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Cervical lesion proportion measure using a digital gridded imaging technique to assess cervical pathology in women with genital schistosomiasis

Female genital schistosomiasis (FGS) is characterized by a pattern of lesions which manifest at the cervix and the vagina, such as homogeneous and grainy sandy patches, rubbery papules in addition to neovascularization. A tool for quantification of the lesions is needed to improve FGS research and c...

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Autores principales: Arenholt, Louise Thomsen Schmidt, Aaroe, Katrina Kaestel, Norderud, Kanutte, Lumholdt, Mads, Randrianasolo, Bodo Sahondra, Ramarokoto, Charles Emile, Rabozakandraina, Oliva, Broennum, Dorthe, Feldmeier, Hermann, Leutscher, Peter Derek Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286223/
https://www.ncbi.nlm.nih.gov/pubmed/35788749
http://dx.doi.org/10.1371/journal.pntd.0009995
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author Arenholt, Louise Thomsen Schmidt
Aaroe, Katrina Kaestel
Norderud, Kanutte
Lumholdt, Mads
Randrianasolo, Bodo Sahondra
Ramarokoto, Charles Emile
Rabozakandraina, Oliva
Broennum, Dorthe
Feldmeier, Hermann
Leutscher, Peter Derek Christian
author_facet Arenholt, Louise Thomsen Schmidt
Aaroe, Katrina Kaestel
Norderud, Kanutte
Lumholdt, Mads
Randrianasolo, Bodo Sahondra
Ramarokoto, Charles Emile
Rabozakandraina, Oliva
Broennum, Dorthe
Feldmeier, Hermann
Leutscher, Peter Derek Christian
author_sort Arenholt, Louise Thomsen Schmidt
collection PubMed
description Female genital schistosomiasis (FGS) is characterized by a pattern of lesions which manifest at the cervix and the vagina, such as homogeneous and grainy sandy patches, rubbery papules in addition to neovascularization. A tool for quantification of the lesions is needed to improve FGS research and control programs. Hitherto, no tools are available to quantify clinical pathology at the cervix in a standardized and reproducible manner. This study aimed to develop and validate a cervical lesion proportion (CLP) measure for quantification of cervical pathology in FGS. A digital imaging technique was applied in which a grid containing 424 identical squares was positioned on high resolution digital images from the cervix of 70 women with FGS. CLP was measured for each image by observers counting the total number of squares containing at least one type of FGS associated lesion. For assessment of inter- and intra-observer reliability, three different observers measured CLP independently. In addition, a rubbery papule count (RPC) was determined in a similar manner. The intraclass correlation coefficient was 0.94 (excellent) for the CLP inter-rater reliability and 0.90 (good) for intra-rater reliability and the coefficients for the RPC were 0.88 and 0.80 (good), respectively. The CLP facilitated a reliable and reproducible quantification of FGS associated lesions of the cervix. In the future, grading of cervical pathology by CLP may provide insight into the natural course of schistosome egg-induced pathology of the cervix and may have a role in assessing praziquantel treatment efficacy against FGS. Trial Registration: ClinicalTrials.gov, trial number NCT04115072; trial URL https://clinicaltrials.gov/ct2/show/NCT04115072?term=Female+genital+schistosomiasis+AND+Madagascar&draw=2&rank=1.
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spelling pubmed-92862232022-07-16 Cervical lesion proportion measure using a digital gridded imaging technique to assess cervical pathology in women with genital schistosomiasis Arenholt, Louise Thomsen Schmidt Aaroe, Katrina Kaestel Norderud, Kanutte Lumholdt, Mads Randrianasolo, Bodo Sahondra Ramarokoto, Charles Emile Rabozakandraina, Oliva Broennum, Dorthe Feldmeier, Hermann Leutscher, Peter Derek Christian PLoS Negl Trop Dis Research Article Female genital schistosomiasis (FGS) is characterized by a pattern of lesions which manifest at the cervix and the vagina, such as homogeneous and grainy sandy patches, rubbery papules in addition to neovascularization. A tool for quantification of the lesions is needed to improve FGS research and control programs. Hitherto, no tools are available to quantify clinical pathology at the cervix in a standardized and reproducible manner. This study aimed to develop and validate a cervical lesion proportion (CLP) measure for quantification of cervical pathology in FGS. A digital imaging technique was applied in which a grid containing 424 identical squares was positioned on high resolution digital images from the cervix of 70 women with FGS. CLP was measured for each image by observers counting the total number of squares containing at least one type of FGS associated lesion. For assessment of inter- and intra-observer reliability, three different observers measured CLP independently. In addition, a rubbery papule count (RPC) was determined in a similar manner. The intraclass correlation coefficient was 0.94 (excellent) for the CLP inter-rater reliability and 0.90 (good) for intra-rater reliability and the coefficients for the RPC were 0.88 and 0.80 (good), respectively. The CLP facilitated a reliable and reproducible quantification of FGS associated lesions of the cervix. In the future, grading of cervical pathology by CLP may provide insight into the natural course of schistosome egg-induced pathology of the cervix and may have a role in assessing praziquantel treatment efficacy against FGS. Trial Registration: ClinicalTrials.gov, trial number NCT04115072; trial URL https://clinicaltrials.gov/ct2/show/NCT04115072?term=Female+genital+schistosomiasis+AND+Madagascar&draw=2&rank=1. Public Library of Science 2022-07-05 /pmc/articles/PMC9286223/ /pubmed/35788749 http://dx.doi.org/10.1371/journal.pntd.0009995 Text en © 2022 Arenholt et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Arenholt, Louise Thomsen Schmidt
Aaroe, Katrina Kaestel
Norderud, Kanutte
Lumholdt, Mads
Randrianasolo, Bodo Sahondra
Ramarokoto, Charles Emile
Rabozakandraina, Oliva
Broennum, Dorthe
Feldmeier, Hermann
Leutscher, Peter Derek Christian
Cervical lesion proportion measure using a digital gridded imaging technique to assess cervical pathology in women with genital schistosomiasis
title Cervical lesion proportion measure using a digital gridded imaging technique to assess cervical pathology in women with genital schistosomiasis
title_full Cervical lesion proportion measure using a digital gridded imaging technique to assess cervical pathology in women with genital schistosomiasis
title_fullStr Cervical lesion proportion measure using a digital gridded imaging technique to assess cervical pathology in women with genital schistosomiasis
title_full_unstemmed Cervical lesion proportion measure using a digital gridded imaging technique to assess cervical pathology in women with genital schistosomiasis
title_short Cervical lesion proportion measure using a digital gridded imaging technique to assess cervical pathology in women with genital schistosomiasis
title_sort cervical lesion proportion measure using a digital gridded imaging technique to assess cervical pathology in women with genital schistosomiasis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286223/
https://www.ncbi.nlm.nih.gov/pubmed/35788749
http://dx.doi.org/10.1371/journal.pntd.0009995
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