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Human whole mitochondrial genome sequencing and analysis: optimization of the experimental workflow

AIM: To evaluate critical steps in Illumina® Human mtDNA Genome assay: target enrichment, limited-cycle PCR, and library normalization, in order to optimize the protocol for analysis of whole mitochondrial genomes from human reference samples. METHODS: Three long-range high-fidelity DNA polymerases...

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Detalles Bibliográficos
Autores principales: Sukser, Viktorija, Korolija, Marina, Račić, Ivana, Rožić, Sara, Barbarić, Lucija
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Croatian Medical Schools 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284014/
https://www.ncbi.nlm.nih.gov/pubmed/35722691
http://dx.doi.org/10.3325/cmj.2022.63.224