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Integrated Analytical System for Clinical Single‐Cell Analysis

High‐dimensional analyses of cancers can potentially be used to better define cancer subtypes, analyze the complex tumor microenvironment, and perform cancer cell pathway analyses for drug trials. Unfortunately, integrated systems that allow such analyses in serial fine needle aspirates within a day...

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Detalles Bibliográficos
Autores principales: Peterson, Hannah M., Chin, Lip Ket, Iwamoto, Yoshi, Oh, Juhyun, Carlson, Jonathan C. T., Lee, Hakho, Im, Hyungsoon, Weissleder, Ralph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284190/
https://www.ncbi.nlm.nih.gov/pubmed/35508767
http://dx.doi.org/10.1002/advs.202200415
Descripción
Sumario:High‐dimensional analyses of cancers can potentially be used to better define cancer subtypes, analyze the complex tumor microenvironment, and perform cancer cell pathway analyses for drug trials. Unfortunately, integrated systems that allow such analyses in serial fine needle aspirates within a day or at point‐of‐care currently do not exist. To achieve this, an integrated immunofluorescence single‐cell analyzer (i2SCAN) for deep profiling of directly harvested cells is developed. By combining a novel cellular imaging system, highly cyclable bioorthogonal FAST antibody panels, and integrated computational analysis, it is shown that same‐day analysis is possible in thousands of harvested cells. It is demonstrated that the i2SCAN approach allows comprehensive analysis of breast cancer samples obtained by fine needle aspiration or core tissues. The method is a rapid, robust, and low‐cost solution to high‐dimensional analysis of scant clinical specimens.