Cargando…

AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers

Floral terpenoid volatiles are impacted by light quality. In snapdragon, blue light can significantly enhance the emissions of ocimene and myrcene and the expression of ocimene synthase (AmOCS) and myrcene synthase (AmMYS). However, the mechanisms underlying the response to blue light are largely un...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Jianing, Li, Tong, Wang, Xuelian, Zhang, Xi, Bai, Xiaoning, Shao, Huihui, Wang, Shaojie, Hu, Zenghui, Wu, Jing, Leng, Pingsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284265/
https://www.ncbi.nlm.nih.gov/pubmed/35845643
http://dx.doi.org/10.3389/fpls.2022.885168
_version_ 1784747525233704960
author Han, Jianing
Li, Tong
Wang, Xuelian
Zhang, Xi
Bai, Xiaoning
Shao, Huihui
Wang, Shaojie
Hu, Zenghui
Wu, Jing
Leng, Pingsheng
author_facet Han, Jianing
Li, Tong
Wang, Xuelian
Zhang, Xi
Bai, Xiaoning
Shao, Huihui
Wang, Shaojie
Hu, Zenghui
Wu, Jing
Leng, Pingsheng
author_sort Han, Jianing
collection PubMed
description Floral terpenoid volatiles are impacted by light quality. In snapdragon, blue light can significantly enhance the emissions of ocimene and myrcene and the expression of ocimene synthase (AmOCS) and myrcene synthase (AmMYS). However, the mechanisms underlying the response to blue light are largely unknown. In this study, two transcription factors (TFs), AmMYB24 and AmMYB63 were screened which showed high expression level under blue light. AmMYB24 exhibited synchronous expression with AmOCS. Moreover, AmOCS transcript expression was up-regulated in response to AmMYB24 overexpression. This activation is direct and occurs through binding of AmMYB24 to MYBCORECYCATB1 sites in the AmOCS promoter. In addition, AmMYB24 interacts with the blue light signal key receptor AmCRY1 and the transcriptional activation activity of AmMYB24 was decreased in AmCRY1 silencing flowers. Taken together, our results revealed the regulatory pathway of biosynthesis of ocimene induced by blue light mediated by AmMYB24 and AmCRY1. When snapdragon flowers were exposed to blue light, AmCRY1 was first activated, the light signal is transduced to AmMYB24 through interaction with AmCRY1, and finally AmMYB24 activates AmOCS by binding to its MYBCOREATCYCB1 motif, resulting in abundant ocimene emission.
format Online
Article
Text
id pubmed-9284265
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92842652022-07-16 AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers Han, Jianing Li, Tong Wang, Xuelian Zhang, Xi Bai, Xiaoning Shao, Huihui Wang, Shaojie Hu, Zenghui Wu, Jing Leng, Pingsheng Front Plant Sci Plant Science Floral terpenoid volatiles are impacted by light quality. In snapdragon, blue light can significantly enhance the emissions of ocimene and myrcene and the expression of ocimene synthase (AmOCS) and myrcene synthase (AmMYS). However, the mechanisms underlying the response to blue light are largely unknown. In this study, two transcription factors (TFs), AmMYB24 and AmMYB63 were screened which showed high expression level under blue light. AmMYB24 exhibited synchronous expression with AmOCS. Moreover, AmOCS transcript expression was up-regulated in response to AmMYB24 overexpression. This activation is direct and occurs through binding of AmMYB24 to MYBCORECYCATB1 sites in the AmOCS promoter. In addition, AmMYB24 interacts with the blue light signal key receptor AmCRY1 and the transcriptional activation activity of AmMYB24 was decreased in AmCRY1 silencing flowers. Taken together, our results revealed the regulatory pathway of biosynthesis of ocimene induced by blue light mediated by AmMYB24 and AmCRY1. When snapdragon flowers were exposed to blue light, AmCRY1 was first activated, the light signal is transduced to AmMYB24 through interaction with AmCRY1, and finally AmMYB24 activates AmOCS by binding to its MYBCOREATCYCB1 motif, resulting in abundant ocimene emission. Frontiers Media S.A. 2022-07-01 /pmc/articles/PMC9284265/ /pubmed/35845643 http://dx.doi.org/10.3389/fpls.2022.885168 Text en Copyright © 2022 Han, Li, Wang, Zhang, Bai, Shao, Wang, Hu, Wu and Leng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Han, Jianing
Li, Tong
Wang, Xuelian
Zhang, Xi
Bai, Xiaoning
Shao, Huihui
Wang, Shaojie
Hu, Zenghui
Wu, Jing
Leng, Pingsheng
AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers
title AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers
title_full AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers
title_fullStr AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers
title_full_unstemmed AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers
title_short AmMYB24 Regulates Floral Terpenoid Biosynthesis Induced by Blue Light in Snapdragon Flowers
title_sort ammyb24 regulates floral terpenoid biosynthesis induced by blue light in snapdragon flowers
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284265/
https://www.ncbi.nlm.nih.gov/pubmed/35845643
http://dx.doi.org/10.3389/fpls.2022.885168
work_keys_str_mv AT hanjianing ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers
AT litong ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers
AT wangxuelian ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers
AT zhangxi ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers
AT baixiaoning ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers
AT shaohuihui ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers
AT wangshaojie ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers
AT huzenghui ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers
AT wujing ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers
AT lengpingsheng ammyb24regulatesfloralterpenoidbiosynthesisinducedbybluelightinsnapdragonflowers