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A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates
Tuberculosis is a respiratory disease that is treatable with antibiotics. An increasing prevalence of resistance means that to ensure a good treatment outcome it is desirable to test the susceptibility of each infection to different antibiotics. Conventionally, this is done by culturing a clinical s...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286738/ https://www.ncbi.nlm.nih.gov/pubmed/35588296 http://dx.doi.org/10.7554/eLife.75046 |
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author | Fowler, Philip W Wright, Carla Spiers, Helen Zhu, Tingting Baeten, Elisabeth ML Hoosdally, Sarah W Gibertoni Cruz, Ana L Roohi, Aysha Kouchaki, Samaneh Walker, Timothy M Peto, Timothy EA Miller, Grant Lintott, Chris Clifton, David Crook, Derrick W Walker, A Sarah |
author_facet | Fowler, Philip W Wright, Carla Spiers, Helen Zhu, Tingting Baeten, Elisabeth ML Hoosdally, Sarah W Gibertoni Cruz, Ana L Roohi, Aysha Kouchaki, Samaneh Walker, Timothy M Peto, Timothy EA Miller, Grant Lintott, Chris Clifton, David Crook, Derrick W Walker, A Sarah |
author_sort | Fowler, Philip W |
collection | PubMed |
description | Tuberculosis is a respiratory disease that is treatable with antibiotics. An increasing prevalence of resistance means that to ensure a good treatment outcome it is desirable to test the susceptibility of each infection to different antibiotics. Conventionally, this is done by culturing a clinical sample and then exposing aliquots to a panel of antibiotics, each being present at a pre-determined concentration, thereby determining if the sample isresistant or susceptible to each sample. The minimum inhibitory concentration (MIC) of a drug is the lowestconcentration that inhibits growth and is a more useful quantity but requires each sample to be tested at a range ofconcentrations for each drug. Using 96-well broth micro dilution plates with each well containing a lyophilised pre-determined amount of an antibiotic is a convenient and cost-effective way to measure the MICs of several drugs at once for a clinical sample. Although accurate, this is still an expensive and slow process that requires highly-skilled and experienced laboratory scientists. Here we show that, through the BashTheBug project hosted on the Zooniverse citizen science platform, a crowd of volunteers can reproducibly and accurately determine the MICs for 13 drugs and that simply taking the median or mode of 11–17 independent classifications is sufficient. There is therefore a potential role for crowds to support (but not supplant) the role of experts in antibiotic susceptibility testing. |
format | Online Article Text |
id | pubmed-9286738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-92867382022-07-16 A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates Fowler, Philip W Wright, Carla Spiers, Helen Zhu, Tingting Baeten, Elisabeth ML Hoosdally, Sarah W Gibertoni Cruz, Ana L Roohi, Aysha Kouchaki, Samaneh Walker, Timothy M Peto, Timothy EA Miller, Grant Lintott, Chris Clifton, David Crook, Derrick W Walker, A Sarah eLife Microbiology and Infectious Disease Tuberculosis is a respiratory disease that is treatable with antibiotics. An increasing prevalence of resistance means that to ensure a good treatment outcome it is desirable to test the susceptibility of each infection to different antibiotics. Conventionally, this is done by culturing a clinical sample and then exposing aliquots to a panel of antibiotics, each being present at a pre-determined concentration, thereby determining if the sample isresistant or susceptible to each sample. The minimum inhibitory concentration (MIC) of a drug is the lowestconcentration that inhibits growth and is a more useful quantity but requires each sample to be tested at a range ofconcentrations for each drug. Using 96-well broth micro dilution plates with each well containing a lyophilised pre-determined amount of an antibiotic is a convenient and cost-effective way to measure the MICs of several drugs at once for a clinical sample. Although accurate, this is still an expensive and slow process that requires highly-skilled and experienced laboratory scientists. Here we show that, through the BashTheBug project hosted on the Zooniverse citizen science platform, a crowd of volunteers can reproducibly and accurately determine the MICs for 13 drugs and that simply taking the median or mode of 11–17 independent classifications is sufficient. There is therefore a potential role for crowds to support (but not supplant) the role of experts in antibiotic susceptibility testing. eLife Sciences Publications, Ltd 2022-05-19 /pmc/articles/PMC9286738/ /pubmed/35588296 http://dx.doi.org/10.7554/eLife.75046 Text en © 2022, Fowler et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease Fowler, Philip W Wright, Carla Spiers, Helen Zhu, Tingting Baeten, Elisabeth ML Hoosdally, Sarah W Gibertoni Cruz, Ana L Roohi, Aysha Kouchaki, Samaneh Walker, Timothy M Peto, Timothy EA Miller, Grant Lintott, Chris Clifton, David Crook, Derrick W Walker, A Sarah A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates |
title | A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates |
title_full | A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates |
title_fullStr | A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates |
title_full_unstemmed | A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates |
title_short | A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates |
title_sort | crowd of bashthebug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286738/ https://www.ncbi.nlm.nih.gov/pubmed/35588296 http://dx.doi.org/10.7554/eLife.75046 |
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