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Preparation of Primary Rat Hepatocyte Spheroids Utilizing the Liquid‐Overlay Technique
Herein, we describe a protocol for the preparation and analysis of primary isolated rat hepatocytes in a 3D cell culture format described as spheroids. The hepatocyte cells spontaneously self‐aggregate into spheroids without the need for synthetic extracellular matrices or hydrogels. Primary rat hep...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9285795/ https://www.ncbi.nlm.nih.gov/pubmed/31529797 http://dx.doi.org/10.1002/cptx.87 |