and neck, while metastasis is the main cause of OSCC-related death. There is an urgent need to explore novel prognostic biomarkers and identify biological targets related to metastasis in OSCC treatme...

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Autores principales: Shen, Ting, Yang, Tianru, Yao, Mianfeng, Zheng, Ziran, He, Mi, Shao, Mengying, Li, Jiang, Fang, Changyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283838/
https://www.ncbi.nlm.nih.gov/pubmed/35846125
http://dx.doi.org/10.3389/fgene.2022.875617
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author Shen, Ting
Yang, Tianru
Yao, Mianfeng
Zheng, Ziran
He, Mi
Shao, Mengying
Li, Jiang
Fang, Changyun
author_facet Shen, Ting
Yang, Tianru
Yao, Mianfeng
Zheng, Ziran
He, Mi
Shao, Mengying
Li, Jiang
Fang, Changyun
author_sort Shen, Ting
collection PubMed
description Purpose: Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors of the head and neck, while metastasis is the main cause of OSCC-related death. There is an urgent need to explore novel prognostic biomarkers and identify biological targets related to metastasis in OSCC treatment. Methods: Analysis of differential expression was performed using datasets in The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). Immunohistochemistry (IHC) was conducted to assess the expression of betacellulin (BTC) in OSCC. SCC4 and CAL27 cells were used for in vitro experiments, in which CCK-8, transwell assays, and wounding healing assays were performed to verify the biological functions of BTC. The role of BTC in EMT was analyzed by EMT score and Western blot. Results: Through the analysis of the mRNA expression profile data from TCGA database in OSCC, we found that only low expression of BTC was significantly correlated with a poor prognosis in OSCC patients. The results of IHC assays and TCGA databases showed that the expression level of BTC was related to the tumor stage, histological grade, and metastasis status. In vitro analysis showed that overexpression of BTC significantly suppressed the proliferation and migration of OSCC cells. Furthermore, we confirmed that BTC could affect EMT through the PI3K-AKT signaling pathway. Conclusion: The overexpression of BTC suppresses the proliferation, migration, and EMT of OSCC cells via the PI3K-AKT pathways, leading to a better prognosis in OSCC. BTC may be used as a novel molecular marker to assess the prognosis of OSCC patients.
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spelling pubmed-92838382022-07-16 BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC Shen, Ting Yang, Tianru Yao, Mianfeng Zheng, Ziran He, Mi Shao, Mengying Li, Jiang Fang, Changyun Front Genet Genetics Purpose: Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors of the head and neck, while metastasis is the main cause of OSCC-related death. There is an urgent need to explore novel prognostic biomarkers and identify biological targets related to metastasis in OSCC treatment. Methods: Analysis of differential expression was performed using datasets in The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). Immunohistochemistry (IHC) was conducted to assess the expression of betacellulin (BTC) in OSCC. SCC4 and CAL27 cells were used for in vitro experiments, in which CCK-8, transwell assays, and wounding healing assays were performed to verify the biological functions of BTC. The role of BTC in EMT was analyzed by EMT score and Western blot. Results: Through the analysis of the mRNA expression profile data from TCGA database in OSCC, we found that only low expression of BTC was significantly correlated with a poor prognosis in OSCC patients. The results of IHC assays and TCGA databases showed that the expression level of BTC was related to the tumor stage, histological grade, and metastasis status. In vitro analysis showed that overexpression of BTC significantly suppressed the proliferation and migration of OSCC cells. Furthermore, we confirmed that BTC could affect EMT through the PI3K-AKT signaling pathway. Conclusion: The overexpression of BTC suppresses the proliferation, migration, and EMT of OSCC cells via the PI3K-AKT pathways, leading to a better prognosis in OSCC. BTC may be used as a novel molecular marker to assess the prognosis of OSCC patients. Frontiers Media S.A. 2022-07-01 /pmc/articles/PMC9283838/ /pubmed/35846125 http://dx.doi.org/10.3389/fgene.2022.875617 Text en Copyright © 2022 Shen, Yang, Yao, Zheng, He, Shao, Li and Fang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Shen, Ting
Yang, Tianru
Yao, Mianfeng
Zheng, Ziran
He, Mi
Shao, Mengying
Li, Jiang
Fang, Changyun
BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC
title BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC
title_full BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC
title_fullStr BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC
title_full_unstemmed BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC
title_short BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC
title_sort btc as a novel biomarker contributing to emt via the pi3k-akt pathway in oscc
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283838/
https://www.ncbi.nlm.nih.gov/pubmed/35846125
http://dx.doi.org/10.3389/fgene.2022.875617
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