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Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana
A novel Gram-stain-positive, aerobic bacterial strain, designated AK-R2A1-2( T), was isolated from the surface-sterilized needle leaves of an Abies koreana tree. Strain AK-R2A1-2( T) had 97.3% and 96.7% 16S rRNA gene sequence similarities with Subtercola boreus K300(T) and Subtercola lobariae 9583b(...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287328/ https://www.ncbi.nlm.nih.gov/pubmed/35840645 http://dx.doi.org/10.1038/s41598-022-16116-3 |
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author | Jiang, Lingmin Peng, Yuxin Seo, Jiyoon Jeon, Doeun Jo, Mi Gyeong Lee, Ju Huck Jeong, Jae Cheol Kim, Cha Young Park, Hyeong Cheol Lee, Jiyoung |
author_facet | Jiang, Lingmin Peng, Yuxin Seo, Jiyoon Jeon, Doeun Jo, Mi Gyeong Lee, Ju Huck Jeong, Jae Cheol Kim, Cha Young Park, Hyeong Cheol Lee, Jiyoung |
author_sort | Jiang, Lingmin |
collection | PubMed |
description | A novel Gram-stain-positive, aerobic bacterial strain, designated AK-R2A1-2( T), was isolated from the surface-sterilized needle leaves of an Abies koreana tree. Strain AK-R2A1-2( T) had 97.3% and 96.7% 16S rRNA gene sequence similarities with Subtercola boreus K300(T) and Subtercola lobariae 9583b(T), respectively, but formed a distinct phyletic lineage from these two strains. Growth of strain AK-R2A1-2( T) was observed at 4–25 °C at pH 5.0–8.0. Strain AK-R2A1-2( T) contained menaquinone 9 (MK-9) and menaquinone 10 (MK-10) as the predominant respiratory quinones. The major cellular fatty acids were anteiso-C(15:0) and summed feature 8 (C(18:1)ω7c or/and C(18:1)ω6c), and the polar lipids included diphosphatidylglycerol (DPG) and three unknown aminolipids, AKL2, AKL3, and AKL4. The complete genome of strain AK-R2A1-2( T) was sequenced to understand the genetic basis of its survival at low temperatures. Multiple copies of cold-associated genes involved in cold-active chaperon, stress response, and DNA repair supported survival of the strain at low temperatures. Strain AK-R2A1-2( T) was also able to significantly improve rice seedling growth under low temperatures. Thus, this strain represents a novel species of the genus Subtercola, and the proposed name is Subtercola endophyticus sp. nov. The type strain is AK-R2A1-2( T) (= KCTC 49721( T) = GDMCC 1.2921( T)). |
format | Online Article Text |
id | pubmed-9287328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-92873282022-07-17 Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana Jiang, Lingmin Peng, Yuxin Seo, Jiyoon Jeon, Doeun Jo, Mi Gyeong Lee, Ju Huck Jeong, Jae Cheol Kim, Cha Young Park, Hyeong Cheol Lee, Jiyoung Sci Rep Article A novel Gram-stain-positive, aerobic bacterial strain, designated AK-R2A1-2( T), was isolated from the surface-sterilized needle leaves of an Abies koreana tree. Strain AK-R2A1-2( T) had 97.3% and 96.7% 16S rRNA gene sequence similarities with Subtercola boreus K300(T) and Subtercola lobariae 9583b(T), respectively, but formed a distinct phyletic lineage from these two strains. Growth of strain AK-R2A1-2( T) was observed at 4–25 °C at pH 5.0–8.0. Strain AK-R2A1-2( T) contained menaquinone 9 (MK-9) and menaquinone 10 (MK-10) as the predominant respiratory quinones. The major cellular fatty acids were anteiso-C(15:0) and summed feature 8 (C(18:1)ω7c or/and C(18:1)ω6c), and the polar lipids included diphosphatidylglycerol (DPG) and three unknown aminolipids, AKL2, AKL3, and AKL4. The complete genome of strain AK-R2A1-2( T) was sequenced to understand the genetic basis of its survival at low temperatures. Multiple copies of cold-associated genes involved in cold-active chaperon, stress response, and DNA repair supported survival of the strain at low temperatures. Strain AK-R2A1-2( T) was also able to significantly improve rice seedling growth under low temperatures. Thus, this strain represents a novel species of the genus Subtercola, and the proposed name is Subtercola endophyticus sp. nov. The type strain is AK-R2A1-2( T) (= KCTC 49721( T) = GDMCC 1.2921( T)). Nature Publishing Group UK 2022-07-15 /pmc/articles/PMC9287328/ /pubmed/35840645 http://dx.doi.org/10.1038/s41598-022-16116-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Jiang, Lingmin Peng, Yuxin Seo, Jiyoon Jeon, Doeun Jo, Mi Gyeong Lee, Ju Huck Jeong, Jae Cheol Kim, Cha Young Park, Hyeong Cheol Lee, Jiyoung Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana |
title | Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana |
title_full | Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana |
title_fullStr | Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana |
title_full_unstemmed | Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana |
title_short | Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana |
title_sort | subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from abies koreana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287328/ https://www.ncbi.nlm.nih.gov/pubmed/35840645 http://dx.doi.org/10.1038/s41598-022-16116-3 |
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