Cargando…
Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil—a retrospective study
BACKGROUND: A high prevalence and incidence of head and neck tumors make Brazil the country with the third-highest number of cases of these malignant neoplasms. The main risk factors are smoking and alcohol consumption; however, cases related to the human papillomavirus (HPV) have tripled in number,...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285469/ https://www.ncbi.nlm.nih.gov/pubmed/35846883 http://dx.doi.org/10.7717/peerj.13684 |
_version_ | 1784747788320374784 |
---|---|
author | Brito, Charlles Cossetti, Rachel D. de Souza, Diego Agra Catanha, Marcos de Matos Monteiro, Pablo Vidal, Flavia Castello Branco |
author_facet | Brito, Charlles Cossetti, Rachel D. de Souza, Diego Agra Catanha, Marcos de Matos Monteiro, Pablo Vidal, Flavia Castello Branco |
author_sort | Brito, Charlles |
collection | PubMed |
description | BACKGROUND: A high prevalence and incidence of head and neck tumors make Brazil the country with the third-highest number of cases of these malignant neoplasms. The main risk factors are smoking and alcohol consumption; however, cases related to the human papillomavirus (HPV) have tripled in number, demonstrating a changing disease profile. Studies have reported the prevalence of HPV in laryngeal squamous cell carcinoma (LSCC) to vary between 8% and 83%. The role of HPV as an important causative factor in LSCC remains unclear. METHODS: This retrospective study included 82 patients with LSCC diagnosed between 2014 and 2019 at two oncology hospitals in São Luís, Brazil. Sociodemographic and clinical data, and the histopathologic characteristics of the tumors, were collected directly from medical records. Genetic material was extracted from paraffin-embedded samples using nested polymerase chain reaction (PCR) and automated sequencing for HPV detection and genotyping. The results by social and clinicopathologic variables were then compared using the chi-squared test and multivariate analysis. RESULTS: Sociodemographic analysesdemonstrated that most patients were men (87.8%), brown-skinned (75.6%), and resident in the state capital (53.7%). They generally had a poor education status (53.7%), having only an elementary school education (completed/incomplete), and 51.2% were self-employed in occupations such as farming or fishing. Smoking and alcohol consumption habits were observed in approximately half the patients. With respect to clinical characteristics, 39% of patients exhibited T1/T2 staging, 51.2% had no distant metastasis, and 30.5% had lymph node invasion. HPV DNA was detected in half the samples (50%), with the high oncogenic type 16 being the most prevalent. There was no significant relationship observed between the economic, educational, occupational with the HPV LSCC in the presented data, although multivariate analysis demonstrated that HPV DNA was more likely to be present in T3–T4 tumors (p = 0.002). |
format | Online Article Text |
id | pubmed-9285469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92854692022-07-16 Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil—a retrospective study Brito, Charlles Cossetti, Rachel D. de Souza, Diego Agra Catanha, Marcos de Matos Monteiro, Pablo Vidal, Flavia Castello Branco PeerJ Molecular Biology BACKGROUND: A high prevalence and incidence of head and neck tumors make Brazil the country with the third-highest number of cases of these malignant neoplasms. The main risk factors are smoking and alcohol consumption; however, cases related to the human papillomavirus (HPV) have tripled in number, demonstrating a changing disease profile. Studies have reported the prevalence of HPV in laryngeal squamous cell carcinoma (LSCC) to vary between 8% and 83%. The role of HPV as an important causative factor in LSCC remains unclear. METHODS: This retrospective study included 82 patients with LSCC diagnosed between 2014 and 2019 at two oncology hospitals in São Luís, Brazil. Sociodemographic and clinical data, and the histopathologic characteristics of the tumors, were collected directly from medical records. Genetic material was extracted from paraffin-embedded samples using nested polymerase chain reaction (PCR) and automated sequencing for HPV detection and genotyping. The results by social and clinicopathologic variables were then compared using the chi-squared test and multivariate analysis. RESULTS: Sociodemographic analysesdemonstrated that most patients were men (87.8%), brown-skinned (75.6%), and resident in the state capital (53.7%). They generally had a poor education status (53.7%), having only an elementary school education (completed/incomplete), and 51.2% were self-employed in occupations such as farming or fishing. Smoking and alcohol consumption habits were observed in approximately half the patients. With respect to clinical characteristics, 39% of patients exhibited T1/T2 staging, 51.2% had no distant metastasis, and 30.5% had lymph node invasion. HPV DNA was detected in half the samples (50%), with the high oncogenic type 16 being the most prevalent. There was no significant relationship observed between the economic, educational, occupational with the HPV LSCC in the presented data, although multivariate analysis demonstrated that HPV DNA was more likely to be present in T3–T4 tumors (p = 0.002). PeerJ Inc. 2022-07-12 /pmc/articles/PMC9285469/ /pubmed/35846883 http://dx.doi.org/10.7717/peerj.13684 Text en ©2022 Brito et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Molecular Biology Brito, Charlles Cossetti, Rachel D. de Souza, Diego Agra Catanha, Marcos de Matos Monteiro, Pablo Vidal, Flavia Castello Branco Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil—a retrospective study |
title | Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil—a retrospective study |
title_full | Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil—a retrospective study |
title_fullStr | Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil—a retrospective study |
title_full_unstemmed | Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil—a retrospective study |
title_short | Prevalence of HPV genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of Brazil—a retrospective study |
title_sort | prevalence of hpv genotypes and assessment of their clinical relevance in laryngeal squamous cell carcinoma in a northeastern state of brazil—a retrospective study |
topic | Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285469/ https://www.ncbi.nlm.nih.gov/pubmed/35846883 http://dx.doi.org/10.7717/peerj.13684 |
work_keys_str_mv | AT britocharlles prevalenceofhpvgenotypesandassessmentoftheirclinicalrelevanceinlaryngealsquamouscellcarcinomainanortheasternstateofbrazilaretrospectivestudy AT cossettiracheld prevalenceofhpvgenotypesandassessmentoftheirclinicalrelevanceinlaryngealsquamouscellcarcinomainanortheasternstateofbrazilaretrospectivestudy AT desouzadiegoagra prevalenceofhpvgenotypesandassessmentoftheirclinicalrelevanceinlaryngealsquamouscellcarcinomainanortheasternstateofbrazilaretrospectivestudy AT catanhamarcos prevalenceofhpvgenotypesandassessmentoftheirclinicalrelevanceinlaryngealsquamouscellcarcinomainanortheasternstateofbrazilaretrospectivestudy AT dematosmonteiropablo prevalenceofhpvgenotypesandassessmentoftheirclinicalrelevanceinlaryngealsquamouscellcarcinomainanortheasternstateofbrazilaretrospectivestudy AT vidalflaviacastellobranco prevalenceofhpvgenotypesandassessmentoftheirclinicalrelevanceinlaryngealsquamouscellcarcinomainanortheasternstateofbrazilaretrospectivestudy |