Preparation of Primary Rat Hepatocyte Spheroids Utilizing the Liquid‐Overlay Technique

Herein, we describe a protocol for the preparation and analysis of primary isolated rat hepatocytes in a 3D cell culture format described as spheroids. The hepatocyte cells spontaneously self‐aggregate into spheroids without the need for synthetic extracellular matrices or hydrogels. Primary rat hep...

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Detalles Bibliográficos
Autores principales: Kyffin, Jonathan A., Cox, Christopher R., Leedale, Joseph, Colley, Helen E., Murdoch, Craig, Mistry, Pratibha, Webb, Steven D., Sharma, Parveen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285795/
https://www.ncbi.nlm.nih.gov/pubmed/31529797
http://dx.doi.org/10.1002/cptx.87

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285795/
https://www.ncbi.nlm.nih.gov/pubmed/31529797
http://dx.doi.org/10.1002/cptx.87

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