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Microplitis bicoloratus bracovirus regulates cyclophilin A‐apoptosis‐inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process
Microplitis bicoloratus bracovirus (MbBV) induces apoptosis in hemocytes of the host (Spodoptera litura) via the cyclophilin A (CypA)‐mediated signaling pathway. However, the mechanisms underlying CypA‐mediated signaling during apoptosis remain largely unknown. Therefore, in this study, we investiga...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285338/ https://www.ncbi.nlm.nih.gov/pubmed/35218062 http://dx.doi.org/10.1002/arch.21877 |
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author | Hu, Yan Liang, Ya‐Ping Tian, Hang‐Yu Xu, Cui‐Xian Yu, Dan Zhang, Pan Ye, Hui Li, Ming |
author_facet | Hu, Yan Liang, Ya‐Ping Tian, Hang‐Yu Xu, Cui‐Xian Yu, Dan Zhang, Pan Ye, Hui Li, Ming |
author_sort | Hu, Yan |
collection | PubMed |
description | Microplitis bicoloratus bracovirus (MbBV) induces apoptosis in hemocytes of the host (Spodoptera litura) via the cyclophilin A (CypA)‐mediated signaling pathway. However, the mechanisms underlying CypA‐mediated signaling during apoptosis remain largely unknown. Therefore, in this study, we investigated how CypA and apoptosis‐inducing factor (AIF) interact during MbBV‐mediated apoptosis. Our findings showed that MbBV induces apoptosis through the CypA‐AIF axis of insect immune suppression. In MbBV‐infected Spli221 cells, both the expression of the cypa gene and the release of AIF from the mitochondria increased the number of apoptotic cells. CypA and AIF underwent concurrent cytoplasm‐nuclear translocation. Conversely, blocking of AIF release from mitochondria not only inhibited the CypA‐AIF interaction but also inhibited the cytoplasmic‐nuclear translocation of AIF and CypA. Importantly, the survival of the apoptotic phenotype was significantly rescued in MbBV‐infected Spli221 cells. In addition, we found that the cyclosporine A‐mediated inhibition of CypA did not prevent the formation of the CypA and AIF complex; rather, this only suppressed genomic DNA fragmentation. In vitro experiments revealed direct molecular interactions between recombinant CypA and AIF. Taken together, our results demonstrate that the CypA‐AIF interaction plays an important role in MbBV‐induced innate immune suppression. This study will help to clarify aspects of insect immunological mechanisms and will be relevant to biological pest control. |
format | Online Article Text |
id | pubmed-9285338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92853382022-07-15 Microplitis bicoloratus bracovirus regulates cyclophilin A‐apoptosis‐inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process Hu, Yan Liang, Ya‐Ping Tian, Hang‐Yu Xu, Cui‐Xian Yu, Dan Zhang, Pan Ye, Hui Li, Ming Arch Insect Biochem Physiol Research Articles Microplitis bicoloratus bracovirus (MbBV) induces apoptosis in hemocytes of the host (Spodoptera litura) via the cyclophilin A (CypA)‐mediated signaling pathway. However, the mechanisms underlying CypA‐mediated signaling during apoptosis remain largely unknown. Therefore, in this study, we investigated how CypA and apoptosis‐inducing factor (AIF) interact during MbBV‐mediated apoptosis. Our findings showed that MbBV induces apoptosis through the CypA‐AIF axis of insect immune suppression. In MbBV‐infected Spli221 cells, both the expression of the cypa gene and the release of AIF from the mitochondria increased the number of apoptotic cells. CypA and AIF underwent concurrent cytoplasm‐nuclear translocation. Conversely, blocking of AIF release from mitochondria not only inhibited the CypA‐AIF interaction but also inhibited the cytoplasmic‐nuclear translocation of AIF and CypA. Importantly, the survival of the apoptotic phenotype was significantly rescued in MbBV‐infected Spli221 cells. In addition, we found that the cyclosporine A‐mediated inhibition of CypA did not prevent the formation of the CypA and AIF complex; rather, this only suppressed genomic DNA fragmentation. In vitro experiments revealed direct molecular interactions between recombinant CypA and AIF. Taken together, our results demonstrate that the CypA‐AIF interaction plays an important role in MbBV‐induced innate immune suppression. This study will help to clarify aspects of insect immunological mechanisms and will be relevant to biological pest control. John Wiley and Sons Inc. 2022-02-25 2022-05 /pmc/articles/PMC9285338/ /pubmed/35218062 http://dx.doi.org/10.1002/arch.21877 Text en © 2022 The Authors. Archives of Insect Biochemistry and Physiology Published by Wiley Periodicals LLC https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Hu, Yan Liang, Ya‐Ping Tian, Hang‐Yu Xu, Cui‐Xian Yu, Dan Zhang, Pan Ye, Hui Li, Ming Microplitis bicoloratus bracovirus regulates cyclophilin A‐apoptosis‐inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process |
title |
Microplitis bicoloratus bracovirus regulates cyclophilin A‐apoptosis‐inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process |
title_full |
Microplitis bicoloratus bracovirus regulates cyclophilin A‐apoptosis‐inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process |
title_fullStr |
Microplitis bicoloratus bracovirus regulates cyclophilin A‐apoptosis‐inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process |
title_full_unstemmed |
Microplitis bicoloratus bracovirus regulates cyclophilin A‐apoptosis‐inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process |
title_short |
Microplitis bicoloratus bracovirus regulates cyclophilin A‐apoptosis‐inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process |
title_sort | microplitis bicoloratus bracovirus regulates cyclophilin a‐apoptosis‐inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285338/ https://www.ncbi.nlm.nih.gov/pubmed/35218062 http://dx.doi.org/10.1002/arch.21877 |
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