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Three-Dimensional Visualization of Mouse Endometrial Remodeling After Superovulation

Uterine status determines pregnancy success. Although it is well known that superovulation operations can disrupt uterine function, our understanding of the morphological changes in the uterine endometrium at the three-dimensional (3D) level is limited. Here, combining the tissue clearing with 3D de...

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Autores principales: Qu, Yongcun, Zhang, Jia, Guo, Shanshan, Zhang, Liwen, Qian, Jingjing, Zhu, Xili, Duan, Enkui, Zhang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284589/
https://www.ncbi.nlm.nih.gov/pubmed/35846371
http://dx.doi.org/10.3389/fcell.2022.933852
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author Qu, Yongcun
Zhang, Jia
Guo, Shanshan
Zhang, Liwen
Qian, Jingjing
Zhu, Xili
Duan, Enkui
Zhang, Ying
author_facet Qu, Yongcun
Zhang, Jia
Guo, Shanshan
Zhang, Liwen
Qian, Jingjing
Zhu, Xili
Duan, Enkui
Zhang, Ying
author_sort Qu, Yongcun
collection PubMed
description Uterine status determines pregnancy success. Although it is well known that superovulation operations can disrupt uterine function, our understanding of the morphological changes in the uterine endometrium at the three-dimensional (3D) level is limited. Here, combining the tissue clearing with 3D deep imaging, we reveal an increase in epithelial density and angiogenesis after ovarian stimulation, which is accompanied by a circulating surge in P4 levels. Using an ovariectomized mouse model, we further detected the separate regulatory effects of P4 and E2 on the uterine endometrium, with P4 promoting endothelial cell growth and E2 inducing epithelial proliferation. Additionally, we observed that the effects of E2 can be partially neutralized by P4, and vice versa. By analyzing the 3D uterine imaging, we discovered an interesting phenomenon in which the growing blood vessels closely surround the remodeling uterine epithelium, indicating a close relationship between angiogenesis and epithelial growth. These findings provide new insight into the uterine epithelial changes and angiogenesis at the 3D level, and explain a potential reason for endometrial changes due to the low implantation rate in patients undergoing clinic super-ovulation.
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spelling pubmed-92845892022-07-16 Three-Dimensional Visualization of Mouse Endometrial Remodeling After Superovulation Qu, Yongcun Zhang, Jia Guo, Shanshan Zhang, Liwen Qian, Jingjing Zhu, Xili Duan, Enkui Zhang, Ying Front Cell Dev Biol Cell and Developmental Biology Uterine status determines pregnancy success. Although it is well known that superovulation operations can disrupt uterine function, our understanding of the morphological changes in the uterine endometrium at the three-dimensional (3D) level is limited. Here, combining the tissue clearing with 3D deep imaging, we reveal an increase in epithelial density and angiogenesis after ovarian stimulation, which is accompanied by a circulating surge in P4 levels. Using an ovariectomized mouse model, we further detected the separate regulatory effects of P4 and E2 on the uterine endometrium, with P4 promoting endothelial cell growth and E2 inducing epithelial proliferation. Additionally, we observed that the effects of E2 can be partially neutralized by P4, and vice versa. By analyzing the 3D uterine imaging, we discovered an interesting phenomenon in which the growing blood vessels closely surround the remodeling uterine epithelium, indicating a close relationship between angiogenesis and epithelial growth. These findings provide new insight into the uterine epithelial changes and angiogenesis at the 3D level, and explain a potential reason for endometrial changes due to the low implantation rate in patients undergoing clinic super-ovulation. Frontiers Media S.A. 2022-06-29 /pmc/articles/PMC9284589/ /pubmed/35846371 http://dx.doi.org/10.3389/fcell.2022.933852 Text en Copyright © 2022 Qu, Zhang, Guo, Zhang, Qian, Zhu, Duan and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Qu, Yongcun
Zhang, Jia
Guo, Shanshan
Zhang, Liwen
Qian, Jingjing
Zhu, Xili
Duan, Enkui
Zhang, Ying
Three-Dimensional Visualization of Mouse Endometrial Remodeling After Superovulation
title Three-Dimensional Visualization of Mouse Endometrial Remodeling After Superovulation
title_full Three-Dimensional Visualization of Mouse Endometrial Remodeling After Superovulation
title_fullStr Three-Dimensional Visualization of Mouse Endometrial Remodeling After Superovulation
title_full_unstemmed Three-Dimensional Visualization of Mouse Endometrial Remodeling After Superovulation
title_short Three-Dimensional Visualization of Mouse Endometrial Remodeling After Superovulation
title_sort three-dimensional visualization of mouse endometrial remodeling after superovulation
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284589/
https://www.ncbi.nlm.nih.gov/pubmed/35846371
http://dx.doi.org/10.3389/fcell.2022.933852
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