Cargando…
Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation
BACKGROUND: Intestinal inflammation is prevalent in chicken, which results in decreased growth performance and considerable economic losses. Accumulated findings established the close relationship between gut microbiota and chicken growth performance. However, whether gut microbiota impacts chicken...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284917/ https://www.ncbi.nlm.nih.gov/pubmed/35836252 http://dx.doi.org/10.1186/s40168-022-01299-8 |
_version_ | 1784747668398931968 |
---|---|
author | Zhang, Xiaolong Akhtar, Muhammad Chen, Yan Ma, Ziyu Liang, Yuyun Shi, Deshi Cheng, Ranran Cui, Lei Hu, Yafang Nafady, Abdallah A. Ansari, Abdur Rahman Abdel-Kafy, El-Sayed M. Liu, Huazhen |
author_facet | Zhang, Xiaolong Akhtar, Muhammad Chen, Yan Ma, Ziyu Liang, Yuyun Shi, Deshi Cheng, Ranran Cui, Lei Hu, Yafang Nafady, Abdallah A. Ansari, Abdur Rahman Abdel-Kafy, El-Sayed M. Liu, Huazhen |
author_sort | Zhang, Xiaolong |
collection | PubMed |
description | BACKGROUND: Intestinal inflammation is prevalent in chicken, which results in decreased growth performance and considerable economic losses. Accumulated findings established the close relationship between gut microbiota and chicken growth performance. However, whether gut microbiota impacts chicken growth performance by lessening intestinal inflammation remains elusive. RESULTS: Seven-weeks-old male and female chickens with the highest or lowest body weights were significantly different in breast and leg muscle indices and average cross-sectional area of muscle cells. 16S rRNA gene sequencing indicated Gram-positive bacteria, such as Lactobacilli, were the predominant species in high body weight chickens. Conversely, Gram-negative bacteria, such as Comamonas, Acinetobacter, Brucella, Escherichia-Shigella, Thermus, Undibacterium, and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium were significantly abundant in low body weight chickens. Serum lipopolysaccharide (LPS) level was significantly higher in low body weight chickens (101.58 ± 5.78 ng/mL) compared with high body weight chickens (85.12 ± 4.79 ng/mL). The expression of TLR4, NF-κB, MyD88, and related inflammatory cytokines in the jejunum was significantly upregulated in low body weight chickens, which led to the damage of gut barrier integrity. Furthermore, transferring fecal microbiota from adult chickens with high body weight into 1-day-old chicks reshaped the jejunal microbiota, mitigated inflammatory response, and improved chicken growth performance. CONCLUSIONS: Our findings suggested that jejunal microbiota could affect chicken growth performance by mitigating intestinal inflammation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-022-01299-8. |
format | Online Article Text |
id | pubmed-9284917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92849172022-07-16 Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation Zhang, Xiaolong Akhtar, Muhammad Chen, Yan Ma, Ziyu Liang, Yuyun Shi, Deshi Cheng, Ranran Cui, Lei Hu, Yafang Nafady, Abdallah A. Ansari, Abdur Rahman Abdel-Kafy, El-Sayed M. Liu, Huazhen Microbiome Research BACKGROUND: Intestinal inflammation is prevalent in chicken, which results in decreased growth performance and considerable economic losses. Accumulated findings established the close relationship between gut microbiota and chicken growth performance. However, whether gut microbiota impacts chicken growth performance by lessening intestinal inflammation remains elusive. RESULTS: Seven-weeks-old male and female chickens with the highest or lowest body weights were significantly different in breast and leg muscle indices and average cross-sectional area of muscle cells. 16S rRNA gene sequencing indicated Gram-positive bacteria, such as Lactobacilli, were the predominant species in high body weight chickens. Conversely, Gram-negative bacteria, such as Comamonas, Acinetobacter, Brucella, Escherichia-Shigella, Thermus, Undibacterium, and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium were significantly abundant in low body weight chickens. Serum lipopolysaccharide (LPS) level was significantly higher in low body weight chickens (101.58 ± 5.78 ng/mL) compared with high body weight chickens (85.12 ± 4.79 ng/mL). The expression of TLR4, NF-κB, MyD88, and related inflammatory cytokines in the jejunum was significantly upregulated in low body weight chickens, which led to the damage of gut barrier integrity. Furthermore, transferring fecal microbiota from adult chickens with high body weight into 1-day-old chicks reshaped the jejunal microbiota, mitigated inflammatory response, and improved chicken growth performance. CONCLUSIONS: Our findings suggested that jejunal microbiota could affect chicken growth performance by mitigating intestinal inflammation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40168-022-01299-8. BioMed Central 2022-07-15 /pmc/articles/PMC9284917/ /pubmed/35836252 http://dx.doi.org/10.1186/s40168-022-01299-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhang, Xiaolong Akhtar, Muhammad Chen, Yan Ma, Ziyu Liang, Yuyun Shi, Deshi Cheng, Ranran Cui, Lei Hu, Yafang Nafady, Abdallah A. Ansari, Abdur Rahman Abdel-Kafy, El-Sayed M. Liu, Huazhen Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation |
title | Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation |
title_full | Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation |
title_fullStr | Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation |
title_full_unstemmed | Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation |
title_short | Chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation |
title_sort | chicken jejunal microbiota improves growth performance by mitigating intestinal inflammation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284917/ https://www.ncbi.nlm.nih.gov/pubmed/35836252 http://dx.doi.org/10.1186/s40168-022-01299-8 |
work_keys_str_mv | AT zhangxiaolong chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT akhtarmuhammad chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT chenyan chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT maziyu chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT liangyuyun chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT shideshi chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT chengranran chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT cuilei chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT huyafang chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT nafadyabdallaha chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT ansariabdurrahman chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT abdelkafyelsayedm chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation AT liuhuazhen chickenjejunalmicrobiotaimprovesgrowthperformancebymitigatingintestinalinflammation |