Cargando…

Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation

BACKGROUND: Intestinal inflammation is prevalent in chicken, which results in decreased growth performance and considerable economic losses. Accumulated findings established the close relationship between gut microbiota and chicken growth performance. However, whether gut microbiota impacts chicken...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Xiaolong, Akhtar, Muhammad, Chen, Yan, Ma, Ziyu, Liang, Yuyun, Shi, Deshi, Cheng, Ranran, Cui, Lei, Hu, Yafang, Nafady, Abdallah A., Ansari, Abdur Rahman, Abdel-Kafy, El-Sayed M., Liu, Huazhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284917/
https://www.ncbi.nlm.nih.gov/pubmed/35836252
http://dx.doi.org/10.1186/s40168-022-01299-8
_version_ 1784747668398931968
author Zhang, Xiaolong
Akhtar, Muhammad
Chen, Yan
Ma, Ziyu
Liang, Yuyun
Shi, Deshi
Cheng, Ranran
Cui, Lei
Hu, Yafang
Nafady, Abdallah A.
Ansari, Abdur Rahman
Abdel-Kafy, El-Sayed M.
Liu, Huazhen
author_facet Zhang, Xiaolong
Akhtar, Muhammad
Chen, Yan
Ma, Ziyu
Liang, Yuyun
Shi, Deshi
Cheng, Ranran
Cui, Lei
Hu, Yafang
Nafady, Abdallah A.
Ansari, Abdur Rahman
Abdel-Kafy, El-Sayed M.
Liu, Huazhen
author_sort Zhang, Xiaolong
collection PubMed
description BACKGROUND: Intestinal inflammation is prevalent in chicken, which results in decreased growth performance and considerable economic losses. Accumulated findings established the close relationship between gut microbiota and chicken growth performance. However, whether gut microbiota impacts chicken growth performance by lessening intestinal inflammation remains elusive. RESULTS: Seven-weeks-old male and female chickens with the highest or lowest body weights were significantly different in breast and leg muscle indices and average cross-sectional area of muscle cells. 16S rRNA gene sequencing indicated Gram-positive bacteria, such as Lactobacilli, were the predominant species in high body weight chickens. Conversely, Gram-negative bacteria, such as Comamonas, Acinetobacter, Brucella, Escherichia-Shigella, Thermus, Undibacterium, and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium were significantly abundant in low body weight chickens. Serum lipopolysaccharide (LPS) level was significantly higher in low body weight chickens (101.58 ± 5.78 ng/mL) compared with high body weight chickens (85.12 ± 4.79 ng/mL). The expression of TLR4, NF-κB, MyD88, and related inflammatory cytokines in the jejunum was significantly upregulated in low body weight chickens, which led to the damage of gut barrier integrity. Furthermore, transferring fecal microbiota from adult chickens with high body weight into 1-day-old chicks reshaped the jejunal microbiota, mitigated inflammatory response, and improved chicken growth performance. CONCLUSIONS: Our findings suggested that jejunal microbiota could affect chicken growth performance by mitigating intestinal inflammation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-022-01299-8.
format Online
Article
Text
id pubmed-9284917
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-92849172022-07-16 Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation Zhang, Xiaolong Akhtar, Muhammad Chen, Yan Ma, Ziyu Liang, Yuyun Shi, Deshi Cheng, Ranran Cui, Lei Hu, Yafang Nafady, Abdallah A. Ansari, Abdur Rahman Abdel-Kafy, El-Sayed M. Liu, Huazhen Microbiome Research BACKGROUND: Intestinal inflammation is prevalent in chicken, which results in decreased growth performance and considerable economic losses. Accumulated findings established the close relationship between gut microbiota and chicken growth performance. However, whether gut microbiota impacts chicken growth performance by lessening intestinal inflammation remains elusive. RESULTS: Seven-weeks-old male and female chickens with the highest or lowest body weights were significantly different in breast and leg muscle indices and average cross-sectional area of muscle cells. 16S rRNA gene sequencing indicated Gram-positive bacteria, such as Lactobacilli, were the predominant species in high body weight chickens. Conversely, Gram-negative bacteria, such as Comamonas, Acinetobacter, Brucella, Escherichia-Shigella, Thermus, Undibacterium, and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium were significantly abundant in low body weight chickens. Serum lipopolysaccharide (LPS) level was significantly higher in low body weight chickens (101.58 ± 5.78 ng/mL) compared with high body weight chickens (85.12 ± 4.79 ng/mL). The expression of TLR4, NF-κB, MyD88, and related inflammatory cytokines in the jejunum was significantly upregulated in low body weight chickens, which led to the damage of gut barrier integrity. Furthermore, transferring fecal microbiota from adult chickens with high body weight into 1-day-old chicks reshaped the jejunal microbiota, mitigated inflammatory response, and improved chicken growth performance. CONCLUSIONS: Our findings suggested that jejunal microbiota could affect chicken growth performance by mitigating intestinal inflammation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-022-01299-8. BioMed Central 2022-07-15 /pmc/articles/PMC9284917/ /pubmed/35836252 http://dx.doi.org/10.1186/s40168-022-01299-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Xiaolong
Akhtar, Muhammad
Chen, Yan
Ma, Ziyu
Liang, Yuyun
Shi, Deshi
Cheng, Ranran
Cui, Lei
Hu, Yafang
Nafady, Abdallah A.
Ansari, Abdur Rahman
Abdel-Kafy, El-Sayed M.
Liu, Huazhen
Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation
title Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation
title_full Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation
title_fullStr Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation
title_full_unstemmed Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation
title_short Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation
title_sort chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284917/
https://www.ncbi.nlm.nih.gov/pubmed/35836252
http://dx.doi.org/10.1186/s40168-022-01299-8
work_keys_str_mv AT zhangxiaolong chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT akhtarmuhammad chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT chenyan chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT maziyu chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT liangyuyun chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT shideshi chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT chengranran chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT cuilei chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT huyafang chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT nafadyabdallaha chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT ansariabdurrahman chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT abdelkafyelsayedm chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation
AT liuhuazhen chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation