A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution

Innate lymphoid cells (ILCs) and adaptive T cells remain a challenge to study because of a significant overlap in their transcriptomic profiles. Here, we describe the adoptive transfer of ILC progenitors into mice genetically deficient in innate and adaptive immune cells to allow detailed study of t...

Descripción completa

Detalles Bibliográficos
Autores principales: Jacquelot, Nicolas, Huang, Qiutong, Belz, Gabrielle T., Seillet, Cyril
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287798/
https://www.ncbi.nlm.nih.gov/pubmed/35830307
http://dx.doi.org/10.1016/j.xpro.2022.101534
_version_ 1784748327485571072
author Jacquelot, Nicolas
Huang, Qiutong
Belz, Gabrielle T.
Seillet, Cyril
author_facet Jacquelot, Nicolas
Huang, Qiutong
Belz, Gabrielle T.
Seillet, Cyril
author_sort Jacquelot, Nicolas
collection PubMed
description Innate lymphoid cells (ILCs) and adaptive T cells remain a challenge to study because of a significant overlap in their transcriptomic profiles. Here, we describe the adoptive transfer of ILC progenitors into mice genetically deficient in innate and adaptive immune cells to allow detailed study of the development and function of ILCs and gene regulation in an in vivo setting. For complete details on the use and execution of this protocol, please refer to Jacquelot et al. (2021) and Seillet et al. (2016).
format Online
Article
Text
id pubmed-9287798
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92877982022-07-17 A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution Jacquelot, Nicolas Huang, Qiutong Belz, Gabrielle T. Seillet, Cyril STAR Protoc Protocol Innate lymphoid cells (ILCs) and adaptive T cells remain a challenge to study because of a significant overlap in their transcriptomic profiles. Here, we describe the adoptive transfer of ILC progenitors into mice genetically deficient in innate and adaptive immune cells to allow detailed study of the development and function of ILCs and gene regulation in an in vivo setting. For complete details on the use and execution of this protocol, please refer to Jacquelot et al. (2021) and Seillet et al. (2016). Elsevier 2022-07-12 /pmc/articles/PMC9287798/ /pubmed/35830307 http://dx.doi.org/10.1016/j.xpro.2022.101534 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Jacquelot, Nicolas
Huang, Qiutong
Belz, Gabrielle T.
Seillet, Cyril
A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
title A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
title_full A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
title_fullStr A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
title_full_unstemmed A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
title_short A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
title_sort protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287798/
https://www.ncbi.nlm.nih.gov/pubmed/35830307
http://dx.doi.org/10.1016/j.xpro.2022.101534
work_keys_str_mv AT jacquelotnicolas aprotocoltoisolatebonemarrowinnatelymphoidcellsforalymphoidmousereconstitution
AT huangqiutong aprotocoltoisolatebonemarrowinnatelymphoidcellsforalymphoidmousereconstitution
AT belzgabriellet aprotocoltoisolatebonemarrowinnatelymphoidcellsforalymphoidmousereconstitution
AT seilletcyril aprotocoltoisolatebonemarrowinnatelymphoidcellsforalymphoidmousereconstitution
AT jacquelotnicolas protocoltoisolatebonemarrowinnatelymphoidcellsforalymphoidmousereconstitution
AT huangqiutong protocoltoisolatebonemarrowinnatelymphoidcellsforalymphoidmousereconstitution
AT belzgabriellet protocoltoisolatebonemarrowinnatelymphoidcellsforalymphoidmousereconstitution
AT seilletcyril protocoltoisolatebonemarrowinnatelymphoidcellsforalymphoidmousereconstitution

Ejemplares similares