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A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates

Tuberculosis is a respiratory disease that is treatable with antibiotics. An increasing prevalence of resistance means that to ensure a good treatment outcome it is desirable to test the susceptibility of each infection to different antibiotics. Conventionally, this is done by culturing a clinical s...

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Autores principales: Fowler, Philip W, Wright, Carla, Spiers, Helen, Zhu, Tingting, Baeten, Elisabeth ML, Hoosdally, Sarah W, Gibertoni Cruz, Ana L, Roohi, Aysha, Kouchaki, Samaneh, Walker, Timothy M, Peto, Timothy EA, Miller, Grant, Lintott, Chris, Clifton, David, Crook, Derrick W, Walker, A Sarah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286738/
https://www.ncbi.nlm.nih.gov/pubmed/35588296
http://dx.doi.org/10.7554/eLife.75046
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author Fowler, Philip W
Wright, Carla
Spiers, Helen
Zhu, Tingting
Baeten, Elisabeth ML
Hoosdally, Sarah W
Gibertoni Cruz, Ana L
Roohi, Aysha
Kouchaki, Samaneh
Walker, Timothy M
Peto, Timothy EA
Miller, Grant
Lintott, Chris
Clifton, David
Crook, Derrick W
Walker, A Sarah
author_facet Fowler, Philip W
Wright, Carla
Spiers, Helen
Zhu, Tingting
Baeten, Elisabeth ML
Hoosdally, Sarah W
Gibertoni Cruz, Ana L
Roohi, Aysha
Kouchaki, Samaneh
Walker, Timothy M
Peto, Timothy EA
Miller, Grant
Lintott, Chris
Clifton, David
Crook, Derrick W
Walker, A Sarah
author_sort Fowler, Philip W
collection PubMed
description Tuberculosis is a respiratory disease that is treatable with antibiotics. An increasing prevalence of resistance means that to ensure a good treatment outcome it is desirable to test the susceptibility of each infection to different antibiotics. Conventionally, this is done by culturing a clinical sample and then exposing aliquots to a panel of antibiotics, each being present at a pre-determined concentration, thereby determining if the sample isresistant or susceptible to each sample. The minimum inhibitory concentration (MIC) of a drug is the lowestconcentration that inhibits growth and is a more useful quantity but requires each sample to be tested at a range ofconcentrations for each drug. Using 96-well broth micro dilution plates with each well containing a lyophilised pre-determined amount of an antibiotic is a convenient and cost-effective way to measure the MICs of several drugs at once for a clinical sample. Although accurate, this is still an expensive and slow process that requires highly-skilled and experienced laboratory scientists. Here we show that, through the BashTheBug project hosted on the Zooniverse citizen science platform, a crowd of volunteers can reproducibly and accurately determine the MICs for 13 drugs and that simply taking the median or mode of 11–17 independent classifications is sufficient. There is therefore a potential role for crowds to support (but not supplant) the role of experts in antibiotic susceptibility testing.
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spelling pubmed-92867382022-07-16 A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates Fowler, Philip W Wright, Carla Spiers, Helen Zhu, Tingting Baeten, Elisabeth ML Hoosdally, Sarah W Gibertoni Cruz, Ana L Roohi, Aysha Kouchaki, Samaneh Walker, Timothy M Peto, Timothy EA Miller, Grant Lintott, Chris Clifton, David Crook, Derrick W Walker, A Sarah eLife Microbiology and Infectious Disease Tuberculosis is a respiratory disease that is treatable with antibiotics. An increasing prevalence of resistance means that to ensure a good treatment outcome it is desirable to test the susceptibility of each infection to different antibiotics. Conventionally, this is done by culturing a clinical sample and then exposing aliquots to a panel of antibiotics, each being present at a pre-determined concentration, thereby determining if the sample isresistant or susceptible to each sample. The minimum inhibitory concentration (MIC) of a drug is the lowestconcentration that inhibits growth and is a more useful quantity but requires each sample to be tested at a range ofconcentrations for each drug. Using 96-well broth micro dilution plates with each well containing a lyophilised pre-determined amount of an antibiotic is a convenient and cost-effective way to measure the MICs of several drugs at once for a clinical sample. Although accurate, this is still an expensive and slow process that requires highly-skilled and experienced laboratory scientists. Here we show that, through the BashTheBug project hosted on the Zooniverse citizen science platform, a crowd of volunteers can reproducibly and accurately determine the MICs for 13 drugs and that simply taking the median or mode of 11–17 independent classifications is sufficient. There is therefore a potential role for crowds to support (but not supplant) the role of experts in antibiotic susceptibility testing. eLife Sciences Publications, Ltd 2022-05-19 /pmc/articles/PMC9286738/ /pubmed/35588296 http://dx.doi.org/10.7554/eLife.75046 Text en © 2022, Fowler et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Fowler, Philip W
Wright, Carla
Spiers, Helen
Zhu, Tingting
Baeten, Elisabeth ML
Hoosdally, Sarah W
Gibertoni Cruz, Ana L
Roohi, Aysha
Kouchaki, Samaneh
Walker, Timothy M
Peto, Timothy EA
Miller, Grant
Lintott, Chris
Clifton, David
Crook, Derrick W
Walker, A Sarah
A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates
title A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates
title_full A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates
title_fullStr A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates
title_full_unstemmed A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates
title_short A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates
title_sort crowd of bashthebug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286738/
https://www.ncbi.nlm.nih.gov/pubmed/35588296
http://dx.doi.org/10.7554/eLife.75046
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