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Protocol to detect smooth muscle actin-alpha and measure oxidative damage in neonatal mouse intestine

This protocol describes how to characterize α-Smooth muscle actin (αSMA) spatiotemporal expression during mouse small intestinal development. Specific tissue fixation preserves αSMA arrangement in low αSMA expressing cells that are conventionally undetectable under αSMA immunofluorescent stain due t...

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Detalles Bibliográficos
Autores principales:	Hu, Shing, Sevier, Carolyn S., Kurpios, Natasza A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284457/ https://www.ncbi.nlm.nih.gov/pubmed/35810413 http://dx.doi.org/10.1016/j.xpro.2022.101524

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