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Biological evaluation of acellular bovine bone matrix treated with NaOH

We mainly proceed from the view of biological effect to study the acellular bovine bone matrix (ABBM) by the low concentration of hydrogen oxidation. After cleaning the bovine bone routinely, it was cleaned with different concentrations of NaOH and stained with hematoxylin-eosin (HE) to observe the...

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Autores principales: Li, Pengfei, Feng, Mengchun, Hu, Xiantong, Zhang, Chunli, Zhu, Jialiang, Xu, Gang, Li, Li, Zhao, Yantao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287214/
https://www.ncbi.nlm.nih.gov/pubmed/35838844
http://dx.doi.org/10.1007/s10856-022-06678-z
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author Li, Pengfei
Feng, Mengchun
Hu, Xiantong
Zhang, Chunli
Zhu, Jialiang
Xu, Gang
Li, Li
Zhao, Yantao
author_facet Li, Pengfei
Feng, Mengchun
Hu, Xiantong
Zhang, Chunli
Zhu, Jialiang
Xu, Gang
Li, Li
Zhao, Yantao
author_sort Li, Pengfei
collection PubMed
description We mainly proceed from the view of biological effect to study the acellular bovine bone matrix (ABBM) by the low concentration of hydrogen oxidation. After cleaning the bovine bone routinely, it was cleaned with different concentrations of NaOH and stained with hematoxylin-eosin (HE) to observe the effect of decellulization. The effect of bovine bone matrix treated with NaOH were observed by optical microscopy and scanning electron microscopy (SEM), and compared by DNA residue detection. Cell toxicity was also evaluated in MC3T3-E1 cells by CCK-8. For the in vitro osteogenesis detection, alkaline phosphatase (ALP) staining and alizarin red (AR) staining were performed in MC3T3-E1 cells. And the in vivo experiment, Micro CT, HE and Masson staining were used to observe whether the osteogenic effect of the materials treated with 1% NaOH solution was affected at 6 and 12 weeks. After the bovine bone was decellularized with different concentrations of NaOH solution, HE staining showed that ultrasonic cleaning with 1% NaOH solution for 30 min had the best effect of decellularization. The SEM showed that ABBM treated with 1% NaOH solution had few residual cells on the surface of the three-dimensional porous compared to ABBM treated with conventional chemical reagents. DNA residues and cytotoxicity of ABBM treated with 1% NaOH were both reduced. The results of ALP staining and AR staining showed that ABBM treated with 1% NaOH solution had no effect on the osteogenesis effect. The results of micro-CT, HE staining and Masson staining in animal experiments also showed that ABBM treated with 1% NaOH solution had no effect on the osteogenesis ability. The decellularization treatment of ABBM with the low concentration of NaOH can be more cost-effective, effectively remove the residual cellular components, without affecting the osteogenic ability. Our work may provide a novelty thought and a modified method to applicate the acellular bovine bone matrix clinically better. [Figure: see text]
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spelling pubmed-92872142022-07-17 Biological evaluation of acellular bovine bone matrix treated with NaOH Li, Pengfei Feng, Mengchun Hu, Xiantong Zhang, Chunli Zhu, Jialiang Xu, Gang Li, Li Zhao, Yantao J Mater Sci Mater Med Biocompatibility Studies We mainly proceed from the view of biological effect to study the acellular bovine bone matrix (ABBM) by the low concentration of hydrogen oxidation. After cleaning the bovine bone routinely, it was cleaned with different concentrations of NaOH and stained with hematoxylin-eosin (HE) to observe the effect of decellulization. The effect of bovine bone matrix treated with NaOH were observed by optical microscopy and scanning electron microscopy (SEM), and compared by DNA residue detection. Cell toxicity was also evaluated in MC3T3-E1 cells by CCK-8. For the in vitro osteogenesis detection, alkaline phosphatase (ALP) staining and alizarin red (AR) staining were performed in MC3T3-E1 cells. And the in vivo experiment, Micro CT, HE and Masson staining were used to observe whether the osteogenic effect of the materials treated with 1% NaOH solution was affected at 6 and 12 weeks. After the bovine bone was decellularized with different concentrations of NaOH solution, HE staining showed that ultrasonic cleaning with 1% NaOH solution for 30 min had the best effect of decellularization. The SEM showed that ABBM treated with 1% NaOH solution had few residual cells on the surface of the three-dimensional porous compared to ABBM treated with conventional chemical reagents. DNA residues and cytotoxicity of ABBM treated with 1% NaOH were both reduced. The results of ALP staining and AR staining showed that ABBM treated with 1% NaOH solution had no effect on the osteogenesis effect. The results of micro-CT, HE staining and Masson staining in animal experiments also showed that ABBM treated with 1% NaOH solution had no effect on the osteogenesis ability. The decellularization treatment of ABBM with the low concentration of NaOH can be more cost-effective, effectively remove the residual cellular components, without affecting the osteogenic ability. Our work may provide a novelty thought and a modified method to applicate the acellular bovine bone matrix clinically better. [Figure: see text] Springer US 2022-07-15 2022 /pmc/articles/PMC9287214/ /pubmed/35838844 http://dx.doi.org/10.1007/s10856-022-06678-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biocompatibility Studies
Li, Pengfei
Feng, Mengchun
Hu, Xiantong
Zhang, Chunli
Zhu, Jialiang
Xu, Gang
Li, Li
Zhao, Yantao
Biological evaluation of acellular bovine bone matrix treated with NaOH
title Biological evaluation of acellular bovine bone matrix treated with NaOH
title_full Biological evaluation of acellular bovine bone matrix treated with NaOH
title_fullStr Biological evaluation of acellular bovine bone matrix treated with NaOH
title_full_unstemmed Biological evaluation of acellular bovine bone matrix treated with NaOH
title_short Biological evaluation of acellular bovine bone matrix treated with NaOH
title_sort biological evaluation of acellular bovine bone matrix treated with naoh
topic Biocompatibility Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287214/
https://www.ncbi.nlm.nih.gov/pubmed/35838844
http://dx.doi.org/10.1007/s10856-022-06678-z
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